| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
We developed DNA-surfactant as an extractant for sequence-specific DNA extraction in a reverse-micellar system. The DNA-surfactant is a short DNA oligonucleotide conjugated with a hydrophobic oleoyl group. A DNA-surfactant is selectively hybridized to a complementary target DNA to form a double helix. The helix is transferred into a water-pool of a reverse micelle, resulting in the DNA extraction to an organic phase. As a result, we achieved sequence-selective DNA extraction.
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