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Laser Photo-Fabrication of Metallic Nanoparticles in Living Cells

Research Project

Project/Area Number 20760035
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied optics/Quantum optical engineering
Research InstitutionOsaka University

Principal Investigator

スミス ニコラス アイザック  Osaka University, 免疫学フロンティア研究センター, 特任講師 (10362592)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsナノ粒子 / フォトファブリケーション / 培養細胞 / 毒性 / 生存能力 / 表面増強ラマン / ナノ粒子製造 / 光還元 / 細胞生物学 / 表面増強ラマン散乱
Research Abstract

This research has found that metal can be fabricated in the salt conditions of the cell, but localization remains difficult, using multiphoton laser irradiation. However, the cells were found to exclude indicator propidium iodide, which is very promising for the future continuation of this work. Using dark-field and luminescence imaging, highly scattering regions were observed around the location of the laser focus, consistent with gold nanoparticles.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (7 results)

All 2009 2008 Other

All Journal Article (5 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Time-resolved observation of surface-enhanced Raman scattering from gold nanoparticles during transport through a living cell2009

    • Author(s)
      K. Fujita, S. Ishitobi, K. Hamada, N.I. Smith, A. Taguchi, Y. Inouye, S. Kawata
    • Journal Title

      J. Biomed. Opt. PEER REVIEWED VOLUME14,ISSUED

      Pages: 24038-24038

    • Related Report
      2009 Final Research Report
  • [Journal Article] Photogeneration of membrane potential hyperpolarization and depolarization in non-excitable cells2009

    • Author(s)
      J. Ando, NI Smith, K. Fujita, S. Kawata
    • Journal Title

      Eur. Biophys. J. PEER VOLUME38(2)(REVIEWER)

      Pages: 255-262

    • Related Report
      2009 Final Research Report
  • [Journal Article] Optical trapping and surgery of living yeast cells using a single laser2008

    • Author(s)
      J. Ando, G. Bautista, N. Smith, K. Fujita, V.R. Daria
    • Journal Title

      Rev. Sci. Instrum. VOLUME79(PEER REVIEWED)

      Pages: 103705-103705

    • Related Report
      2009 Final Research Report
  • [Journal Article]2008

    • Author(s)
      Keisaku Hamada, Katsumasa Fujita, Nicholas Isaac Smith, Minoru Kobayashi, Yasushi Inouye, Satoshi Kawata
    • Journal Title

      J. Biomed. Opt. VOLUME13(PEER REVIEWED)

      Pages: 44027-44027

    • Related Report
      2009 Final Research Report
  • [Journal Article] Time-resolved observation of surface-enhanced Raman scattering from gold nanoparticles during transport through a living cell

    • Author(s)
      SMITH Nicholas, Isaac
    • Journal Title

      Journal of Biomedical Optics (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 生細胞内での金イオン還元による金ナノ粒子の形成2008

    • Author(s)
      SIMITH Nicholas, Isaac
    • Organizer
      日本生物物理学会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] 生細胞内での金イオン還元による金ナノ粒子の形成2008

    • Author(s)
      森田寛之、新岡宏彦、安藤潤、藤田克昌、河田聡
    • Organizer
      日本生物物理学会年会
    • Place of Presentation
      福岡コンベンションセンター
    • Related Report
      2009 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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