Highly selective pump-dump imaging with a shaped femtosecond pulse train

• Project/Area Number: 20760037
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied optics/Quantum optical engineering
• Research Institution: Tohoku University
• Principal Investigator: NAKAMURA Ryosuke Tohoku University, 大学院・理学研究科, 助教 (70379147)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 超短パルス / 波形整形 / 蛍光イメージング / 誘導放出 / 成分分離 / 超短光パルス / パルス整形

Research Abstract

We have demonstrated that the selective excitation and de-excitation (or re-excitation) method of a fluorescence molecule using femtosecond pulse train with multi colors, which is applicable to highly selective fluorescence imaging of a biological cell and its extra cellular matrix. We constructed the pulse shaping system using two dimensional spatial light modulator and studied suitable pulse trains for the electronic and vibrational character of a target fluorescence molecule.

Research Products

• [Journal Article] High-resolution spatial and temporal analysis of phytoalexin production in oats (2009)
  • Author(s): Y. Izumi, S. Kajiyama, R. Nakamura, A. Ishihara, A. Okazawa, E. Fukusaki, Y. Kanematsu, A. Kobayashi
  • Journal Title: Planta 229 Pages: 931-943
  • Peer Reviewed
• [Journal Article] Vibrational relaxation and internal conversion in the overlapped optically allowed 1Bu+ and optically-forbidden 1Bu- or 3Ag- vibronic levels: Effects of diabatic mixing as determined by Kerr-gate fluorescence spectroscopy (2009)
  • Author(s): Y. Kakitani, T. Miki, Y. Koyama, H. Nagae, R. Nakamura, Y. Kanematsu
  • Journal Title: Chem. Phys. Lett. 477 Pages: 194-201
  • Peer Reviewed
• [Journal Article] Vibrational relaxation and internal conversion in the overlapped optically allowed 1Bu^+ and optically-forbidden 1Bu^- or 3Ag^- vibronic levels : Effects of diabatic mixing as determined by Kerr-gate fluorescence spectroscopy (2009)
  • Author(s): Y.Kakitani, T.Miki, Y.Koyama, H.Nagae, R.Nakamura, Y.Kanematsu
  • Journal Title: Chem.Phys.Lett. 477 Pages: 194-201
  • Peer Reviewed
• [Journal Article] High-resolution spatial and temporal analysis of phytoalexin productionin oats (2009)
  • Author(s): Y. Izumi
  • Journal Title: Planta 229 Pages: 931-943
  • Peer Reviewed
• [Journal Article] Line-scanning microscopy for time-gated and spectrally-resolved fluorescence imaging (2008)
  • Author(s): R. Nakamura, Y. Izumi, S. Kajiyama, A. Kobayashi, Y. Kanematsu
  • Journal Title: J. Biol. Phys. 34 Pages: 51-62
  • Peer Reviewed
• [Journal Article] Line-scanning microscopy for time-gated and spectrally-resolved fluorescence imaging (2008)
  • Author(s): R. Nakamura
  • Journal Title: J. Biol. Phys. 34 Pages: 51-62
  • Peer Reviewed
• [Presentation] マルチパルス光励起による励起状態の分子振動制御 (2010)
  • Author(s): 阿部健太, 中村亮介, 橋本秀樹, 吉澤雅幸
  • Organizer: 日本物理学会第65回年次大会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-23
• [Presentation] Pump-Dump-蛍光スペクトルにおけるRed-edge dumping[IV] (2010)
  • Author(s): 市田秀樹, 中村亮介, 濱田格雄, 兼松泰男
  • Organizer: 日本物理学会第65回年次大会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-23
• [Presentation] Pump-Dump-蛍光スペクトルにおける Red-edge dumping[IV] (2010)
  • Author(s): 市田秀樹, 中村亮介, 濱田格雄, 兼松泰男
  • Organizer: 日本物理学会第65回年次大会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-23
• [Presentation] アクセプタ分子に対する光変調法を用いた光合成エネルギー移動解析 (2009)
  • Author(s): 中村亮介, 中川勝統, 橋本秀樹, 南後守, 吉澤雅幸
  • Organizer: 第20回光物性研究会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-11
• [Presentation] 波形制御された光パルスによる分子振動の制御と観測 (2009)
  • Author(s): 阿部健太, 中村亮介, 吉澤雅幸
  • Organizer: 日本物理学会2009年秋季大会
  • Place of Presentation: 熊本
  • Year and Date: 2009-09-27
• [Presentation] マルチパルス光励起による光合成アンテナ複合体のエネルギー移動解析 (2009)
  • Author(s): 中村亮介, 中川勝統, 橋本秀樹, 南後守, 吉澤雅幸
  • Organizer: 日本物理学会2009年秋季大会
  • Place of Presentation: 熊本
  • Year and Date: 2009-09-27
• [Presentation] Pump-Dump-蛍光スペクトルにおけるRed-edge dumping[III] (2009)
  • Author(s): 市田秀樹, 中村亮介, 濱田格雄, 兼松泰男
  • Organizer: 日本物理学会2009年秋季大会
  • Place of Presentation: 熊本
  • Year and Date: 2009-09-27
• [Presentation] Pump-Dump-蛍光スペクトルにおける Red-edge dumping[III] (2009)
  • Author(s): 市田秀樹, 中村亮介, 濱田格雄, 兼松泰男
  • Organizer: 日本物理学会2009年秋季大会
  • Place of Presentation: 熊本
  • Year and Date: 2009-09-27
• [Presentation] Pump-Dump-蛍光スペクトルにおけるRed-edge dumping [I] (2009)
  • Author(s): 市田秀樹, 中村亮介, 濱田格雄, 兼松泰男
  • Organizer: 日本物理学会第64回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-30
• [Presentation] Pump-Dump-蛍光スペクトルにおけるRed-edge dumping[I] (2009)
  • Author(s): 市田秀樹
  • Organizer: 日本物理学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-30
• [Presentation] Pump-Dump-蛍光スペクトルにおけるRed-edge dumping[II] (2009)
  • Author(s): 兼松泰男, 市田秀樹, 濱田格雄, 中村亮介
  • Organizer: 日本物理学会第64回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-28
• [Presentation] Pump-Dump-蛍光スペクトルにおけるRed-edge dumping[II] (2009)
  • Author(s): 兼松泰男
  • Organizer: 日本物理学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-28
• [Presentation] 分子認識を目指したPump-Dump-蛍光スペクトルにおけるRed-edge Dumping (2009)
  • Author(s): 市田秀樹, 中村亮介, 濱田格雄, 兼松泰男
  • Organizer: レーザー学会学術講演会第29回年次大会
  • Place of Presentation: 徳島
  • Year and Date: 2009-01-10
• [Presentation] 分子認識を目指したPump-Dump-蛍光スペクトルにおけるRed-edge Dumping (2009)
  • Author(s): 市田秀樹
  • Organizer: レーザー学会
  • Place of Presentation: 徳島
  • Year and Date: 2009-01-10
• [Presentation] 整形光パルス列による分子認識 (2008)
  • Author(s): 兼松泰男, 市田秀樹, 谷川健人, 濱田格雄, 中村亮介
  • Organizer: 日本物理学会2008年秋季大会
  • Place of Presentation: 岩手
  • Year and Date: 2008-09-20
• [Presentation] 整形光パルス列による分子認識 (2008)
  • Author(s): 兼松泰男
  • Organizer: 日本物理学会
  • Place of Presentation: 岩手
  • Year and Date: 2008-09-20
• [Book] Molecular Nano Dynamics(Eds.,H.Fukumura, M.Uosaki, Multidimensional Fluorescence Imaging for Non-invasive Tracking of Cell Responses, vol.2, Chapt 32) (2009)
  • Author(s): R. Nakamura, Y. Kanematsu
  • Publisher: Wiley-VCH
• [Book] "Multidimensional Fluorescence Imaging for Non-invasive Tracking of Cell Responses" in Molecular Nano Dynamics Vol.2 (2009)
  • Author(s): R.Nakamura, Y.Kanematsu
  • Publisher: Wiley-VCH