Sequence-specific separation of functional nucleic acids by liquid-liquid extraction using molecular nano-selfassembly
Project/Area Number |
20760515
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
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Research Institution | Kobe University |
Principal Investigator |
MARUYAMA Tatsuo Kobe University, 大学院・工学研究科, 准教授 (30346811)
|
Project Period (FY) |
2008 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 抽出 / 核酸 / 選択性 / 生産 / 非水系 / 溶媒抽出 / ミセル |
Research Abstract |
We developed DNA-surfactant as an extractant for sequence-specific DNA extraction in a reverse-micellar system. The DNA-surfactant is a short DNA oligonucleotide conjugated with a hydrophobic oleoyl group. A DNA-surfactant is selectively hybridized to a complementary target DNA to form a double helix. The helix is transferred into a water-pool of a reverse micelle, resulting in the DNA extraction to an organic phase. As a result, we achieved sequence-selective DNA extraction.
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Report
(3 results)
Research Products
(5 results)