Gene expression regulated by small RNA and DNA methylation in higher plants

• Project/Area Number: 20770038
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: HOSHINO Atsushi National Institute for Basic Biology, 分子遺伝学研究部門, 助教 (80312205)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 高等植物 / 低分子RNA / DNAメチル化 / RNAi / 花 / 植物 / ゲノム / 遺伝子

Research Abstract

Small RNA and DNA methylation are involved in fundamental systems to control gene expression. To reveal their dynamics and roles on gene regulation in higher plants, we studied the flower pigmentation patterns which are thought to be controlled by small RNA and DNA methylation in the Japanese morning glory. The results suggested that small RNA amplification is a key mechanism to produce the pigmentation patterns.

Research Products

• [Journal Article] Constitutive expression of the GIGANTEA ortholog affects circadian rhythms and suppresses one-shot induction of flowering in Pharbitis nil, a typical short-day plant. (2011)
  • Author(s): Y.Higuchi, K.Sage-Ono, R.Sasaki, N.Ohtsuki, A.Hoshino, S.Iida, H.Kamada, M.Ono
  • Journal Title: Plant Cell Physiol. 52 Pages: 638-650
  • NAID: 10029710157
  • Peer Reviewed
• [Journal Article] Constitutive expression of the GIGANTEA ortholog affects circadian rhythms and suppresses one-shot induction of flowering in Pharbitis nil, a typical short-day plant. (2011)
  • Author(s): Y.Higuchi, K.Sage-Ono, R.Sasaki, N.Ohtsuki, A.Hoshino, S.Iida, H.Kamada, M.Ono
  • Journal Title: Plant Cell Physiol. Volume: 52(印刷中)
  • NAID: 10029710157
  • Peer Reviewed
• [Journal Article] Identification of r mutations conferring white flowers in the Japanese morning glory, Ipomoea nil. (2009)
  • Author(s): A.Hoshino, K.I.Park, S.Iida
  • Journal Title: J.Plant Research 122 Pages: 215-222
  • NAID: 10025179695
  • Peer Reviewed
• [Journal Article] Characterization of autonomous Dart1 transposons belonging to the hAT superfamily in rice. (2009)
  • Author(s): Z.Shimatani, K.Takagi, C.H.Eun, M.Maekawa, H.Takahara, A.Hoshino, Q.Qian, R.Terada, Y.Johzuka-Hisatomi, S.Iida, K.Tsugane
  • Journal Title: Mol.Gen.Genomics 281 Pages: 329-344
  • Peer Reviewed
• [Journal Article] Identification of r mutations conferring white flowers in the Japanese morning glory (Ipomoea nil) (2009)
  • Author(s): A. Hoshino
  • Journal Title: Journal of Plant Research 122 Pages: 215-222
  • NAID: 10025179695
  • Peer Reviewed
• [Journal Article] Characterization of autonomous Dart1 transposons belonging to the hAT superfamily in rice (2009)
  • Author(s): Z. Shimatani
  • Journal Title: Molecular Genetics and Genomics 281 Pages: 329-344
  • Peer Reviewed
• [Journal Article] The gravity-regulated growth of axillary buds is mediated by a mechanism different from decapitation-induced release. (2008)
  • Author(s): D.Kitazawa, Y.Miyazawa, N.Fujii, A.Hoshino, S.Iida, E.Nitasaka, H.Takahashi
  • Journal Title: Plant Cell Physiol. 49 Pages: 891-900
  • NAID: 10027331719
  • Peer Reviewed
• [Journal Article] The anthocyanin pigmentation controlled by the speckled and c-1 mutations of the Japanese morning glory.
  • Author(s): N.Saito, F.Tatsuzawa, A.Hoshino, Y.Abe, M.Ichimura, M.Yokoi, K.Toki, Y.Morita, S.Iida, T.Honda
  • Journal Title: J.Japan.Soc.Hort.Sci. in press
  • NAID: 130004951646
  • Peer Reviewed
• [Presentation] 伝統園芸植物・アサガオの多彩な花色の発現機構 (2011)
  • Author(s): 星野敦
  • Organizer: 5回アントシアニン研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-03-03
• [Presentation] ソライロアサガオの模様に係わるエピジェネティクス (2011)
  • Author(s): 星野敦、朴慶一、崔丁斗、飯田滋
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] ダリア花弁の純白化に関わる2種類のCHSの同時的転写後分解 (2011)
  • Author(s): 細川宗孝、大野翔、星野敦、立澤文見、土井元章
  • Organizer: 園芸学会平成23年度春季大会
  • Place of Presentation: 宇都宮
• [Presentation] ソライロアサガオの模様に係わるエピジェネティクス (2011)
  • Author(s): 星野敦、朴慶一、崔丁斗、飯田滋
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 東北大学(岩手県)
• [Presentation] Epigenetic Regulation of Flower Variegation in the Morning Glory. (2010)
  • Author(s): Atsushi Hoshino
  • Organizer: The 2nd NIBB-MPIPZ Joint Symposium, Plant Science Communications 2010
  • Place of Presentation: Okazaki, Japan
• [Presentation] アサガオの動かないトランスポゾンと花の模様 (2010)
  • Author(s): 星野敦
  • Organizer: 第6回アサガオ研究集会
  • Place of Presentation: 岡崎
• [Presentation] ナショナルバイオリソースプロジェクト・アサガオ (2010)
  • Author(s): 仁田坂英二、星野敦
  • Organizer: 第6回アサガオ研究集会
  • Place of Presentation: 岡崎
• [Presentation] アサガオにおけるアクアポリンの発現と花弁特異的アクアポリン発現抑制形質転換体の作出 (2010)
  • Author(s): 白武勝裕、中川喜夫、牧野治子、水野祐輔、森本玲奈、星野敦、田中幸子、森田裕将、飯田滋
  • Organizer: 第6回アサガオ研究集会
  • Place of Presentation: 岡崎
• [Presentation] mRNAの網羅的塩基配列解読によるアサガオSNPマーカーの作出 (2010)
  • Author(s): リートン、福岡浩之、星野敦、飯田滋、仁田坂英二、久保山勉
  • Organizer: 日本育種学会2010年秋季大会
  • Place of Presentation: 秋田
• [Presentation] mRNAの網羅的塩基配列解読によるアサガオSNPマーカーの作出 (2010)
  • Author(s): リートン、福岡浩之、星野敦、飯田滋、仁田坂英二、久保山勉
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学(秋田県)
• [Presentation] Epigenetic regulation of flower variegation in the morning glory (2010)
  • Author(s): Atsushi Hoshino
  • Organizer: The 2nd NIBB-MPIPZ Joint Symposium, Plant Science Communications 2010
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県)
• [Presentation] アサガオにおけるWUSCHEL、SHOOTMERISTEMLESS、FRUITFULオーソログの単離と解析 (2009)
  • Author(s): 小林奈通子、田野井慶太郎、星野敦、飯田滋、中西友子
  • Organizer: 日本植物生理学会・2009年度年会
  • Place of Presentation: 名古屋
• [Presentation] トランスポゾンが生み出した多様なアサガオの変異体 (2009)
  • Author(s): 仁田坂英二、星野敦、飯田滋
  • Organizer: 日本植物生理学会・2009年度年会
  • Place of Presentation: 名古屋
• [Presentation] 花の模様のエピジェネティクス (2008)
  • Author(s): 星野敦、朴慶一、崔丁斗、飯田滋
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋
• [Presentation] RNAサイレンシングによるアサガオ花弁の模様形成 (2008)
  • Author(s): 森田裕将、星野敦、飯田滋
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋
• [Presentation] Epigenetic regulation of flower pigmentation in the morning glory. (2008)
  • Author(s): Atsushi Hoshino, Jeong-Doo Choi, Kyeung-Il Park, Shigeru Iida
  • Organizer: The 55th NIBB Conference and Arabidopsis Workshop 2008, Frontiers of Plant Science in the 21st Century
  • Place of Presentation: Okazaki, Japan
• [Presentation] 朝顔は江戸からのタイムカプセル:遺伝資源を活かして生き物を知る (2008)
  • Author(s): 星野敦
  • Organizer: 未来エネルギー研究会・第8回マースクール
  • Place of Presentation: 亀岡
• [Presentation] Epigenetic regulation of flower pigmentation in the morning glory (2008)
  • Author(s): A. Hoshino
  • Organizer: The 55th NIBB Conference and Arabidopsis Workshop 2008
  • Place of Presentation: 岡崎
• [Book] アサガオとイネのDNAメチル化と遺伝子発現。細胞工学別冊、植物細胞工学シリーズ24 (2008)
  • Author(s): 定塚(久富)恵世、星野敦、森田裕将、山内卓樹、朴慶一、寺田理恵、森藤暁、飯田滋
  • Publisher: 植物のエピジェネティクス
• [Remarks] ホームページ等
  • URL: http://www.nibb.ac.jp/hoshino