| Project/Area Number |
20760541
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Fukushima Medical University |
|---|
Principal Investigator |
HIGASHI Tsunehito Fukushima Medical University, 医学部, 助教 (90453018)
|
|---|
| Research Collaborator |
WADA Ikuo 福島県立医科大学, 医学部, 教授 (40182969)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 動物細胞工学 / 小胞体 / 品質管理 / 全反射顕微鏡 |
|---|
| Research Abstract |
We established the method for mobility analyses of cargo proteins in endoplasmic reticulam by total internal reflection fluorescence microscopy. To deal with the large amount of data, we proposed the method using image analyses. Using improved resolution in time and space, we are now able to analyze simple diffusion of cytoplasmic proteins. Finally, we identified temperature stress as one of the factors affected on cargo protein mobility by established methods.
|
