Novel temperature sensing mechanism through G protein-coupled signaling

• Project/Area Number: 20770003
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Nagoya University
• Principal Investigator: KUHARA Atsushi Nagoya University, 大学院・理学研究科, 助教 (00402412)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: S / 温度走性 / 神経回路 / Gタンパク質 / カルシウムイメージング / 線虫 / 温度受容 / 抑圧変異 / C. elegans / ニューロン

Research Abstract

Temperature is an unavoidable environmental cue that affects metabolism and behavior of any creature on earth. While light and smell are received by G protein-coupled receptors, temperature is received by TRP channel. Here we found G protein-coupled temperature sensing in nematode C.elegans.

Research Products

• [Journal Article] 線虫(C. elegans)の行動を制御する神経回路における嗅覚ニューロンによる温度の感知 (2009)
  • Author(s): 森郁恵, 久原篤
  • Journal Title: サイエンス誌に載った日本人研究者2008 Pages: 20-20
• [Journal Article] 線虫(C.elegans)の行動を制御する神経回路における嗅覚ニューロンによる温度の感知 (2009)
  • Author(s): 森郁恵, 久原篤
  • Journal Title: サイエンス誌に載った日本人研究者2008 Pages: 20-20
• [Journal Article] Temperature sensing by an olfactory neuron in a circuit controlling behavior of C. elegans (2008)
  • Author(s): Kuhara, A., Okumura M., Kimata T., Tanizawa Y., Takano R., Kimura D.K., Inada H., Matsumoto K., Mori I.
  • Journal Title: Science 320 Pages: 803-807
  • Peer Reviewed
• [Journal Article] 神経回路の活動と接続のライブイメージング (2008)
  • Author(s): 久原篤, 木全翼, 森郁恵
  • Journal Title: 実験医学増刊号ライブイメージングで解き明かす多彩な生命現象 Vol.26No.17 Pages: 89-95
  • Peer Reviewed
• [Journal Article] 線虫におけるGタンパクを介した温度感知行動 (2008)
  • Author(s): 久原篤, 森郁恵
  • Journal Title: 細胞工学特集号行動を支配する遺伝子 Vol.27No.11 Pages: 1106-1111
  • Peer Reviewed
• [Journal Article] 線虫C. elegansの温度走性をもちいた感覚と学習行動の遺伝学的研究 (2008)
  • Author(s): 久原篤, 森郁恵
  • Journal Title: 生物物理 48(3) Pages: 167-173
  • Peer Reviewed
• [Journal Article] 温度学習行動の分子神経回路メカニズム (2008)
  • Author(s): 久原篤, 笹倉寛之, 木全翼, 森郁恵
  • Journal Title: 蛋白質核酸酵素増刊号神経の分化, 回路形成, 機能発現(三品昌美・山森哲雄・狩野方伸・村上富士夫・貝渕弘三) Vol.53No.4 Pages: 580-586
• [Journal Article] Temperature sensing by an olfactory neuron in a circuit controlling behavior of C. elegans (2008)
  • Author(s): Kuhara, A.*, Okumura, M.*, Kimata, T., Tanizawa, Y., Takano, R., Kimura, K. D., Inada, H., Matsumoto, K., and Mori I. (*equally contributed)
  • Journal Title: Science 320 Pages: 803-807
  • Peer Reviewed
• [Journal Article] 神経回路の活動と接続のライブイメージング (2008)
  • Author(s): 久原篤, 木全翼, 森郁恵
  • Journal Title: 実験医学増刊号ライブイメージングで解き明かす多彩な生命現象 26(17) Pages: 89-95
  • Peer Reviewed
• [Journal Article] 線虫に置けるGタンパクを介した温度感知行動 (2008)
  • Author(s): 久原篤, 森郁恵
  • Journal Title: 細胞工学特集号行動を支配する遺伝子 27(11) Pages: 1106-1111
  • Peer Reviewed
• [Journal Article] 温度学習行動の分子神経回路メカニズム (2008)
  • Author(s): 久原篤, 笹倉寛之, 木全翼, 森郁恵
  • Journal Title: 蛋白質核酸酵素増刊号神経の分化, 回路形成, 機能発現 53 Pages: 580-586
  • Peer Reviewed
• [Presentation] 神経活動の定量的光操作から見えてきた神経の暗号 (2010)
  • Author(s): 久原篤, 森郁恵
  • Organizer: 定量生物学の会第二回年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-01-11
• [Presentation] 線虫をつかって脳の仕組みを探る (2009)
  • Author(s): 久原篤
  • Organizer: あいち科学技術教育推進協議会発表会「科学三昧inあいち2009」
  • Place of Presentation: 岡崎
  • Year and Date: 2009-12-24
• [Presentation] Neural code underlying inhibitory and excitatory neurotransmissions in single sensory neuron controlling temperature-sensing behavior of C. elegans行動の逆転に関わる単一感覚ニューロンの興奮性と抑制性のシナプス伝達 (2009)
  • Author(s): 久原篤、下和田智康、大西憲幸、森郁恵
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Neural code underlying inhibitory and excitatory neurotransmissions in single sensory neuron controlling temperature-sensing behavior of C.elegans行動の逆転に関わる単一感覚ニューロンの興奮性と抑制性のシナプス伝達 (2009)
  • Author(s): 久原篤、下和田智康、大西憲幸、森郁恵
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Inhibitory and excitatory regulations of the simple neural circuit generate opposite navigation behaviors (2009)
  • Author(s): Atsushi Kuhara, Tomoyasu Shimowada, Ikue Mori
  • Organizer: Neuro 2009
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-19
• [Presentation] 温度受容と記憶学習の神経遺伝学(2009年度日本遺伝学会奨励賞受賞講演) (2009)
  • Author(s): 久原篤
  • Organizer: 日本遺伝学会第81回大会
  • Place of Presentation: 長野
  • Year and Date: 2009-09-17
• [Presentation] Neural code underlying excitatory and inhibitory synaptlc transmlsslon in temperature-sensing neuron essential for temperature memory-governed behavior (2009)
  • Author(s): Atsushi Kuhara, Tomoyasu Shimowada, Noriyuki Ohnishi, Ikue Mori
  • Organizer: 第4回MCCS-Asiaシンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-15
• [Presentation] Neural code underlying excitatory and inhibitory synaptic transmission in temperature-sensing neuron essential for temperature memory-governed behavior (2009)
  • Author(s): Atsushi Kuhara, Tomoyasu Shimowada, Noriyuki Ohnishi, Ikue Mori
  • Organizer: 第4回MCCS-Asiaシンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-15
• [Presentation] 特定のグルタミン酸シナプス伝達を制御する神経の暗号 (2009)
  • Author(s): 久原篤, 森郁恵
  • Organizer: 生理研研究会「光を用いた神経活動の操作-操作法開発から神経回路研究への応用-
  • Place of Presentation: 岡崎
  • Year and Date: 2009-09-04
• [Presentation] 特定のグルタミン酸シナプス伝達を制御する神経の暗号 (2009)
  • Author(s): 久原篤, 森郁恵
  • Organizer: 生理研研究会「光を用いた神経活動の操作-操作法開発から神経回路研究への応用-」
  • Place of Presentation: 岡崎
  • Year and Date: 2009-09-04
• [Presentation] 温度感知行動を司る神経回路システム～分子生理学者が目指すシステムズバイオロジー～ (2009)
  • Author(s): 久原篤, 森郁恵
  • Organizer: 生理研研究会「神経科学の新しい解析法とその応用」
  • Place of Presentation: 岡崎
  • Year and Date: 2009-07-17
• [Presentation] Exploring the neural code in the neural circuit for thermotaxis behavior (2009)
  • Author(s): Atsushi Kuhara, Tomoyasu Shimowada, Noriyuki Ohnishi, Ikue Mori
  • Organizer: 17th International C. elegans Meeting
  • Place of Presentation: Los. Angels
  • Year and Date: 2009-06-26
• [Presentation] Neural Circuits and Behavior in C. elegans II: Towards the Ultimate Model (2009)
  • Author(s): Atsushi Kuhara, Tomoyasu Shimowada, Noriyuki Ohnishi, IkueMori
  • Organizer: Exploring the neural code in thermosensory neural circuit
  • Place of Presentation: Virginia
• [Presentation] Exploring the neural code in thermosensory neural circuit (2009)
  • Author(s): Atsushi Kuhara, Tomoyasu Shimowada, Noriyuki Ohnishi & Ikue Mori
  • Organizer: rence "Neural Circuits and Behavior in C. elegans II : Towards the Ultimate Model"
  • Place of Presentation: Virginia, USA
• [Presentation] G protein-coupled temperature sensing by an olfactory neuron in C. elegans (2008)
  • Author(s): Atsushi Kuhara, Masatoshi Okumura, Ayako Okazaki, Sayaka Tachikawa, Ikue Mori
  • Organizer: Neuro 2008
  • Place of Presentation: 東京
• [Presentation] Integrative analysis on G protein-coupled temperature sensation in C. elegans Gordon Research Conference (2008)
  • Author(s): Atsushi Kuhara, Masatoshi Okumura, Ikue Mori
  • Organizer: Gordon Research Conference "Molecular and Cellular Neurobiology"
  • Place of Presentation: Hong Kong
• [Presentation] Integrative analysis on G protein-coupled temperature sensation in C. elegans Gordon Research Conference (2008)
  • Author(s): Atsushi Kuhara, Masatoshi Okumura, and Ikue Mori
  • Organizer: Gordon Research Conference "Molecular and Cellular Neurobiology"
  • Place of Presentation: Hong Kong, China
• [Book] 嗅覚ニューロンにおけるGタンパクを介した温度受容メカニズム (2009)
  • Author(s): 久原篤
  • Publisher: クバプロ書籍
• [Book] ブレインサイエンスレビュー2009 (2009)
  • Author(s): 久原篤
  • Publisher: 嗅覚ニューロンにおけるGタンパクを介した温度受容メカニズム
• [Remarks]
  • URL: http://elegans.bio.nagoya-u.ac.jp/~kuhara/index.html
• [Remarks]
  • URL: http://elegans.bio.nagoya-u.ac.jp/~kuhara/index.html