Understanding of cellular function of compatible solutes utilizing a synthetic approach

• Project/Area Number: 20760544
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Konan University
• Principal Investigator: KOUMOTO Kazuya Konan University, フロンティアサイエンス学部・生命化学科, 准教授 (60388759)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 生物機能工学 / 酵素活性 / α-グルコシダーゼ / ベタイン / 適合溶質 / 活性向上

Research Abstract

It is becoming clear that certain metabolites play significant roles in participating in important cellular functions. In order to clarify their cellular function, applicant synthesized metabolite mimics and investigated the functions by mixing them with various biomolecules. As a result, synthetic metabolites possessing certain chemical structures showed profound effect on the stabilization and activation of cellular biomolecules.

Research Products

• [Journal Article] Enhanced amplification of polymerase chain reaction by addition of cosolutes derived from a cellular compatible solute (2008)
  • Author(s): K. Koumoto, H. Ochiai, N. Sugimoto
  • Journal Title: Nucleic Acids Symp. Ser. 52巻 Pages: 257-258
• [Journal Article] Enhanced amplification of polymerase chain reaction by addition of cosolutes derived from a cellular compatible solute (2008)
  • Author(s): K. Koumoto, H. Ochiai, N. Sugimoto
  • Journal Title: Nucleic Acids Symp. Ser 52 Pages: 257-258
• [Presentation] 細胞内代謝産物を基体とする酵素活性化剤 (2010)
  • Author(s): 甲元一也
  • Organizer: 関西6私大新技術説明会
  • Place of Presentation: 東京(口頭発表)
  • Year and Date: 2010-02-26
• [Presentation] 細胞内代謝産物を基体とする酵素活性化剤 (2010)
  • Author(s): 甲元一也
  • Organizer: 関西6私大新技術説明会
  • Place of Presentation: 東京
  • Year and Date: 2010-02-26
• [Presentation] 合成ライブラリーを用いた細胞内代謝産物群が酵素活性に及ぼす影響の解明 (2010)
  • Author(s): 甲元一也、出口瑛介
  • Organizer: 第12回生命化学研究会
  • Place of Presentation: 福井(ポスター発表)
  • Year and Date: 2010-01-08
• [Presentation] 合成ライブラリーを用いた細胞内代謝産物群が酵素活性に及ぼす影響の解明 (2010)
  • Author(s): 甲元一也、出口瑛介
  • Organizer: 第12回生命化学研究会
  • Place of Presentation: 福井
  • Year and Date: 2010-01-08
• [Presentation] 対イオン構造を有する合成代謝産物ライブラリーの創製 (2009)
  • Author(s): 出口瑛介、甲元一也
  • Organizer: 第29回有機合成若手セミナー
  • Place of Presentation: 神戸(ポスター発表)
  • Year and Date: 2009-11-24
• [Presentation] 対イオン構造を有する合成代謝産物ライブラリーの創製 (2009)
  • Author(s): 出口瑛介、甲元一也
  • Organizer: 第29回有機合成若手セミナー
  • Place of Presentation: 神戸
  • Year and Date: 2009-11-24
• [Presentation] Function of cellular metabolites for enzymatic reactions (2009)
  • Author(s): Kazuya Koumoto
  • Organizer: Special Seminar 2 (BK21) in Gyoengsang National University
  • Place of Presentation: Jinju, S. Korea
  • Year and Date: 2009-11-20
• [Presentation] Function of cellular metabolites for enzymatic reactions (2009)
  • Author(s): 甲元一也
  • Organizer: Special Seminar 2(BK21)in Gyoengsang National University
  • Place of Presentation: Jinju, S.Korea
  • Year and Date: 2009-11-20
• [Presentation] Function of cellular metabolites for enzymatic reactions (2009)
  • Author(s): Kazuya Koumoto
  • Organizer: Special Seminar 1 (BK21) in Gyoengsang National University
  • Place of Presentation: Jinju, S. Korea
  • Year and Date: 2009-11-19
• [Presentation] Function of cellular metabolites for enzymatic reactions (2009)
  • Author(s): 甲元一也
  • Organizer: Special Seminar 1(BK21)in Gyoengsang National University
  • Place of Presentation: Jinju, S.Korea
  • Year and Date: 2009-11-19
• [Presentation] 細胞内代謝産物がポリメラーゼ連鎖反応を補助、促進する (2009)
  • Author(s): 甲元一也
  • Organizer: 知財ビジネスマッチングフェア2009
  • Place of Presentation: 大阪(口頭発表)
  • Year and Date: 2009-10-21
• [Presentation] 細胞内代謝産物がポリメラーゼ連鎖反応を補助、促進する (2009)
  • Author(s): 甲元一也
  • Organizer: 知財ビジネスマッチングフエア2009
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-21
• [Presentation] NanoBioNow(22)適合溶質のα-グルコシダーゼ活性に及ぼす影響 (2009)
  • Author(s): 出口瑛介、甲元一也、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋(口頭発表)
  • Year and Date: 2009-03-27
• [Presentation] NanoBioNow(22)適合溶質のα-グルコシダーゼ活性に及ぼす影響 (2009)
  • Author(s): 出口瑛介、甲元一也、杉本直己
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2009-03-27
• [Presentation] 有機合成的手法を用いた代謝産物群のα-グルコシダーゼ活性化メカニズムの解明 (2009)
  • Author(s): 出口瑛介、甲元一也
  • Organizer: 生体機能関連化学若手の会第21回サマースクール
  • Place of Presentation: 京都(ポスター発表)
• [Presentation] 有機合成的手法を用いた代謝産物群のα-グルコシダーゼ活性化メカニズムの解明 (2009)
  • Author(s): 出口瑛介、甲元一也
  • Organizer: 生体機能関連化学若手の会第21回サマースクール
  • Place of Presentation: 京都
• [Presentation] Enhanced amplification of polymerase chain reaction by addition of cosolutes derived from a cellular compatible solute (2008)
  • Author(s): K. Koumoto, H. Ochiai, N. Sugimoto
  • Organizer: Joint Symposium of 18th International Roundtable on Nucleosides, nucleotides and Nucleic Acids and 35th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 京都(ポスター発表)
  • Year and Date: 2008-09-09
• [Presentation] Enhanced amplification of polymerase chain reaction by addition of cosolutes derived from a cellular compatible solute (2008)
  • Author(s): K. Koumoto, H. Ochiai, N. Sugimoto
  • Organizer: Joint Symposium of 18^<th> International Roundtable on Nucleosides, nucleotides and Nucleic Acids an d 35^<th> International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 京都
  • Year and Date: 2008-09-09
• [Presentation] Design of functional additives for the facilitation of polymerase chain reaction (2008)
  • Author(s): K. Koumoto
  • Organizer: FIBER International Symposium on Nucleic Acid Chemistry
  • Place of Presentation: 神戸
  • Year and Date: 2008-09-07
• [Patent(Industrial Property Rights)] 酵素活性を向上させるための組成物およびその利用 (2010)
  • Inventor(s): 甲元一也、出口瑛介
  • Industrial Property Rights Holder: 学校法人甲南学園
  • Industrial Property Number: 2010-001986
  • Filing Date: 2010-01-07
• [Patent(Industrial Property Rights)] 酵素活性を向上させるための組成物およびその利用 (2009)
  • Inventor(s): 甲元一也、出口瑛介
  • Industrial Property Rights Holder: 学校法人甲南学園
  • Industrial Property Number: 2009-046946
  • Filing Date: 2009-02-27