Research on mechanisms of neural transmission by neuron cell chip and X-ray microbeam irradiation system
| Project/Area Number |
20760047
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied physics, general
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| Research Institution | Osaka University |
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Principal Investigator |
SATO Fuminobu Osaka University, 工学研究科, 助教 (40332746)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 放射線 / 細胞照射 / X線マイクロビーム / パッチクランプ / 放射線照射効果 / 培養細胞パターニング |
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| Research Abstract |
A cell chip on which PC12 cells were artificially-arranged was developed by use of techniques of semiconductor device fabrication and biotechnology. Patterned layer for cellular adhesiveness was printed on a glass plate by lithography. After sterilizing the glass plate, the PC12 cells were seeded on the glass plate and cultured in an incubator. The neural differentiation of the PC12 cells was induced by nerve growth factor. Some PC12 cells on the cell chip were irradiated by X-ray microbeam. The radiobiological effects such as damage and repair in the target cell were investigated by immunofluorescence staining and patch clamp methods.
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Report
(4 results)
Research Products
(17 results)
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[Presentation] 単一細胞のX線ビーム照射実験2010
Author(s)
田中聡一, 豊田康英, 高橋宏典, 佐藤文信, 清水喜久雄, 加藤裕史, 飯田敏行
Organizer
日本放射線安全管理学会第9回学術大会広島
Place of Presentation
東広島市広島大学東広島キャンパス
Year and Date
2010-12-01
Related Report
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