Breeding of yeast for efficient bio-ethanol production

• Project/Area Number: 20760538
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Kanazawa University
• Principal Investigator: NINOMIYA Kazuaki Kanazawa University, 環日本海域環境研究センター, 助教 (10379125)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: バイオエタノール / 酵母 / 酵素発現 / FACS / 転写因子 / 代謝進化工学

Research Abstract

By using immunocytochemistry and fluorescent activated cell sorter (FACS), yeast population highly expressing cellulase on the cell surface was enriched from the mutant population prepared by irradiation with carbon ion beams (220 MeV^<12>C^<5+>, 100 Gy). The cellulase activity of selected yeasts was 5.2×10^<-3>U/OD unit, whereas cellulase activity of parental strain was 1.6×10^<-3>U/OD unit.

Research Products

• [Journal Article] Suteaki Shioya Effect of CO2 concentration on the growth and exopolysaccharide production of Bifidobacterium longum cultivated under anaerobic conditions (2009)
  • Author(s): Kazuaki Ninomiya, Kazuhiro Matsuda, Takashi Kawahata, Tadashi Kanaya, Mamiko Kohno, Yoshio Katakura, Masanori Asada
  • Journal Title: J. Biosci. Bioeng. 107(5) Pages: 535-7
  • Peer Reviewed
• [Journal Article] Effect of CO_2 concentration on the growth and exopolysa ccharide production of Bifidobacterium longum cultivated under anaerobic conditions (2009)
  • Author(s): Kazuaki Ninomiva, Kazuhiro Matsuda, Takashi Kawahata, Tadashi Kanaya, Mamiko Kohno, Yoshio Katakura, Masanori Asada, Suteaki Shioya
  • Journal Title: J.Biosci.Bioeng. 107(5) Pages: 535-537
  • Peer Reviewed
• [Journal Article] Biological detoxification of waste house wood hydrolysate using Ureibacillus thermosphaericus for bioethanol production (2008)
  • Author(s): Naoyuki Okuda, Mayumi Soneura, Kazuaki Ninomiya, Yoshio Katakura, S. Shioya
  • Journal Title: J Biosci Bioeng. 106(2) Pages: 128-33
  • NAID: 110006873800
  • Peer Reviewed
• [Journal Article] Strategies for reducing supplemental medium cost in bioethanol production from waste house wood hydrolysate by ethanologenic Escherichia coli: Inoculum size increase and co-culture with Saccharomyces cerevisiae. (2008)
  • Author(s): Naoyuki Okuda, Kazuaki. Ninomiya, Yoshio Katakura, Suteaki Shioya
  • Journal Title: J. Biosci. Bioeng. 105(2) Pages: 90-96
  • NAID: 110006623202
  • Peer Reviewed
• [Journal Article] Biological detoxification of waste house wood hydrolysate using Ureibacillus thermosphaericus for bioethanol production (2008)
  • Author(s): Naoyuki Okuda, Mayumi Soneura, Kazuaki Ninomiya, Yoshio Katakura, S. Shioya
  • Journal Title: J Biosci Bioeng 106(2) Pages: 128-33
  • NAID: 110006873800
  • Peer Reviewed
• [Journal Article] Strategies for reducing supplemental medium cost in bioethanol production from waste house wood hydrolysate by ethanologenic Escherichia coli Inoculum size increase and co-culture with Saccharomvces cerevisiae (2008)
  • Author(s): Naoyuki Okuda, Kazuaki. Ninomiya, Yoshio Katakura, Suteaki Shioya
  • Journal Title: J. Biosci. Bioeng 105(2) Pages: 90-96
  • Peer Reviewed
• [Presentation] ケナフチップの酵素糖化に及ぼす超音波照射処理の効果 (2009)
  • Author(s): 仁宮一章、田中順喜、清水宣明
  • Organizer: 日本ソノケミストリー学会第18回討論会
  • Place of Presentation: 長岡技術科学大学(新潟県)
• [Presentation] 固体連続併行複発酵によるバイオエタノールの生産 (2009)
  • Author(s): 岸本淳平, MOUKAMNERD Churairat, 大道徹太郎, 平尾桂一, 仁宮一章, 塩谷捨明, 紀ノ岡正博, 片倉啓雄
  • Organizer: 日本生物工学会61回大会
  • Place of Presentation: 名古屋大学(愛知県)
• [Presentation] 酵母に表層提示される糖化酵素のみかけの活性に及ぼす糖鎖修飾の影響 (2009)
  • Author(s): 片倉啓雄, 加藤真由, 仁宮一章, 近藤昭彦, 植田充美, 塩谷捨明
  • Organizer: 日本生物工学会61回大会
  • Place of Presentation: 名古屋大学(愛知県)
• [Presentation] Ethanol production from biomass by Consolidated Continuous Solid State Fermentation System (2009)
  • Author(s): M. Churairat, 原田佳苗, 大道徹太郎, 平尾桂一, 片倉啓雄, 仁宮一章, 塩谷捨明
  • Organizer: 化学工学会第74年会
  • Place of Presentation: 横浜国立大学(神奈川県)
• [Presentation] ケナフチップの酵素糖化に及ぼす超音波照射処理の効果 (2009)
  • Author(s): 仁宮一章、田中順喜、清水宣明
  • Organizer: 日本ソノケミストリー学会 第18回討論会
  • Place of Presentation: 長岡技術科学大学(新潟県)
• [Presentation] 固体連続併行複発酵によるバイオエタノールの生産 (2009)
  • Author(s): 岸本淳平, MOUKAMNERD Churairat, 大道徹太郎, 平尾桂一, 仁宮一章, 塩谷捨明, 紀ノ岡正博, 片倉啓雄
  • Organizer: 日本生物工学会 61回大会
  • Place of Presentation: 名古屋大学(愛知県)
• [Presentation] 酵母に表層提示される糖化酵素のみかけの活性に及ぼす糖鎖修飾の影響 (2009)
  • Author(s): 片倉啓雄, 加藤真由, 仁宮一章, 近藤昭彦, 植田充美, 塩谷捨明
  • Organizer: 日本生物工学会 61回大会
  • Place of Presentation: 名古屋大学(愛知県)
• [Presentation] Ethanol production from biomass by Consolidated Continuous Solid State Fermentation System (2009)
  • Author(s): M.Churairat, 原田佳苗, 大道徹太郎, 平尾桂一, 片倉啓雄, 仁宮一章, 塩谷捨明
  • Organizer: 化学工学会第74年会
  • Place of Presentation: 横浜
• [Presentation] Ureibacillus thermosphaericusを用いた木質系バイオマス加水分解液の発酵阻害物質除去 (2008)
  • Author(s): 奥田直之, 埣浦真由美, 仁宮一章, 片倉啓雄, 塩谷捨明
  • Organizer: 日本生物工学会60回大会
  • Place of Presentation: 東北学院大学(宮城県)
  • Year and Date: 2008-08-27
• [Presentation] Ureibacillus thermosphaericusを用いた木質系バイオマス加水分解液の発酵阻害物質除去 (2008)
  • Author(s): 奥田直之, 埣浦真由美, 仁宮一童, 片倉啓雄, 塩谷捨明
  • Organizer: 日本生物工学会60回大会
  • Place of Presentation: 仙台
• [Patent(Industrial Property Rights)] バイオマスを用いたアルコール又は有機酸の製造方法 (2009)
  • Inventor(s): 仁宮一章, 高橋憲司, 清水宣明
  • Industrial Property Rights Holder: 金沢大学
  • Filing Date: 2009-10-16
• [Patent(Industrial Property Rights)] バイオマスを用いたアルコール又は有機酸の製造方法 (2009)
  • Inventor(s): 仁宮一章, 高橋憲司, 清水宣明
  • Industrial Property Rights Holder: 金沢大学
  • Industrial Property Number: 2009-238797
  • Filing Date: 2009-10-16