Organellar dynamics in plant male gametophyte visualized by fluorescent live imaging

• Project/Area Number: 20770036
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Okayama University
• Principal Investigator: MATSUSHIMA Ryo Okayama University, 資源生物科学研究所, 助教 (80403476)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: オルガネラ / 生殖 / 蛍光タンパク質 / ライブイメージング解析 / 花粉 / ミトコンドリア / 蛍光蛋白質 / 可視化 / シロイヌナズナ / 受精 / ライブイメージング

Research Abstract

Combination of mitochondria-targeted fluorescent proteins with vegetative or sperm cell-specific expression allowed us to observe precise number and dynamic behavior of mitochondria in the respective cell types. Furthermore, live imaging analysis showed that sperm mitochondria enter into egg and central cells. We showed that the transgenic materials constructed in this project are useful for not only live imaging but also studying mitochondrial functions by mutant analysis.

Research Products

• [Journal Article] GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 are required for maintenance of endoplasmic reticulum morphology in Arabidopsis thaliana. (2009)
  • Author(s): Nakano, R.T., Matsushima, R., Ueda, H., Tamura, K., Shimada, T., Li, L., Hayashi, Y., Kondo, M., Nishimura. M., Hara-Nishimura, I.
  • Journal Title: Plant Cell Vol.21 Pages: 3672-3685
  • Peer Reviewed
• [Journal Article] Visualization of plastids in pollen grains: Involvement of FtsZ1 in pollen plastid division. (2009)
  • Author(s): Tang, L.Y., Nagata, N., Matsushima, R., Chen, Y., Yoshioka, Y.. Sakamoto, W.
  • Journal Title: Plant and Cell Physiology Vol.50 Pages: 904-908
  • NAID: 10027339958
  • Peer Reviewed
• [Journal Article] Visualization of plastids in pollen grains : Involvement of FtsZl in pollen plastid division (2009)
  • Author(s): Tang, L.Y., Nagata, N., Matsushima, R., Chen, Y., Yoshioka, Y., Sakamoto, W.
  • Journal Title: Plant and Cell Physiology 50 Pages: 904908-904908
  • Peer Reviewed
• [Journal Article] GNOM-LIKEl/ERMOl and SEC24a/ERMO2 Are Required for Maintenance of Endoplasmic Reticulum Morphology in Arabidopsis thaliana (2009)
  • Author(s): Nakano, R.T., Matsushima, R., Ueda, H., Tamura, K., Shimada, T., Li, L., Hayashi, Y., Kondo, M., Nishimura, M.
  • Journal Title: Plant Cell 21 Pages: 36723685-36723685
  • Peer Reviewed
• [Journal Article] Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging. (2008)
  • Author(s): Matsushima, R., Hamamura, Y., Higashiyama, T., Arimura, S. -i., Sodmergen, Tsutsumi, N., Sakamoto, W.
  • Journal Title: Plant and Cell Physiology Vol.49 Pages: 1074-1083
  • NAID: 10027332435
  • Peer Reviewed
• [Journal Article] The model plant Medicago truncatula exhibits biparental plastid inheritance. (2008)
  • Author(s): Matsushima, R., Hu, Y., Toyoda, K., Sodmergen, Sakamoto, W.
  • Journal Title: Plant and Cell Physiology Vol.49 Pages: 81-91
  • NAID: 10027495231
  • Peer Reviewed
• [Journal Article] The model plant Medicago truncatula exhibits biparental plastid inheritance (2008)
  • Author(s): Matsushima, R, Hu, Y., Toyoda. K, Sodmergen. Sakamoto, W.
  • Journal Title: Plant and Cell Physiology 49 Pages: 81-91
  • NAID: 10027495231
  • Peer Reviewed
• [Journal Article] Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging (2008)
  • Author(s): Matsushima, R., Hamamura, Y., Higashiyama, T., Arimura. Sodmergen. Tsutsumi. N., Sakamoto, W.
  • Journal Title: Plant and Cell Physiology 49 Pages: 1074-1083
  • NAID: 10027332435
  • Peer Reviewed
• [Presentation] デンプン粒の形状多様性を支配する分子機構の解明にむけて. (2009)
  • Author(s): 松島良・前川雅彦・藤田直子・山下純・坂本亘
  • Organizer: 植物細胞生物学若手の会
  • Place of Presentation: 奈良
• [Presentation] 植物の雄性配偶体形成におけるオルガネラDNAのダイナミクス. (2009)
  • Author(s): 坂本亘・松島良
  • Organizer: 第81回日本遺伝学会
  • Place of Presentation: 松本
• [Presentation] 小胞体の形態と細胞内分布に異常を示すendoplasmic reticulum morphology (ermo)変異体の解析 (2009)
  • Author(s): 中野亮平・松島良・上田晴子・林八寿子・田村謙太郎・嶋田知生・西村いくこ
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] 花粉においてオルガネラDNAの分解を担う遺伝子の単離と解析. (2009)
  • Author(s): 松島良・Lay Yin Tang・蘇都莫日根・David Twell・坂本亘
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] endoplasmic reticulum morphology (ermo) mutants of Arabidopsis thaliana develop a number of novel ER-derived structures in the cells. (2008)
  • Author(s): Nakano, T.R., Matsushima, R., Ueda, H., Hayashi, Y., Tamura, K., Shimada, T., Hara-Nishimura, I.
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同学会
  • Place of Presentation: 神戸
• [Presentation] 花粉における細胞質ゲノムの分解機構に関する研究. (2008)
  • Author(s): 松島良・坂本亘
  • Organizer: 国立遺伝学研究所研究集会「高等植物の生殖過程を制御する因子と生殖隔離」
  • Place of Presentation: 三島
• [Presentation] Mitochondrial dynamics in plant male gametophyte studied by fluorescent live imaging. (2008)
  • Author(s): Matsushima, R., Hamamura, Y., Higashiyama, T., Sakamoto, W.
  • Organizer: Frontiers of Sexual Plant Reproduction III., USA
  • Place of Presentation: Arizona
• [Presentation] Pollen organellar DNA retention in ribonucleotide reductase mutants of Arabidopsis. (2008)
  • Author(s): Tang, L.Y., Matsushima, R., Sakamoto, W.
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
• [Presentation] Mitochondrial dynamics in plant male gametophyte studied by fluorescent live imaging (2008)
  • Author(s): Matsushima, R., Hamamura, Y., Higashiyama, T. and Sakamoto, W.
  • Organizer: Frontiers of Sexual Plant Reproduction III.
  • Place of Presentation: Tucson, Arizona, USA
• [Remarks]
  • URL: http://www.rib.okayama-u.ac.jp/saka/index-j.html