Structural Basis of tRNA modification at position 37

• Project/Area Number: 20770077
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: ITO Takuhiro The University of Tokyo, 大学院・理学系研究科, 助教 (70401164)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: タンパク質 / 核酸 / tRNA / 修飾塩基 / X線結晶構造解析 / 立体構造

Research Abstract

The crystal structure of the TRM5・tRNA・AdoMet complex was determined. TRM5 is the enzyme that transfers a methyl moiety from a methyl-donor AdoMet to the N1 position of G37 in tRNA. TRM5 has two structural domains, D1 and D2-D3, to recognize a substrate tRNA. We clarified that D2-D3 transfers methyl moiety to G37, only when D1 recognizes the establishment of the L-shaped tRNA structure. Therefore, TRM5 may function as a checkpoint for the quality of the tRNA maturation.

Research Products

• [Journal Article] Tertiary structure checkpoint at anticodon loop modification in tRNA functional maturation (2009)
  • Author(s): Goto-Ito, S., Ito, T., Kuratani, M., Bessho, Y., Yokoyama, S.
  • Journal Title: Nat Struct Mol Biol 16 Pages: 1109-1115
• [Journal Article] Tertiary structure checkpoint at anticodon loop modification in tRNA functional maturation (2009)
  • Author(s): Sakurako Goto-Ito
  • Journal Title: Nat Struct Mol Biol 16 Pages: 1109-1115
  • Peer Reviewed
• [Journal Article] Crystal Structure of Archaeal tRNA (m1G37) methyltransferase aTRM5 (2008)
  • Author(s): Goto-Ito, S., Ito, T., Ishii, R., Muto, Y., Bessho, Y., Yokoyama, S.
  • Journal Title: PROTEINS: Structure, Function, and Bioinformatics 72 Pages: 1274-1289
• [Journal Article] Crystal structure of archaeal tRNA(m(1)G37)methyltransferase aTrm5 (2008)
  • Author(s): Sakurako Goto-Ito
  • Journal Title: Proteins 72 Pages: 1274-1289
  • Peer Reviewed
• [Presentation] Tertiary structure checkpoint in tRNA functional maturation (2010)
  • Author(s): Takuhiro Ito
  • Organizer: 23rd tRNA workshop
  • Place of Presentation: Averio, Portugal
  • Year and Date: 2010-01-29
• [Presentation] Tertiary structure checkpoint in tRNA functional maturation (2010)
  • Author(s): 伊藤(後藤)桜子
  • Organizer: 23^<rd> tRNA workshop
  • Place of Presentation: Averio, Portugal
  • Year and Date: 2010-01-29
• [Presentation] tRNA 37位のグアノシンに対するメチル基転移酵素(Trm5)は, tRNA成熟プロセスにおけるtRNA L字型立体構造のチェックポイントとして働く (2009)
  • Author(s): 伊藤(後藤)桜子
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 朱鷺メッセ新潟コンベンションセンター
  • Year and Date: 2009-07-28
• [Presentation] tRNA37位のグアノシンに対するメチル基転移酵素(Trm5)は, tRNA成熟プロセスにおけるtRNAL字型立体構造のチェックポイントとして働く (2009)
  • Author(s): 伊藤(後藤)桜子
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 朱鷺メッセ 新潟コンベンションセンター
  • Year and Date: 2009-07-28
• [Presentation] tRNA37位のグアノシンに対するメチル基転移酵素(TRM5)によるtRNA認識機構 (2008)
  • Author(s): 伊藤拓宏
  • Organizer: BMB2008
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] tRNA37位のグアノシンに対するメチル基転移酵素(TRM5)によるtRNA認識機構の立体構造学的研究 (2008)
  • Author(s): 伊藤(後藤)桜子
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-07-25
• [Presentation] tRNAアンチコドン隣位(37位)のグアノシンに連続的に働く修飾酵素の立体構造学的研究 (2008)
  • Author(s): 伊藤拓宏
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-07-23