Structure analysis of the flagellar hook-basal body with the C ring by electron cryomicroscopy

• Project/Area Number: 20770083
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: MIYATA Tomoko Osaka University, 大学院・生命機能研究科, 特任研究員 (30423156)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 分子モーター / 電子顕微鏡 / 単粒子解析

Research Abstract

Bacterial flagella has a reversible rotary motor that can rotate in both counterclockwise(CCW) and clockwise(CW) direction. The switching of the motor rotation direction is controlled by the complex located at the bottom of the basal body called the C ring, constructed from switch proteins. To understand the switching mechanisms of motor rotation in detail, we analyzed the C ring structures of the wild type and the CW-bias mutant by electron cryomicroscopy. Conformational change of the C ring by rotation direction switching was observed by the structure comparison between the wild type and the CW -bias mutant.

Research Products

• [Journal Article] Structure of the 100S ribosome in the hibernation stage revealed by electron cryomicroscopy (2010)
  • Author(s): Kato, T., Yoshida, D., Miyata, T., Maki, Y.,Wada, A., Namba, K.
  • Journal Title: Structure (in press)
  • Peer Reviewed
• [Journal Article] Structure of the 100S ribosome in the hibernation stage revealed by electron cryomicroscopy. (2010)
  • Author(s): Kato, T., et al
  • Journal Title: Structure (in press)
  • Peer Reviewed
• [Journal Article] ATP-induced FliI hexamerization facilitates bacterial flagellar protein export (2009)
  • Author(s): Kazetani, K., Minamino, T., Miyata, T., Kato,T., Namba, K.
  • Journal Title: Biochemical and BiophysicalResearch Communications 388 Pages: 323-327
  • Peer Reviewed
• [Journal Article] ナノスケールの回転モーター (2009)
  • Author(s): 宮田知子
  • Journal Title: 未来材料 9 Pages: 6-8
• [Journal Article] Construction or two color semiconductor quantum dots wire by utilizing the complementarity of DNA (2008)
  • Author(s): Tanaka, S., Miyata, T., Kato, T., Namba, K.,Yanagida, T., Sako, Y., Kawata, S., Inoue,Y.
  • Journal Title: AIP Cpnf. Proc. 1062 Pages: 116-122
  • Peer Reviewed
• [Journal Article] Construction or two color semiconductor quantum dots wire by utiliiing the complementarity of DNA (2008)
  • Author(s): Tanaka, S.
  • Journal Title: AIP Cpnf. Proc 1062 Pages: 116-122
  • Peer Reviewed
• [Presentation] Structural analysis of the FliH/FliI complex of the flagellar type III protein export apparatus with phase contrast electron cryotomography (2010)
  • Author(s): Kawamoto, A., Minamino, T., Miyata, T., Kato, T., Shigematsu, H., Hosogi, N., Nagayama, K., Namba, K.
  • Organizer: Gordon Research Conference on Biomolecular Interactions & Methods
  • Place of Presentation: Hotel Galvez, USA
• [Presentation] Structural analysis of the F1iH/FliI complex of the flagellar type III prote in export apparatus with phase contrast electron cryotomography. (2010)
  • Author(s): Kawamoto, A., etal
  • Organizer: Gordon Research Conference on Biomolecular Interactions & Methods
  • Place of Presentation: Hotel Galvez, USA Galvesfon
• [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析 (2009)
  • Author(s): 宮田知子, 加藤貴之, 藤井高志, 松波秀行, 難波啓一
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2009-12-02
• [Presentation] 電子線位相差トモグラフィーによるべん毛タイプIIIタンパク質輸送装置FliH/FliI複合体の構造解析 (2009)
  • Author(s): 川本晃大, 南野徹, 宮田知子, 加藤貴之, 重松秀樹, 細木直樹, 永山國昭, 難波啓一
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2009-12-02
• [Presentation] 低温電子顕微鏡法による生体超分子複合体の立体構造解析に対する電子線照射損傷の定量的評価 (2009)
  • Author(s): 葦原雅道, 加藤貴之, 藤井高志, 松波秀行, 宮田知子, 難波啓一
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2009-12-02
• [Presentation] 低温電子顕微鏡法による生体超分子複合体の立体構造解析に対する電子線照射損傷の定量的評価 (2009)
  • Author(s): 葦原雅道,etal
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター
  • Year and Date: 2009-12-02
• [Presentation] 電子線位相差トモグラフィーによるべん毛タイプIIIタンパク質輸送装置FliH/FIiI複合体の構造解析. (2009)
  • Author(s): 川本晃大,etal
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター
  • Year and Date: 2009-12-02
• [Presentation] べん毛タイプIII蛋白質輸送装置FliH/FliI複合体の構造解析に向けた試料調製 (2009)
  • Author(s): Kawamoto, A., Minamino, T., Miyata, T., Kato, T., Namba, K.
  • Organizer: Steps towards structural analysis of the FliH/FliI complex of the flagellar type III protein export apparatus. 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま/徳島文理大学
  • Year and Date: 2009-11-01
• [Presentation] べん毛タイプIII蛋白質輸送装置FliH/FliI複合体の構造解析に向けた試料調製.Steps towards structural analysis of the FliH/Flil complex of the flagellar type III protein export apparatus. (2009)
  • Author(s): Kawamoto, A., eta
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま/徳島文理大学
  • Year and Date: 2009-11-01
• [Presentation] 低温電子顕微鏡法における生体超分子複合体に対する電子線照射損傷の定量的評価 (2009)
  • Author(s): Ashihara, M., Kato, T., Fujii, T., Matsunami, H., Miyata, T., Iwasaki, K., Namba, K.
  • Organizer: Electron dose effect on 3D image reconstruction of a biological macromolecular structure by electron cryomicroscopy. 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま/徳島文理大学
  • Year and Date: 2009-10-31
• [Presentation] 細菌べん毛モーター・スイッチ複合体の極低温電子顕微鏡による構造解析 (2009)
  • Author(s): Miyata, T., Kato, T., Fujii, T., Matsunami, H., Namba, K.
  • Organizer: Structural analysis of the flagellar hook-basal boday with the Cring by electron cryomicroscopy. 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま/徳島文理大学
  • Year and Date: 2009-10-31
• [Presentation] FliIとFliJの構造より明らかとなったべん毛輸送装置とF1-ATPaseにおける共通の構造 (2009)
  • Author(s): Ibuki, T., Minamino, T., Miyata, T., Kato, T., Namba, K., Imada, K.
  • Organizer: Common architecture between the flagellar export apparatus and F1- ATPase revealed by the structure of FliI and FliJ. 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま/徳島文理大学
  • Year and Date: 2009-10-31
• [Presentation] 低温電子顕微鏡法における生体超分子複合体に対する電子線照射損傷の定量的評価.Electron dose effect on 3D image reconstruction of a biological macromolecular structure by electron cryomicrospy. (2009)
  • Author(s): Ashihara, M., etal
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま/徳島文理大学
  • Year and Date: 2009-10-31
• [Presentation] FliIとFliJの構造より明らかとなったべん毛輸送装置とF1-ArPaseにおける共通の構造.Common architecture between the flagellar export apparatus and F1-ATPase revealed by the structure of Flil and FliJ. (2009)
  • Author(s): Ibuki, T., etal
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま/徳島文理大学
  • Year and Date: 2009-10-31
• [Presentation] 細菌べん毛モーター・スイッチ複合体の極低温電子顕微鏡による構造解析.Structural analysis of the flagellar hook-basal boday with the Cring by electron cryomicroscopy. (2009)
  • Author(s): Miyata, T., etal
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま/徳島文理大学
  • Year and Date: 2009-10-31
• [Presentation] 細菌べん毛フック繊維連結部の極低温電子顕微鏡による構造解析 (2009)
  • Author(s): Makino, F., Kato, T., Miyata, T., Namba, K.
  • Organizer: Structural analysis of the flagellar hook-filament junction by electron cryomicroscopy. 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま/徳島文理大学
  • Year and Date: 2009-10-30
• [Presentation] 細菌べん毛フック繊維連結部の極低温電子顕微鏡による構造解析.Structural analysis of the flagellar hook-filament junction by electron cryomicrospy. (2009)
  • Author(s): Makino, F., etal
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: アスティとくしま/徳島文理大学
  • Year and Date: 2009-10-30
• [Presentation] Electron dose effect on 3D image reconstruction of a biological macromolecular structure by electron cryomicroscopy (2009)
  • Author(s): Ashihara, M., Kato T., Fujii, T., Matsunami H., Miyata T., Namba K.
  • Organizer: EMBO Course on Image Processing for CryoEM
  • Place of Presentation: Birkbeck College, London, U.K.
  • Year and Date: 2009-09-10
• [Presentation] Electron dose effect on 3D image reconstruction of a biological macromolecular structure by electron cryomicrospy. (2009)
  • Author(s): Ashihara, M., etal
  • Organizer: EMBO Course on Image Processing for CryoEM
  • Place of Presentation: Birkbeck College, U.K
  • Year and Date: 2009-09-10
• [Presentation] Common architecture between the flagellar export apparatus and F1- ATPase revealed by the structure of FliI and FliJ (2009)
  • Author(s): Ibuki, T., Minamino, T., Miyata, T., Kato, T., Namba, K., Imada, K.
  • Organizer: International Symposium "Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes"
  • Place of Presentation: Shirankaikan, Japan
  • Year and Date: 2009-09-08
• [Presentation] Common architecture between the flagellar export apparatus and F_-ATPase revealed by the structure of Flil and FliJ. (2009)
  • Author(s): Ibuki, T., etal
  • Organizer: International Symposium "Innovative Nanoscience of Supermolecular Motor Proteins Working in Bio membranes"
  • Place of Presentation: Shlrankalkan 京都大学
  • Year and Date: 2009-09-08
• [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析. (2009)
  • Author(s): 宮田知子
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回ワークショップ
  • Place of Presentation: 湘南国際村センター国際会議場
  • Year and Date: 2009-07-28
• [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析 (2009)
  • Author(s): 宮田知子
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回ワークショップ
  • Place of Presentation: 湘南国際村センター国際会議場
  • Year and Date: 2009-07-27
• [Presentation] PAX6-SOX2-DNA複合体の塩基配列特異的な構造変化と遺伝子スイッチ機能 (2008)
  • Author(s): 近藤寿人, 蒲池雄介, 宮田知子, 加藤貴之, 今田勝巳
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回公開シンポジウム
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2008-12-19
• [Presentation] 細菌べん毛基部体の構造解析 (2008)
  • Author(s): 加藤貴之, 宮田知子, 藤井高志, 松波秀行, 難波啓一
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回公開シンポジウム
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2008-12-18
• [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析 (2008)
  • Author(s): 宮田知子, 加藤貴之, 藤井高志, 松波秀行, 難波啓一
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回公開シンポジウム
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2008-12-18
• [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析 (2008)
  • Author(s): Miyata, T., Kato, T., Fujii, T., Matsunami, H., Namba, K.
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-05
• [Presentation] 極低温電子顕微鏡によるべん毛タイプIII蛋白質輸送装置の構造および機能解析 (2008)
  • Author(s): Kawamoto, A., Minamino, T., Miyata, T., Kato, T., Namba, K.
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-03
• [Presentation] Optimal conditions for electron cryomicroscopy with liquid helium-cooled specimen stage. Gordon Research Conference on Three Dimensional Electron Microscopy (2008)
  • Author(s): Ashihara, M., Matsunami, H., Miyata, T., Namba, K.
  • Organizer: II Ciocco Hotel and Resort
  • Place of Presentation: Lucca (Barga), Italy
• [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析 (2008)
  • Author(s): 宮田知子
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第4回ワークショップ
  • Place of Presentation: 淡路夢舞台国際会議場
• [Presentation] Optimal conditions for electron cryomicroscopy with liquid helium-cooled specimen stage (2008)
  • Author(s): Ashihara, M.
  • Organizer: Gordon Research Conference on Three Dimensional Electron Microscopy
  • Place of Presentation: II Ciocco Hotel and Resort, Lucca (Barga), Italy
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/jp/labo/02
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labo/09a.html
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labo/09a.html