Structural analyses for yeast Asna-1 ATPase (Get3)

• Project/Area Number: 20770078
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: ATSUSHI Yamagata The University of Tokyo, 放射光連携研究機構, 助教 (20463903)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 蛋白質 / 構造生物学 / ATP加水分解酵素 / 膜タンパク質

Research Abstract

Asna1 ATPase can insert the membrane protein containing a single transmembrane domain at the C-terminus, so called as tail anchored protein, dependent on its ATP hydrolysis. In this study, we determined the crystal structures of yeast Asna1 ATPase (Get3) in both ADP bound and nucleotide free forms. In addition, structure based biochemical studies revealed the TA protein biding site in Get3, and its conformational transition upon ATP hydrolysis.

Research Products

• [Journal Article] Structural insight into the membrane insertion of tail-anchored proteins by Get3 (2010)
  • Author(s): Yamagata A, Mimura H, Sato Y, Yamashita M, Yoshikawa A, Fukai S
  • Journal Title: Genes to Cells 15 Pages: 29-41
  • Peer Reviewed
• [Journal Article] Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3 (2010)
  • Author(s): Yamashita M., Kurokawa K., Sato, Y., Yamagata A., Mimura H., Yoshikawa A., Sato K., Nakano A., Fukai S.
  • Journal Title: Nat. Struct. Mol. Biol. 17 Pages: 180-186
  • Peer Reviewed
• [Journal Article] Structural insight into the membrane insertion of tail-anchored proteins by Get3 (2010)
  • Author(s): 山形敦史
  • Journal Title: Genes to Cells 15 Pages: 29-41
  • Peer Reviewed
• [Journal Article] Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3 (2010)
  • Author(s): 山下雅美
  • Journal Title: Nature Structural & Molecular Biology 17 Pages: 180-186
  • Peer Reviewed
• [Journal Article] Structural basis for specific recognition of Lys 63-linked polyubiquitin chains by NZF domains of TAB2 and TAB3 (2009)
  • Author(s): Sato Y., Yoshikawa A., Yamashita M., Yamagata A., Fukai S.
  • Journal Title: Embo J 28 Pages: 3903-3909
  • Peer Reviewed
• [Journal Article] Crystal structure of the NEMO ubiquitin-binding domain in complex with Lys 63-linked di-ubiquitin (2009)
  • Author(s): Yoshikawa A., Sato Y., Yamashita M., Mimura H., Yamagata A., Fukai S.
  • Journal Title: FEBS Lett 583 Pages: 3317-3322
  • Peer Reviewed
• [Journal Article] Structural basis for specific recognition of Lys 63-linked polyubiquitin chains by tandem UIMs of Rap80 (2009)
  • Author(s): Sato Y, Yoshikawa A, Mimura H, Yamashita M, Yamagata A, Fukai S.
  • Journal Title: Embo J 28 Pages: 2461-2468
  • Peer Reviewed
• [Journal Article] Structural basis for specific recognition of Lys 63-linked polyubiquitin chains by NZF domains of TAB2 and TAB3 (2009)
  • Author(s): 佐藤裕介
  • Journal Title: THE EMBO JOURNAL 28 Pages: 3903-3909
  • Peer Reviewed
• [Journal Article] Crystal structure of the NEMO ubiquitin-binding domain in complex with Lys 63-linked di-ubiquitin (2009)
  • Author(s): 吉川梓
  • Journal Title: FEBS Letters 583 Pages: 3317-3322
  • Peer Reviewed
• [Journal Article] Structural basis for specific recognition of Lys 63-linked polyubiquitin chains by tandem UIMs of RAP80 (2009)
  • Author(s): 佐藤裕介
  • Journal Title: THE EMBO JOURNAL 28 Pages: 2461-2468
  • Peer Reviewed
• [Journal Article] Structural basis for specific cleavage of Lys63-linked polyubiquitin chains (2008)
  • Author(s): Sato Y, Yoshikawa A, Yamagata A, Mimira H, Yamashita M, Ookata K, Nureki O, Iwai K, Komada M, Fukai S.
  • Journal Title: Nature 455 Pages: 358-362
  • Peer Reviewed
• [Presentation] Structural insight into the membrane insertion of tail-anchored proteins by the Asna1-family ATPase (2009)
  • Author(s): 山形敦史、三村久敏、佐藤裕介、山下雅美、吉川梓、深井周也
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Structural insight into the membrane insertion of tail -anchored proteins by the Asnal-family ATPase (2009)
  • Author(s): 山形敦史
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Remarks]
  • URL: http://www.iam.u-tokyo.ac.jp/srro/SRROLifeSciDivJp2/Welcome.html
• [Remarks]
  • URL: http://www.iam.u-tokyo.ac.jp/srro/SRROLifeSciDivJp2/Welcome.html