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Organellar dynamics in plant male gametophyte visualized by fluorescent live imaging

Research Project

Project/Area Number 20770036
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionOkayama University

Principal Investigator

MATSUSHIMA Ryo  Okayama University, 資源生物科学研究所, 助教 (80403476)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsオルガネラ / 生殖 / 蛍光タンパク質 / ライブイメージング解析 / 花粉 / ミトコンドリア / 蛍光蛋白質 / 可視化 / シロイヌナズナ / 受精 / ライブイメージング
Research Abstract

Combination of mitochondria-targeted fluorescent proteins with vegetative or sperm cell-specific expression allowed us to observe precise number and dynamic behavior of mitochondria in the respective cell types. Furthermore, live imaging analysis showed that sperm mitochondria enter into egg and central cells. We showed that the transgenic materials constructed in this project are useful for not only live imaging but also studying mitochondrial functions by mutant analysis.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (18 results)

All 2009 2008 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (9 results) Remarks (1 results)

  • [Journal Article] GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 are required for maintenance of endoplasmic reticulum morphology in Arabidopsis thaliana.2009

    • Author(s)
      Nakano, R.T., Matsushima, R., Ueda, H., Tamura, K., Shimada, T., Li, L., Hayashi, Y., Kondo, M., Nishimura. M., Hara-Nishimura, I.
    • Journal Title

      Plant Cell Vol.21

      Pages: 3672-3685

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of plastids in pollen grains: Involvement of FtsZ1 in pollen plastid division.2009

    • Author(s)
      Tang, L.Y., Nagata, N., Matsushima, R., Chen, Y., Yoshioka, Y.. Sakamoto, W.
    • Journal Title

      Plant and Cell Physiology Vol.50

      Pages: 904-908

    • NAID

      10027339958

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of plastids in pollen grains : Involvement of FtsZl in pollen plastid division2009

    • Author(s)
      Tang, L.Y., Nagata, N., Matsushima, R., Chen, Y., Yoshioka, Y., Sakamoto, W.
    • Journal Title

      Plant and Cell Physiology 50

      Pages: 904908-904908

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GNOM-LIKEl/ERMOl and SEC24a/ERMO2 Are Required for Maintenance of Endoplasmic Reticulum Morphology in Arabidopsis thaliana2009

    • Author(s)
      Nakano, R.T., Matsushima, R., Ueda, H., Tamura, K., Shimada, T., Li, L., Hayashi, Y., Kondo, M., Nishimura, M.
    • Journal Title

      Plant Cell 21

      Pages: 36723685-36723685

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging.2008

    • Author(s)
      Matsushima, R., Hamamura, Y., Higashiyama, T., Arimura, S. -i., Sodmergen, Tsutsumi, N., Sakamoto, W.
    • Journal Title

      Plant and Cell Physiology Vol.49

      Pages: 1074-1083

    • NAID

      10027332435

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The model plant Medicago truncatula exhibits biparental plastid inheritance.2008

    • Author(s)
      Matsushima, R., Hu, Y., Toyoda, K., Sodmergen, Sakamoto, W.
    • Journal Title

      Plant and Cell Physiology Vol.49

      Pages: 81-91

    • NAID

      10027495231

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The model plant Medicago truncatula exhibits biparental plastid inheritance2008

    • Author(s)
      Matsushima, R, Hu, Y., Toyoda. K, Sodmergen. Sakamoto, W.
    • Journal Title

      Plant and Cell Physiology 49

      Pages: 81-91

    • NAID

      10027495231

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging2008

    • Author(s)
      Matsushima, R., Hamamura, Y., Higashiyama, T., Arimura. Sodmergen. Tsutsumi. N., Sakamoto, W.
    • Journal Title

      Plant and Cell Physiology 49

      Pages: 1074-1083

    • NAID

      10027332435

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] デンプン粒の形状多様性を支配する分子機構の解明にむけて.2009

    • Author(s)
      松島良・前川雅彦・藤田直子・山下純・坂本亘
    • Organizer
      植物細胞生物学若手の会
    • Place of Presentation
      奈良
    • Related Report
      2009 Final Research Report
  • [Presentation] 植物の雄性配偶体形成におけるオルガネラDNAのダイナミクス.2009

    • Author(s)
      坂本亘・松島良
    • Organizer
      第81回日本遺伝学会
    • Place of Presentation
      松本
    • Related Report
      2009 Final Research Report
  • [Presentation] 小胞体の形態と細胞内分布に異常を示すendoplasmic reticulum morphology (ermo)変異体の解析2009

    • Author(s)
      中野亮平・松島良・上田晴子・林八寿子・田村謙太郎・嶋田知生・西村いくこ
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report
  • [Presentation] 花粉においてオルガネラDNAの分解を担う遺伝子の単離と解析.2009

    • Author(s)
      松島良・Lay Yin Tang・蘇都莫日根・David Twell・坂本亘
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report
  • [Presentation] endoplasmic reticulum morphology (ermo) mutants of Arabidopsis thaliana develop a number of novel ER-derived structures in the cells.2008

    • Author(s)
      Nakano, T.R., Matsushima, R., Ueda, H., Hayashi, Y., Tamura, K., Shimada, T., Hara-Nishimura, I.
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同学会
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report
  • [Presentation] 花粉における細胞質ゲノムの分解機構に関する研究.2008

    • Author(s)
      松島良・坂本亘
    • Organizer
      国立遺伝学研究所研究集会「高等植物の生殖過程を制御する因子と生殖隔離」
    • Place of Presentation
      三島
    • Related Report
      2009 Final Research Report
  • [Presentation] Mitochondrial dynamics in plant male gametophyte studied by fluorescent live imaging.2008

    • Author(s)
      Matsushima, R., Hamamura, Y., Higashiyama, T., Sakamoto, W.
    • Organizer
      Frontiers of Sexual Plant Reproduction III., USA
    • Place of Presentation
      Arizona
    • Related Report
      2009 Final Research Report
  • [Presentation] Pollen organellar DNA retention in ribonucleotide reductase mutants of Arabidopsis.2008

    • Author(s)
      Tang, L.Y., Matsushima, R., Sakamoto, W.
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Presentation] Mitochondrial dynamics in plant male gametophyte studied by fluorescent live imaging2008

    • Author(s)
      Matsushima, R., Hamamura, Y., Higashiyama, T. and Sakamoto, W.
    • Organizer
      Frontiers of Sexual Plant Reproduction III.
    • Place of Presentation
      Tucson, Arizona, USA
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.rib.okayama-u.ac.jp/saka/index-j.html

    • Related Report
      2009 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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