Research on mechanisms of neural transmission by neuron cell chip and X-ray microbeam irradiation system

• Project/Area Number: 20760047
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied physics, general
• Research Institution: Osaka University
• Principal Investigator: SATO Fuminobu Osaka University, 工学研究科, 助教 (40332746)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 放射線 / 細胞照射 / X線マイクロビーム / パッチクランプ / 放射線照射効果 / 培養細胞パターニング

Research Abstract

A cell chip on which PC12 cells were artificially-arranged was developed by use of techniques of semiconductor device fabrication and biotechnology. Patterned layer for cellular adhesiveness was printed on a glass plate by lithography. After sterilizing the glass plate, the PC12 cells were seeded on the glass plate and cultured in an incubator. The neural differentiation of the PC12 cells was induced by nerve growth factor. Some PC12 cells on the cell chip were irradiated by X-ray microbeam. The radiobiological effects such as damage and repair in the target cell were investigated by immunofluorescence staining and patch clamp methods.

Research Products

• [Journal Article] Development of cell chip based on track detector for examination of biological damage by alpha particles (2010)
  • Author(s): T.Kuchimaru, F.Sato, S.Tanaka, I.Murata, Y.Kato, T.Iida
  • Journal Title: Journal of Nuclear Science Technology 47,12 Pages: 1206-1210
  • NAID: 10027127029
• [Journal Article] Development of Cell Chip Based on Track Detector for Examination of Biological Damage by Alpha Particles (2010)
  • Author(s): Takahiro Kuchimaru, Fuminobu Sato, Souichi Tanaka, Isao Murata, Yushi Kato, Toshiyuki Iida
  • Journal Title: Journal of Nuclear Science and Technology Volume: 47 Pages: 1206-12010
  • NAID: 10027127029
  • Peer Reviewed
• [Journal Article] New Radiophotoluminescence Glass Dosimeter with Specialized Radiation Sensitive Surface Layer (2010)
  • Author(s): Daisuke Maki, Hatsumi Kobayashi, Fuminobu Sato, Isao Murata, Yushi Kato, Takayoshi Yamamoto, Toshiyuki Iida
  • Journal Title: Japanese Journal of Applied Physics Volume: 49 Pages: 116401-116405
  • NAID: 40017377308
  • Peer Reviewed
• [Journal Article] Radiophotoluminescent observation of X-ray microbeam track in silver-activated phosphate glass (2009)
  • Author(s): Y.Aoi, T.Kuchimaru, D.Maki, F.Sato, T.Ikeda, Y.Kato, T.Yamamoto, T.Iida
  • Journal Title: Japanese Journal of Applied Physics 48 Pages: 56001-56001
  • NAID: 40016585529
• [Journal Article] Radiophotoluminescent Observation of X-ray Microbeam Track in Silver-Activated Phosphate Glass (2009)
  • Author(s): Y.Aoi, T.Kuchimaru, D.Maki, F.Sato, T.Ikeda, Y.Kato, T.Yamamoto, T.Iida
  • Journal Title: Japanese Journal of Applied Physics 48 Pages: 560011-560014
  • NAID: 40016585529
  • Peer Reviewed
• [Journal Article] Response of a Radiophotoluminescence Glass Dosimeter to Low Energy X-rays Necessary for Mammography (2009)
  • Author(s): D.Maki, H.Kobayashi, S.Hisakado, F.Sato, Y.Kato, T.Yamamoto, T.Iida
  • Journal Title: Radiation Safety Management 8 Pages: 8-13
  • NAID: 130001477609
  • Peer Reviewed
• [Journal Article] Radiophotoluminescent observation of X-ray microbeam transport in silver-activated phosphate glass (2009)
  • Author(s): Y. Aoi, T. Kuchimaru, D. Maki, F. Sato, T. Ikeda, Y. Kato and T. Iida
  • Journal Title: Japan Journal of Applied Physics (In press)
  • Peer Reviewed
• [Journal Article] Microchamber arrays for the identification of individual cells exposed to an X-ray microbeam (2008)
  • Author(s): T.Kuchimaru, F.Sato, Y.Aoi, T.Fujita, T.Ikeda, K.Shimizu, Y.Kato, T.Iida
  • Journal Title: Radiation and Environmental Biophysics 47 Pages: 535-540
• [Journal Article] Microchamber arrays for the identification of individual cells exposed to an X-ray microbeam (2008)
  • Author(s): T. Kuchimaru, F. Sato, Y. Aoi, T. Fujita, T. Ikeda, K. Shimizu, Y. Kato and T. Iida
  • Journal Title: Radiation and Environmental Biophysics 47 Pages: 535-540
  • Peer Reviewed
• [Journal Article] 単一細胞照射実験のための小型マイクロX線ビーム装置 (2008)
  • Author(s): 青位祐輔、口丸高弘、佐藤文信、加藤裕史、飯田敏行
  • Journal Title: 日本放射線安全管理学会誌 7 Pages: 163-167
  • NAID: 130004175563
  • Peer Reviewed
• [Journal Article] X-ray microbeam measurement with radiophotoluminescent glass plate for single cell irradiation (2008)
  • Author(s): F. Sato, T. Kuchimaru, T. Ikeda, K. Shimizu, Y. Kato, T. Yamamoto, T. Iida
  • Journal Title: Radiation Measurements 43 Pages: 912-916
  • Peer Reviewed
• [Presentation] 単一細胞のX線ビーム照射実験 (2010)
  • Author(s): 田中聡一, 豊田康英, 高橋宏典, 佐藤文信, 清水喜久雄, 加藤裕史, 飯田敏行
  • Organizer: 日本放射線安全管理学会第9回学術大会広島
  • Place of Presentation: 東広島市広島大学東広島キャンパス
  • Year and Date: 2010-12-01
• [Presentation] 単一細胞のX線ビーム照射実験 (2010)
  • Author(s): 田中聡一、豊田康英、高橋宏典、佐藤文信、清水喜久雄、加藤裕史、飯田敏行
  • Organizer: 日本放射線安全管理学会第9回学術大会
  • Place of Presentation: 広島大学
• [Presentation] Development of live cell chips to research effects of X-ray radiation to neuronal cells (2009)
  • Author(s): C.Inagawa, Y.Aoi, T.Kuchimaru, F.Sato, Y.Kato, T.Iida
  • Organizer: 2^<nd> Asian Congress of Radiation Research
  • Place of Presentation: Seoul, Korean
• [Presentation] Development of live Cell Chips to Research Effects of X-ray Radiation to Neuronal Cells (2009)
  • Author(s): C.Inagawa, Y.Aoi, T.Kuchimaru, F.Sato, Y.Kato, T.Iida
  • Organizer: 2^<nd> Asian Congress of Radiation Research
  • Place of Presentation: Seoul, Korean
• [Presentation] X-ray microbeam system for irradiation of living cells (2008)
  • Author(s): T. Kuchimaru, F. Sato, Y. Aoi, Y. Kato and T. Iida
  • Organizer: International Conference on Nuclear Micro-probe Technology and Applications
  • Place of Presentation: Debrecen, Hungary
• [Presentation] Development and testing of micropatterned cell culture platform for biological analysis of single cell irradiation (2008)
  • Author(s): T. Kuchimaru, F. Sato, Y. Aoi, C. Inaaawa, T. Ikeda, Y. Kato and T. Iida
  • Organizer: International Conference on Nuclear Micro-probe Technology and Applications
  • Place of Presentation: Debrecen, Hungary