Investigation of the interplay among enzymes involved in sulfur assimilation in plants.

• Project/Area Number: 20770027
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Chiba University
• Principal Investigator: YOSHIMOTO Naoko Chiba University, 大学院・薬学研究院, 助教 (10415333)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 植物 / 硫黄代謝産物 / 生合成 / 蛋白質間相互作用 / メタボロミクス / フォーカスドプロテオミクス / 硫黄代謝

Research Abstract

Plants are able to take up sulfate from the soil solution and convert it into sulfur-containing amino acid cysteine. The completion of the Arabidopsis genome project enabled identification of genes potentially encoding enzymes involved in sulfur assimilation. In this project, we have analyzed physiological functions of sulfur assimilatory enzymes. We have also analyzed the interplay among these enzymes.

Research Products

• [Journal Article] Interplay of SLIM1 and mir395 in the regulation of sulfate assimilation in Arabidopsis. (2011)
  • Author(s): Cintia G.Kawashima, Colette A.Matthewman, Siqi Huang, Bok-Rye Lee, Naoko Yoshimoto, Anna Koprivova, Ignacio Rubio-Somoza, Marco Todesco, Tina Rathjen, Kazuki Saito, Hideki Takahashi, Tamas Dalmay, Stanislav Kopriva
  • Journal Title: Plant J.
  • Peer Reviewed
• [Journal Article] Interplay of SLIM1 and miR395 in regulation of sulfate assimilation in Arabidopsis. (2011)
  • Author(s): Cintia G.Kawashima
  • Journal Title: The Plant Journal
  • Peer Reviewed
• [Journal Article] Disruption of adenosine-5'-phosphosulfate kinase in Arabidopsis reduces levels of sulfate secondary metabolites. (2009)
  • Author(s): Sarah G.Mugford, Naoko Yoshimoto, Michael Reichelt, Markus Wirtz, Lionel Hill, Sam T.Mugford, Yoshimi Nakazato, Masaaki Noji, Hideki Takahashi, Robert Kramell, Tamara Gigolashvili, Ulf-Ingo Flugge, Claus Wasternack, Jonathan Gershenzon, Rudiger Hell, Kazuki Saito, Stanislav Kopriva
  • Journal Title: Plant Cell 21 Pages: 910-927
  • Peer Reviewed
• [Journal Article] Sulphur starvation induces the expression of microRNA-395 and one of its target genes but in different cell types. (2009)
  • Author(s): Cintia Goulart Kawashima, Naoko Yoshimoto, Akiko Maruyama-Nakashita, Yumiko N.Tsuchiya, Kazuki Saito, Hideki Takahashi, Tamas Dalmay
  • Journal Title: Plant J. 57 Pages: 313-321
  • Peer Reviewed
• [Journal Article] Post-transcriptional control of high-affinity sulfate transporters for uptake of sulfate in roots of Arabidopsis thaliana (2009)
  • Author(s): Naoko Yoshimoto
  • Journal Title: Sulfur Metabolism in Plants Pages: 179-184
  • Peer Reviewed
• [Journal Article] Characterization of the APS kinase gene family in Arabidopsis thaliana (2009)
  • Author(s): Sarah G.Mugford
  • Journal Title: Sulfur Metabolism in Plants Pages: 189-194
  • Peer Reviewed
• [Journal Article] Cysteine biosynthesis in Arabidopsis : comprehensive reverse genetic study on the functions of BSAS and SERAT gene families (2009)
  • Author(s): Mutsumi Watanabe
  • Journal Title: Sulfur Metabolism in Plants Pages: 201-206
  • Peer Reviewed
• [Journal Article] Sulphur starvation induces the expression of microRNA-395 and one of its target genes but in different cell types (2009)
  • Author(s): Cintia Goulart Kawashima
  • Journal Title: The Plant Journal 57 Pages: 313-321
  • Peer Reviewed
• [Journal Article] Disruption of adenosine-5'-phosphosulfate kinase in Arabidopsis reduces levels of sulfated secondary metabolites (2009)
  • Author(s): Sarah G. Mugford
  • Journal Title: The Plant Cell (in press)
  • Peer Reviewed
• [Journal Article] Comparative genomics and reverse genetics analysis reveal indispensable functions of the serine acetyltransferase gene family in Arabidopsis. (2008)
  • Author(s): Mutsumi Watanabe, Keiichi Mochida, Tomohiko Kato, Satoshi Tabata, Naoko Yoshimoto, Masaaki Noji, Kazuki Saito
  • Journal Title: Plant Cell 20 Pages: 2484-2496
  • Peer Reviewed
• [Journal Article] Comparative genomics and reverse genetics analysis reveal indispensable functions of the serine acetyltransferase gene family in Arabidopsis (2008)
  • Author(s): Mutsumi Watanabe
  • Journal Title: The Plant Cell 20 Pages: 2484-2496
  • Peer Reviewed
• [Presentation] 硫黄同化に関与するATPスルフリラーゼ遺伝子は転写開始点制御により葉緑体型と細胞質型の酵素をコードする (2011)
  • Author(s): 吉本尚子, 関口愛, 高橋秀樹, 斉藤和季
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-22
• [Presentation] 硫黄同化に関与するATPスルフリラーゼ遺伝子は転写開始点制御により葉緑体型と細胞質型の酵素をコードする (2011)
  • Author(s): 吉本尚子
  • Organizer: 第52回 日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-22
• [Presentation] Dualtargeting of Arabidopsis ATP sulfurylase 2 to chloroplast and cytosol by use of multiple in-frame initiation codons. (2010)
  • Author(s): Naoko Yoshimoto, Ai Sekiguchi, Hideki Takahashi, Kazuki Saito
  • Organizer: 8th International Workshop on Sulfur Metabolism in Higher Plants
  • Place of Presentation: Creswick, Australia
  • Year and Date: 2010-11-25
• [Presentation] Dual targeting of Arabidopsis ATP sulfurylase 2 to chloroplast and cytosol by use of multiple in-frame initiation codons. (2010)
  • Author(s): Naoko Yoshimoto
  • Organizer: 8th International Workshop on Sulfur Metabolism in Higher Plants
  • Place of Presentation: オーストラリア・クレスヴイック
  • Year and Date: 2010-11-25
• [Presentation] Partitioning of sulfur between primary and secondary metabolism. (2010)
  • Author(s): Sarah G.Mugford, Colette Matthewman, Bok-Rye Lee, Ruslan Yatusevich, Naoko Yoshimoto, Markus Wirtz, Lionel Hill, Ruediger Hell, Hideki Takahashi, Kazuki Saito, Tamala Gigolashvili, Stanislav Kopriva
  • Organizer: 8th International Workshop on Sulfur Metabolism in Higher Plants.
  • Place of Presentation: Creswick, Australia
  • Year and Date: 2010-11-23
• [Presentation] 硫黄同化系酵素ATPスルフリラーゼ過剰発現植物の解析 (2010)
  • Author(s): 吉本尚子, 土屋(中村)有美子, 高橋秀樹, 斉藤和季
  • Organizer: 第28回日本植物細胞分子生物学会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-03
• [Presentation] シロイヌナズナの硫黄同化に関わるATPスルフリラーゼ群の機能解析 (2010)
  • Author(s): 吉本尚子, 東泰弘, 水野新也, 渡辺むつみ, 高橋秀樹, 野路征昭, 斉藤和季
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-19
• [Presentation] シロイヌナズナの硫黄同化に関わるATPスルフリラーゼ群の機能解析 (2010)
  • Author(s): 吉本尚子
  • Organizer: 第51回 日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-19
• [Presentation] Functional characterization of ATP sulfurylase gene family in Arabidopsis thaliana. (2009)
  • Author(s): Naoko Yoshimoto, Yasuhiro Higashi, Sakiko Katsunuma, Shin'ya Mizuno, Hideki Takahashi, Masaaki Noji, Kazuki Saito
  • Organizer: 2nd SULPHYTON Meeting on Plant Sulfur Research.
  • Place of Presentation: Norwich, UK
  • Year and Date: 2009-09-13
• [Presentation] Functional characterization of ATP sulfurylase gene family in Arabidopsis thaliana (2009)
  • Author(s): 吉本尚子
  • Organizer: 2nd SULPHYTON Meeting on Plant Sulfur Research
  • Place of Presentation: イギリス・ノーリッチ
  • Year and Date: 2009-09-13
• [Presentation] シロイヌナズナにおける硫黄同化系酵素ATPスルフリラーゼの機能解析 (2009)
  • Author(s): 吉本尚子, 東泰弘, 勝沼咲子, 水野新也, 高橋秀樹, 野路征昭, 斉藤和季
  • Organizer: 第27回日本植物細胞分子生物学会
  • Place of Presentation: 藤沢
  • Year and Date: 2009-07-31
• [Presentation] シロイヌナズナの硫黄同化系酵素ATPスルフリラーゼの機能解析 (2009)
  • Author(s): 吉本尚子, 東泰弘, 勝沼咲子, 水野新也, 高橋秀樹, 野路征昭, 斉藤和季
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] シロイヌナズナにおける硫酸化代謝物の合成に関わるAPSキナーゼ群の機能解析 (2008)
  • Author(s): 吉本尚子, 中里好美, 高橋秀樹, 野路征昭, 斉藤和季
  • Organizer: 第26回日本植物細胞分子生物学会
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-02
• [Presentation] Post-transcriptional control of high-affinity sulfate transporters for uptake of sulfate in Arabidopsis roots. (2008)
  • Author(s): Naoko Yoshimoto, Eri Inoue, Akiko Watanabe-Takahashi, Kazuki Saito, Hideki Takahashi
  • Organizer: 7th Workshop on Sulfur in Plants.
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2008-05-16
• [Presentation] Cysteine biosynthesis in Arabidopsis : comprehensive study on the functions of Serat and Bsas gene families. (2008)
  • Author(s): Mutsumi Watanabe, Miyako Kusano, Akira Oikawa, Atsushi Fukushima, Keiichi Mochida, Tomohiko Kato, Satoshi Tabata, Masaaki Noji, Naoko Yoshimoto, Kazuki Saito
  • Organizer: 7th Workshop on Sulfur in Plants.
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2008-05-16
• [Presentation] Jonathan Gershenzon, Stanislav Kopriva : Characterization of the APS kinase gene family in Arabidopsis thaliana. (2008)
  • Author(s): Sarah G.Mugford, Michael Reichelt, Naoko Yoshimoto, Yoshimi Nakazato, Masaaki Noji, Hideki Takahashi, Kazuki Saito, Jonathan Gershenzon, Stanislav Kopriva
  • Organizer: 7th Workshop on Sulfur in Plants.
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2008-05-14
• [Book] Sulfur Metabolism in Plants(Post-transcriptional control of high-affinity sulfate transporters for uptake of sulfate in roots of Arabidopsis thaliana.)(A.Sirko, L.J.De Kok, S.Haneklaus, M.J.Hawkesford, H.Rennenberg, K.Saito, E.Schnug, I.Stulen, eds:) (2009)
  • Author(s): Naoko Yoshimoto, Eri Inoue, Akiko Watanabe-Takahashi, Kazuki Saito, Hideki Takahashi
  • Publisher: Buckhuys Publishers, Leiden
• [Book] Sulfur Metabolism in Plants(Characterization of the APS kinase gene family in Arabidopsis thaliana.)(A.Sirko, L.J.De Kok, S.Haneklaus, M.J.Hawkesford, H.Rennenberg, K.Saito, E.Schnug, I.Stulen, eds:) (2009)
  • Author(s): Sarah G.Mugford, Michael Reichelt, Naoko Yoshimoto, Yoshimi Nakazato, Masaaki Noji, Hideki Takahashi, Kazuki Saito, Jonathan Gershenzon, Stanislav Kopriva
  • Publisher: Buckhuys Publishers, Leiden
• [Book] Sulfur Metabolism in Plants(Cysteine biosynthesis in Arabidopsis : comprehensive reverse genetic study on the functions of BSAS and SERAT gene families.)(A.Sirko, L.J.De Kok, S.Haneklaus, M.J.Hawkesford, H.Rennenberg, K.Saito, E.Schnug, I.Stulen, eds:) (2009)
  • Author(s): Mutsumi Watanabe, Miyako Kusano, Akira Oikawa, Atsushi Fukushima, Keiichi Mochida, Tomohiko Kato, Satoshi Tabata, Naoko Yoshimoto, Masaaki Noji, Kazuki Saito
  • Publisher: Buckhuys Publishers, Leiden