Structure and function of small RNA-protein complexes

• Project/Area Number: 20770079
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: NISHIMASU Hiroshi The University of Tokyo, 大学院・理学系研究科, 特任助教 (00467044)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: タンパク質 / 核酸 / 結晶構造

Research Abstract

We solved the crystal structure of Thermus thermophilus TrmFO, providing a structural basis for the folate/FAD-dependent methylation of tRNA U54. We also solved the crystal structure of Pyrocuccus horikoshii and Methanococcus jannaschii TYW2s, providing a structural basis for the S-adenosylmethionine-dependent transfer of the α-amino-α-carboxypropyl group into tRNA imG-14.

Research Products

• [Journal Article] Crystal structure of autotaxin and insight into GPCR activation by lipid mediators (2011)
  • Author(s): H.Nishimasu, S.Okudaira, K.Hama, E.Mihara, N.Dohmae, A.Inoue, R.Ishitani, J.Takagi, J.Aoki, O.Nureki.
  • Journal Title: Nat Struct Mol Biol 18 Pages: 205-212
  • Peer Reviewed
• [Journal Article] Crystal structure of a PFU-PUL domain pair of Saccharomyces cerevisiae Doa1/Ufd3R. (2010)
  • Author(s): Nishimasu, H.Komori, Y.Higuchi, H.Nishimasu, H.Hiroaki.
  • Journal Title: Kobe J Med Sci 56 Pages: 125-139
  • Peer Reviewed
• [Journal Article] Structural basis of novel interactions between the small-GTPase and GDI-like domains in prokaryotic FeoB iron transporter (2009)
  • Author(s): M.Hattori, Y.Jin, H.Nishimasu, Y.Tanaka, M.Mochizuki, T.Uchiumi, R.Ishitani, K.Ito, O.Nureki.
  • Journal Title: Structure 17 Pages: 1345-1355
  • Peer Reviewed
• [Journal Article] Structural basis of AdoMet-dependent aminocarboxypropyl transfer reaction catalyzed by tRNA-wybutosine synthesizing enzyme, TYW2 (2009)
  • Author(s): M.Umitsu, H.Nishimasu, A.Noma, T.Suzuki, R.Ishitani, O.Nureki.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 15616-15621
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray diffraction analysis of the truncated cytosolic domain of the iron transporter FeoB (2009)
  • Author(s): Y.Jin, M.Hattori, H.Nishimasu, R.Ishitani, O.Nureki.
  • Journal Title: Acta Crystallogr F 65 Pages: 784-787
  • Peer Reviewed
• [Journal Article] Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase (2009)
  • Author(s): H.Nishimasu, R.Ishitani, K.Yamashita, C.Iwashita, A.Hirata, H.Hori, O.Nureki.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 8180-8185
  • Peer Reviewed
• [Journal Article] Structural basis of AdoMet-dependent aminocarboxypropyl transfer reaction catalyzed by tRNA-wybutosine synthesizing enzyme, TYW2 (2009)
  • Author(s): M.Umitsu, H.Nishimasu, A.Noma, T.Suzuki, R.Ishitani, O.Nureki
  • Journal Title: Proc.Natl.Acad.Sci.USA. 106 Pages: 15616-15621
  • Peer Reviewed
• [Journal Article] Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase (2009)
  • Author(s): H.Nishimasu, R.Ishitani, K.Yamashita, C.Iwashita, A.Hirata, H.Hori, O.Nureki
  • Journal Title: Proc.Natl.Acad.Sci.USA. 106 Pages: 8180-8185
  • Peer Reviewed
• [Presentation] Structural basis of AdoMet-dependent aminocarboxypropyl transfer reaction catalyzed by tRNA-wybutosine synthesizing enzyme, TYW2 (2010)
  • Author(s): 西増弘志
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase (2010)
  • Author(s): Hiroshi Nishimasu
  • Organizer: 23rd tRNA workshop
  • Place of Presentation: ポルトガル,アヴェイロ
  • Year and Date: 2010-01-29
• [Presentation] Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase (2010)
  • Author(s): 西増弘志
  • Organizer: 23rd tRNA workshop
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2010-01-29
• [Presentation] 葉酸/FAD依存性tRNA U54メチル化酵素TrmFOの機能と構造 (2009)
  • Author(s): 西増弘志
  • Organizer: 第11回RNAミーティング
  • Place of Presentation: 新潟朱鷺メッセ
  • Year and Date: 2009-07-28
• [Presentation] 葉酸/FAD依存性tRNA U54メチル化酵素TrmFOの機能と構造 (2009)
  • Author(s): 西増弘志、石谷隆一郎、山下光輝, 岩下知香子, 平田章、堀弘幸、濡木理
  • Organizer: 第11回RNAミーティング
  • Place of Presentation: 新潟・朱鷺メッセ
  • Year and Date: 2009-07-28
• [Presentation] 1.0-A resolution structure of a new RNA modification enzyme (2009)
  • Author(s): Hiroshi Nishimasu
  • Organizer: Gordon Research Conferences (RNA editing)
  • Place of Presentation: アメリカ,テキサス
  • Year and Date: 2009-01-12
• [Book] 実験医学増刊 拡大・進展を続けるRNA研究の最先端 (2010)
  • Author(s): 西増弘志, 濡木理
  • Total Pages: 207
  • Publisher: 羊土社
• [Book] 実験医学増刊拡大・進展を続けるRNA研究の最先端 (2010)
  • Author(s): 西増弘志
  • Total Pages: 207
  • Publisher: 羊土社