Live-image Analysis on whole developmental process of Oikopleura

• Project/Area Number: 20770046
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphology/Structure
• Research Institution: Osaka University
• Principal Investigator: NISHINO Atsuo Osaka University, 理学研究科, 助教 (50343116)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 蛍光イメージング / ワカレオタマボヤ / 発生 / 尾索動物

Research Abstract

Eggs of Oikopleura (Appendicularia, Tunicata) build a functional juvenile body in the shortest time period and with the least number of cells among chordate organisms. We aimed to comprehend all the cell divisions, cell positions, and the differentiation status from cleavage stage to juvenile stage in Oikopleura development using live-imaging and genetic techniques. Our effort in this study established reliable markers that can track nuclei and plasma membrane, and discovered a subcellular apparatus in cleavage staged embryos that is involved in pulling the nucleus before the cytokinesis to make an unequal cleavage.

Research Products

• [Journal Article] A glycine receptor is involved in the orga nization of swimming movements in an inve rtebrate chordate (2010)
  • Author(s): Nishino A, Okamura Y, Piscopo S, Brown E R
  • Journal Title: BMC Neuroscience 11 Pages: 1-12
  • Peer Reviewed
• [Journal Article] A glycine receptor is involved in the organization of swimming movements in an invertebrate chordate. (2010)
  • Author(s): Nishino A, Okamura Y, Piscopo S, Brown ER
  • Journal Title: BMC Neuroscience 11
  • Peer Reviewed
• [Journal Article] Cooper EC Ion channel clustering at the axon initial segment and node of Ranvier evolved sequentially in early chordates (2008)
  • Author(s): Hill SA, Nishino A, Nakajo K, Zhang G, Fineman JR, Selzer ME, Okamura Y
  • Journal Title: PLoS Genetics 4 Pages: 1-15
  • Peer Reviewed
• [Journal Article] Ion channel clustering at the axon initial segment and node of Ranvier evolved sequentially in early chordates. (2008)
  • Author(s): Hill SA, Nishino A, Nakajo K, Zhanz G. Fineman JR., et.al.
  • Journal Title: PLoS Genetics 4
  • Peer Reviewed
• [Presentation] オタマボヤにおけるCentrosome Attracting Body (2009)
  • Author(s): 幸西翔平、西野敦雄、西田宏記
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡県コンベンションアーツセンターグランシップ
  • Year and Date: 2009-09-17
• [Presentation] オタマボヤにおけるCentrosome Attracting Body (2009)
  • Author(s): 幸西翔平、西野敦雄、西田宏記
  • Organizer: 日本動学会 第80回大会
  • Place of Presentation: 静岡県コンベンションアーツセンター グランシップ
  • Year and Date: 2009-09-17
• [Presentation] ホヤ幼生遊泳運動の形成機構とACh受容体・Gly受容体の役割 (2008)
  • Author(s): 西野敦雄
  • Organizer: 日本動物学会第79回大会関連集会(ホヤの生物学談話会)
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-05
• [Presentation] Morphological evolution and physiological evolution: a molecular basis for ascidian's "simple" tadpole to fluently swim. (2008)
  • Author(s): Nishino A, Baba SA, Okamura Y
  • Organizer: The 41st annual meeting for the Japanese society of developmental biologists
  • Place of Presentation: Tokushima Arts Foundation for Culture, Tokushima, Japan
  • Year and Date: 2008-05-28
• [Presentation] Morphological evolution and physiological evolution : a molecular basis for ascidian's "simple" tadpole to fluently swim. (2008)
  • Author(s): Nishino A, Baba SA, Okamura Y
  • Organizer: The 41st annual meeting for the Japanesesociety of developmental biologists.
  • Place of Presentation: Tokushima Arts Foundation for Culture, To kushima, Japan.
  • Year and Date: 2008-05-28
• [Remarks] 大阪大学研究者総覧内
  • URL: http://www.dma.jim.osaka-u.ac.jp/kg-portal/aspI/RX0011D.asp?UNO=17284&page=