Development of recovery process for proteins utilizing reversible structural change of stimuli-responsive copolymers
| Project/Area Number |
20760512
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
MORISADA Shintaro Tokyo Institute of Technology, 大学院・総合理工学研究科, 助教 (60401569)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 吸着 / 脱着 / 温度スイング / タンパク質 / 感温性高分子 / 高分子ゲル / シリカ粒子 / 高分子修飾 / 刺激応答型高分子 |
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| Research Abstract |
The adsorption and desorption behaviors of bovine serum albumin (BSA) in water for the temperature-responsive adsorbents have been investigated by the temperature-swing operation. As the temperature-responsive adsorbents, the copolymer gels composed of N-isopropylacrylamide (NIPA) and vinylbenzyl trimethylammonium chloride (VBTA) and the poly(NIPA-co-VBTA) grafted silica particles were employed. As a result, it was found that both copolymer adsorbents can adsorb BSA at 298 K and desorb some of pre-adsorbed BSA at 313 K repeatedly by the temperature-swing operation.
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Report
(3 results)
Research Products
(7 results)
