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Structure analysis of the flagellar hook-basal body with the C ring by electron cryomicroscopy

Research Project

Project/Area Number 20770083
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

MIYATA Tomoko  Osaka University, 大学院・生命機能研究科, 特任研究員 (30423156)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords分子モーター / 電子顕微鏡 / 単粒子解析
Research Abstract

Bacterial flagella has a reversible rotary motor that can rotate in both counterclockwise(CCW) and clockwise(CW) direction. The switching of the motor rotation direction is controlled by the complex located at the bottom of the basal body called the C ring, constructed from switch proteins. To understand the switching mechanisms of motor rotation in detail, we analyzed the C ring structures of the wild type and the CW-bias mutant by electron cryomicroscopy. Conformational change of the C ring by rotation direction switching was observed by the structure comparison between the wild type and the CW -bias mutant.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (40 results)

All 2010 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (31 results) Remarks (3 results)

  • [Journal Article] Structure of the 100S ribosome in the hibernation stage revealed by electron cryomicroscopy2010

    • Author(s)
      Kato, T., Yoshida, D., Miyata, T., Maki, Y.,Wada, A., Namba, K.
    • Journal Title

      Structure (in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure of the 100S ribosome in the hibernation stage revealed by electron cryomicroscopy.2010

    • Author(s)
      Kato, T., et al
    • Journal Title

      Structure (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ATP-induced FliI hexamerization facilitates bacterial flagellar protein export2009

    • Author(s)
      Kazetani, K., Minamino, T., Miyata, T., Kato,T., Namba, K.
    • Journal Title

      Biochemical and BiophysicalResearch Communications 388

      Pages: 323-327

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] ナノスケールの回転モーター2009

    • Author(s)
      宮田知子
    • Journal Title

      未来材料 9

      Pages: 6-8

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Construction or two color semiconductor quantum dots wire by utilizing the complementarity of DNA2008

    • Author(s)
      Tanaka, S., Miyata, T., Kato, T., Namba, K.,Yanagida, T., Sako, Y., Kawata, S., Inoue,Y.
    • Journal Title

      AIP Cpnf. Proc. 1062

      Pages: 116-122

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Construction or two color semiconductor quantum dots wire by utiliiing the complementarity of DNA2008

    • Author(s)
      Tanaka, S.
    • Journal Title

      AIP Cpnf. Proc 1062

      Pages: 116-122

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Structural analysis of the FliH/FliI complex of the flagellar type III protein export apparatus with phase contrast electron cryotomography2010

    • Author(s)
      Kawamoto, A., Minamino, T., Miyata, T., Kato, T., Shigematsu, H., Hosogi, N., Nagayama, K., Namba, K.
    • Organizer
      Gordon Research Conference on Biomolecular Interactions & Methods
    • Place of Presentation
      Hotel Galvez, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] Structural analysis of the F1iH/FliI complex of the flagellar type III prote in export apparatus with phase contrast electron cryotomography.2010

    • Author(s)
      Kawamoto, A., etal
    • Organizer
      Gordon Research Conference on Biomolecular Interactions & Methods
    • Place of Presentation
      Hotel Galvez, USA Galvesfon
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析2009

    • Author(s)
      宮田知子, 加藤貴之, 藤井高志, 松波秀行, 難波啓一
    • Organizer
      特定領域研究「生体超分子の構造形成と機能制御の原子機構」第6回公開シンポジウム
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Year and Date
      2009-12-02
    • Related Report
      2009 Final Research Report
  • [Presentation] 電子線位相差トモグラフィーによるべん毛タイプIIIタンパク質輸送装置FliH/FliI複合体の構造解析2009

    • Author(s)
      川本晃大, 南野徹, 宮田知子, 加藤貴之, 重松秀樹, 細木直樹, 永山國昭, 難波啓一
    • Organizer
      特定領域研究「生体超分子の構造形成と機能制御の原子機構」第6回公開シンポジウム
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Year and Date
      2009-12-02
    • Related Report
      2009 Final Research Report
  • [Presentation] 低温電子顕微鏡法による生体超分子複合体の立体構造解析に対する電子線照射損傷の定量的評価2009

    • Author(s)
      葦原雅道, 加藤貴之, 藤井高志, 松波秀行, 宮田知子, 難波啓一
    • Organizer
      特定領域研究「生体超分子の構造形成と機能制御の原子機構」第6回公開シンポジウム
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Year and Date
      2009-12-02
    • Related Report
      2009 Final Research Report
  • [Presentation] 低温電子顕微鏡法による生体超分子複合体の立体構造解析に対する電子線照射損傷の定量的評価2009

    • Author(s)
      葦原雅道,etal
    • Organizer
      特定領域研究「生体超分子の構造形成と機能制御の原子機構」第6回公開シンポジウム
    • Place of Presentation
      千里ライフサイエンスセンター
    • Year and Date
      2009-12-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] 電子線位相差トモグラフィーによるべん毛タイプIIIタンパク質輸送装置FliH/FIiI複合体の構造解析.2009

    • Author(s)
      川本晃大,etal
    • Organizer
      特定領域研究「生体超分子の構造形成と機能制御の原子機構」第6回公開シンポジウム
    • Place of Presentation
      千里ライフサイエンスセンター
    • Year and Date
      2009-12-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] べん毛タイプIII蛋白質輸送装置FliH/FliI複合体の構造解析に向けた試料調製2009

    • Author(s)
      Kawamoto, A., Minamino, T., Miyata, T., Kato, T., Namba, K.
    • Organizer
      Steps towards structural analysis of the FliH/FliI complex of the flagellar type III protein export apparatus. 日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま/徳島文理大学
    • Year and Date
      2009-11-01
    • Related Report
      2009 Final Research Report
  • [Presentation] べん毛タイプIII蛋白質輸送装置FliH/FliI複合体の構造解析に向けた試料調製.Steps towards structural analysis of the FliH/Flil complex of the flagellar type III protein export apparatus.2009

    • Author(s)
      Kawamoto, A., eta
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま/徳島文理大学
    • Year and Date
      2009-11-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] 低温電子顕微鏡法における生体超分子複合体に対する電子線照射損傷の定量的評価2009

    • Author(s)
      Ashihara, M., Kato, T., Fujii, T., Matsunami, H., Miyata, T., Iwasaki, K., Namba, K.
    • Organizer
      Electron dose effect on 3D image reconstruction of a biological macromolecular structure by electron cryomicroscopy. 日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま/徳島文理大学
    • Year and Date
      2009-10-31
    • Related Report
      2009 Final Research Report
  • [Presentation] 細菌べん毛モーター・スイッチ複合体の極低温電子顕微鏡による構造解析2009

    • Author(s)
      Miyata, T., Kato, T., Fujii, T., Matsunami, H., Namba, K.
    • Organizer
      Structural analysis of the flagellar hook-basal boday with the Cring by electron cryomicroscopy. 日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま/徳島文理大学
    • Year and Date
      2009-10-31
    • Related Report
      2009 Final Research Report
  • [Presentation] FliIとFliJの構造より明らかとなったべん毛輸送装置とF1-ATPaseにおける共通の構造2009

    • Author(s)
      Ibuki, T., Minamino, T., Miyata, T., Kato, T., Namba, K., Imada, K.
    • Organizer
      Common architecture between the flagellar export apparatus and F1- ATPase revealed by the structure of FliI and FliJ. 日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま/徳島文理大学
    • Year and Date
      2009-10-31
    • Related Report
      2009 Final Research Report
  • [Presentation] 低温電子顕微鏡法における生体超分子複合体に対する電子線照射損傷の定量的評価.Electron dose effect on 3D image reconstruction of a biological macromolecular structure by electron cryomicrospy.2009

    • Author(s)
      Ashihara, M., etal
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま/徳島文理大学
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] FliIとFliJの構造より明らかとなったべん毛輸送装置とF1-ArPaseにおける共通の構造.Common architecture between the flagellar export apparatus and F1-ATPase revealed by the structure of Flil and FliJ.2009

    • Author(s)
      Ibuki, T., etal
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま/徳島文理大学
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細菌べん毛モーター・スイッチ複合体の極低温電子顕微鏡による構造解析.Structural analysis of the flagellar hook-basal boday with the Cring by electron cryomicroscopy.2009

    • Author(s)
      Miyata, T., etal
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま/徳島文理大学
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細菌べん毛フック繊維連結部の極低温電子顕微鏡による構造解析2009

    • Author(s)
      Makino, F., Kato, T., Miyata, T., Namba, K.
    • Organizer
      Structural analysis of the flagellar hook-filament junction by electron cryomicroscopy. 日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま/徳島文理大学
    • Year and Date
      2009-10-30
    • Related Report
      2009 Final Research Report
  • [Presentation] 細菌べん毛フック繊維連結部の極低温電子顕微鏡による構造解析.Structural analysis of the flagellar hook-filament junction by electron cryomicrospy.2009

    • Author(s)
      Makino, F., etal
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      アスティとくしま/徳島文理大学
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Electron dose effect on 3D image reconstruction of a biological macromolecular structure by electron cryomicroscopy2009

    • Author(s)
      Ashihara, M., Kato T., Fujii, T., Matsunami H., Miyata T., Namba K.
    • Organizer
      EMBO Course on Image Processing for CryoEM
    • Place of Presentation
      Birkbeck College, London, U.K.
    • Year and Date
      2009-09-10
    • Related Report
      2009 Final Research Report
  • [Presentation] Electron dose effect on 3D image reconstruction of a biological macromolecular structure by electron cryomicrospy.2009

    • Author(s)
      Ashihara, M., etal
    • Organizer
      EMBO Course on Image Processing for CryoEM
    • Place of Presentation
      Birkbeck College, U.K
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] Common architecture between the flagellar export apparatus and F1- ATPase revealed by the structure of FliI and FliJ2009

    • Author(s)
      Ibuki, T., Minamino, T., Miyata, T., Kato, T., Namba, K., Imada, K.
    • Organizer
      International Symposium "Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes"
    • Place of Presentation
      Shirankaikan, Japan
    • Year and Date
      2009-09-08
    • Related Report
      2009 Final Research Report
  • [Presentation] Common architecture between the flagellar export apparatus and F_-ATPase revealed by the structure of Flil and FliJ.2009

    • Author(s)
      Ibuki, T., etal
    • Organizer
      International Symposium "Innovative Nanoscience of Supermolecular Motor Proteins Working in Bio membranes"
    • Place of Presentation
      Shlrankalkan 京都大学
    • Year and Date
      2009-09-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析.2009

    • Author(s)
      宮田知子
    • Organizer
      特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回ワークショップ
    • Place of Presentation
      湘南国際村センター国際会議場
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析2009

    • Author(s)
      宮田知子
    • Organizer
      特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回ワークショップ
    • Place of Presentation
      湘南国際村センター国際会議場
    • Year and Date
      2009-07-27
    • Related Report
      2009 Final Research Report
  • [Presentation] PAX6-SOX2-DNA複合体の塩基配列特異的な構造変化と遺伝子スイッチ機能2008

    • Author(s)
      近藤寿人, 蒲池雄介, 宮田知子, 加藤貴之, 今田勝巳
    • Organizer
      特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回公開シンポジウム
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2008-12-19
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 細菌べん毛基部体の構造解析2008

    • Author(s)
      加藤貴之, 宮田知子, 藤井高志, 松波秀行, 難波啓一
    • Organizer
      特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回公開シンポジウム
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2008-12-18
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析2008

    • Author(s)
      宮田知子, 加藤貴之, 藤井高志, 松波秀行, 難波啓一
    • Organizer
      特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回公開シンポジウム
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2008-12-18
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析2008

    • Author(s)
      Miyata, T., Kato, T., Fujii, T., Matsunami, H., Namba, K.
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-05
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 極低温電子顕微鏡によるべん毛タイプIII蛋白質輸送装置の構造および機能解析2008

    • Author(s)
      Kawamoto, A., Minamino, T., Miyata, T., Kato, T., Namba, K.
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-03
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Optimal conditions for electron cryomicroscopy with liquid helium-cooled specimen stage. Gordon Research Conference on Three Dimensional Electron Microscopy2008

    • Author(s)
      Ashihara, M., Matsunami, H., Miyata, T., Namba, K.
    • Organizer
      II Ciocco Hotel and Resort
    • Place of Presentation
      Lucca (Barga), Italy
    • Related Report
      2009 Final Research Report
  • [Presentation] 細菌べん毛モーター・スイッチ複合体の構造解析2008

    • Author(s)
      宮田知子
    • Organizer
      特定領域研究「生体超分子の構造形成と機能制御の原子機構」第4回ワークショップ
    • Place of Presentation
      淡路夢舞台国際会議場
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Optimal conditions for electron cryomicroscopy with liquid helium-cooled specimen stage2008

    • Author(s)
      Ashihara, M.
    • Organizer
      Gordon Research Conference on Three Dimensional Electron Microscopy
    • Place of Presentation
      II Ciocco Hotel and Resort, Lucca (Barga), Italy
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.fbs.osaka-u.ac.jp/jp/labo/02

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.fbs.osaka-u.ac.jp/labo/09a.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.fbs.osaka-u.ac.jp/labo/09a.html

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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