Functions of multi-functional transcription factors in recombination activation

• Project/Area Number: 20770001
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: The University of Tokyo
• Principal Investigator: YAMADA Takatomi The University of Tokyo, 大学院・総合文化研究科, 助教 (30451850)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 減数分裂 / 相同組換え / 多機能性転写因子 / ATF / CREB

Research Abstract

We are interested in mechanisms of homologous recombination in chromatin structure. In this study, we focused on a multi-functional transcription factor Atf1, and examined its molecular features in recombination activation. Our study revealed that Atf1 and its surrounding histones have unique posttranslational modification patterns, and such patterns are in close relationship to recombination activity.

Research Products

• [Journal Article] Analysis of chromatin structure at meiotic DSB sites in yeasts (2009)
  • Author(s): Kouji Hirota, Tomoyuki Fukuda, Takatomi Yamada, Kunihiro Ohta
  • Journal Title: Methods in Molecular Biology vol.557 Pages: 253-66
• [Journal Article] Analysis of chromatin structure at meiotic DSB sites in yeasts (2009)
  • Author(s): Kouji Hirota, Tomoyuki Fukuda, Takatomi Yamada, Kunihiro Ohta
  • Journal Title: Methods in Molecular Biology 557 Pages: 253-266
• [Journal Article] Molecular characterization of the role of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double strand break repair in association with the Mre11-Rad50-Nbs1 complex (2008)
  • Author(s): ufuko Akamatsu, Yasuto Murayama, Takatomi Yamada, Tomofumi Nakazaki, Yasuhiro Tsutsui, Kunihiro Ohta, Hiroshi Iwasaki
  • Journal Title: Molecular and Cellular Biology vol.28 Pages: 3639-3651
• [Presentation] Histone modifications around ATF/CREB-dependent meiotic recombination hotspots (2009)
  • Author(s): Shintaro Yamada, Takatomi Yamada, Kunihiro Ohta
  • Organizer: The Fifth International Fission Yeast Meeting
  • Place of Presentation: 東京
  • Year and Date: 2009-10-28
• [Presentation] Residue specific actylations around ATF/CREB-dependent recombination hotspots in fission yeast (2009)
  • Author(s): Takatomi Yamada, Shintaro Yamada, Kunihiro Ohta
  • Organizer: FASEB Summer Research Conferences, Genetic recombination and genome rearrangements
  • Place of Presentation: Snowmass, Colorado, U.S.A.
  • Year and Date: 2009-08-05
• [Presentation] 分裂酵母ATF/CREBファミリータンパク質Atf21の機能解析 (2009)
  • Author(s): 山田貴富森田智彦太田邦史
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Histone modifications around ATF/CREB-dependent meiotic recombination hotspots (2009)
  • Author(s): Shintaro Yamada, Takatomi Yamada, Kunihiro Ohta
  • Organizer: The Fifth International Fission Yeast Meeting
  • Place of Presentation: Tokyo, Japan
• [Presentation] Residue specific actylations around ATF/CREB-dependent recombination hotspots in fission yeast (2009)
  • Author(s): Takatomi Yamada, Shintaro Yamada, Kunihiro Ohta
  • Organizer: FASEB Summer Research Conferences, Genetic recombination and genome rearrangements
  • Place of Presentation: Colorado U.S.A.
• [Presentation] ATF/CREBファミリータンパク質による減数分裂諸現象の総合的制御 (2008)
  • Author(s): 山田貴富森田智彦太田邦史
  • Organizer: 第31回日本分子生物学会第81回日本生化学会合同大会
  • Place of Presentation: 神戸
• [Presentation] 分裂酵母ATF/CREBファミリータンパク質Atf21の機能解析 (2008)
  • Author(s): 森田智彦山田貴富(発表者)松本幸次太田邦史
  • Organizer: 第41回酵母遺伝学フォーラム
  • Place of Presentation: 札幌
• [Book] 基礎生命科学実験第2版 (2009)
  • Author(s): 東京大学教養学部基礎生命科学実験編集委員会
  • Total Pages: 205
  • Publisher: 東京大学出版会
• [Book] 基礎生命科学実験第2版
  • Author(s): 東京大学教養学部基礎生命科学実験編集委員会(研究代表者を含む)
  • Total Pages: 205
  • Publisher: 東京大学出版会