Development of Gene Expression Analysis Technique from Single-Cells by Using Micro-Tools Filled with Porous Carrier

• Project/Area Number: 20760535
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: ARAKAKI Atsushi Tokyo University of Agriculture and Technology, 大学院・共生科学技術研究院, 助教 (10367154)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 単一細胞解析 / 遺伝子発現解析 / マイクロツール / mRNA 回収 / 単一細胞 / mRNA回収

Research Abstract

In the aim of gene expression analysis from single-cells, micro-tools enabling accurate separation of target single-cells and high efficient mRNA extraction methods were developed. Gene expression and sequence analyses from single immune cells were achieved by the methods using the micro-tools. The developed cell analysis techniques have further potential as a valuable and widely applicable for studies on gene screening and expression analyses of various kinds of cells.

Research Products

• [Journal Article] TCR-Beta Repertoire Analysis of Antigen-Specific Single T-Cells Using a High-Density Microcavity Array (2010)
  • Author(s): Arakaki A, Ooya K, Akiyama Y, Hosokawa M, Komiyama M, Iizuka A, Yamaguchi K, Matsunaga T.
  • Journal Title: Biotechnol Bioeng 106 Pages: 311-318
  • Peer Reviewed
• [Journal Article] TCR-beta repertoire analysis of antigen-specific single T-cells using a high-density microcavity array (2010)
  • Author(s): Arakaki, et al.
  • Journal Title: Biotechnol.Bioeng. 106 Pages: 311-318
  • Peer Reviewed
• [Journal Article] High-Density Microcavity Array for Cell Detection: Single-Cell Analysis of Hematopoietic Stem Cells in Peripheral Blood Mononuclear Cells. (2009)
  • Author(s): Hosokawa M, Arakaki A, Takahashi M, Mori T, Takeyama H, Matsunaga T.
  • Journal Title: Anal Chem 81 Pages: 5308-5313
  • Peer Reviewed
• [Journal Article] High-density microcavity array for cell detection : single-cell analysis of hematopoietic stem cells in peripheral blood mononuclear cells (2009)
  • Author(s): Hosokawa, Arakaki, et al.
  • Journal Title: Anal.Chem. 81 Pages: 5308-5313
  • Peer Reviewed
• [Journal Article] High-Efficiency Single-Cell Entrapment and Fluorescence in Situ Hybridization Analysis Using a Poly(dimethylsiloxane) Microfluidic Device Integrated with a Black Poly(ethylene terephthalate) Micromesh. (2008)
  • Author(s): Matsunaga T, Hosokawa M, Arakaki A, Taguchi T, Mori T, Takeyama H.
  • Journal Title: Anal Chem 80 Pages: 5139-5145
  • Peer Reviewed
• [Journal Article] High-Efficiency Single-Cell Entrapment and Fluorescence in Situ Hybridization Analysis Using a Poly (dimethylsiloxane) Microfluidic Device Integrated with a Black Poly(ethvlene tereuhthalate)Micromesh (2008)
  • Author(s): T. Matsunaga, et.al.
  • Journal Title: Analytical Chemistry 80 Pages: 5139-5145
  • Peer Reviewed
• [Presentation] 全血からの循環腫瘍細胞高効率検出デバイスの開発 (2010)
  • Author(s): 細川正人、早田大志、福田頼謙、新垣篤史、吉野知子、松永是
  • Organizer: 第90春季年会日本化学会
  • Place of Presentation: 近畿大学(大阪府)
  • Year and Date: 2010-03-29
• [Presentation] 全血からの循環腫瘍細胞高効率検出デバイスの開発 (2010)
  • Author(s): 細川正人、新垣篤史, ほか4名
  • Organizer: 第90春季年会 日本化学会
  • Place of Presentation: 近畿大学(大阪府)
  • Year and Date: 2010-03-29
• [Presentation] 高密度単一細胞アレイを用いた希少細胞検出技術の開発 (2009)
  • Author(s): 細川正人、新垣篤史、竹山春子、松永是
  • Organizer: 化学センサ研究会
  • Place of Presentation: 東京農工大学小金井キャンパス(東京都)
  • Year and Date: 2009-09-10
• [Presentation] 高密度単一細胞アレイを用いた希少細胞検出技術の開発 (2009)
  • Author(s): 細川正人、新垣篤史、大森啓太郎、田中あかね、松田浩珍、田中剛、松永是
  • Organizer: (春)電気化学会第76回大会
  • Place of Presentation: 京都大学吉田キャンパス(京都府)
  • Year and Date: 2009-03-31
• [Presentation] 高密度単一細胞アレイを用いた希少細胞検出技術の開発 (2009)
  • Author(s): 細川正人
  • Organizer: (春)電気化学会第76回大会
  • Place of Presentation: 京都大学吉田キャンパス
  • Year and Date: 2009-03-31
• [Book] シングルセル解析の最前線(第4章10 1細胞分離技術を用いたがん特異的免疫細胞の遺伝子スクリーニング法の開発) (2010)
  • Author(s): 秋山靖人、新垣篤史(分担)
  • Publisher: シーエムシー出版
• [Book] シングルセル解析の最前線「第4章10 1細胞分離技術を用いたがん特異的免疫細胞の遺伝子スクリーニング法の開発」 (2010)
  • Author(s): 秋山靖人、新垣篤史
  • Publisher: シーエムシー出版
• [Remarks]
  • URL: http://www.tuat.ac.jp/~matunaga/
• [Patent(Industrial Property Rights)] サイズ選択のマイクロキャビティアレイを用いた循環腫瘍細胞の検出 (2010)
  • Inventor(s): 松永是、新垣篤史、細川正人
  • Industrial Property Rights Holder: 国立大学法人東京農工大学
  • Industrial Property Number: 2010-024774
  • Filing Date: 2010-02-05