Low-magnification and Wide-field two photon microscope by using femto-second non-diffractive laser beam

• Project/Area Number: 20770051
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphology/Structure
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: FUKANO Takashi The Institute of Physical and Chemical Research, 細胞機能探索技術開発チーム, 研究員 (80373364)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 蛍光顕微鏡 / 2光子蛍光顕微鏡 / フェムト秒レーザー

Research Abstract

We have developed a novel wide-field fluorescence microscope using non-diffractive beam generated with a femto-second laser and an axicon lens. Fluorescence matters in sample can be excited along optical axis by the beam. This microscope allows to observe optically sectioned image with low-magnification objective. We have employed this microscope to image living sample and discussed its biological application.