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Investigation of the DNA recognition mechanisms of sequence-specific LINE

Research Project

Project/Area Number 20770085
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionThe University of Tokushima

Principal Investigator

MAITA Nobuo  The University of Tokushima, 疾患酵素学研究センター, 准教授 (00404046)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsレトロトランスポゾン / X線結晶構造解析
Research Abstract

The final goal of this project was to obtain the crystal structure of LINE-EN : DNA to reveal how LINE-EN to bind to DNA and cleave it, but it was not completed. During the optimization for crystallization, I found that LINE-EN would form a dimer in solution, and that EN alone is not enough to make the stable complex with DNA and necessary a Myb-like domain located just downstream of EN.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (13 results)

All 2011 2010 2008 Other

All Journal Article (10 results) (of which Peer Reviewed: 4 results) Remarks (3 results)

  • [Journal Article]2011

    • Author(s)
      Hashiguchi T, Ose T, Kubota M, Maita N, Kamishikiryo J, Maenaka K, Yanagi Y.
    • Journal Title

      Nat Struct Mol Biol 18

      Pages: 135-141

    • Related Report
      2010 Final Research Report
  • [Journal Article] Structure of the measles virus haemagglutinin bound to its cellular receptor SLAM2011

    • Author(s)
      Hashiguchi T., et al.
    • Journal Title

      Nature Structural & Molecular Biology

      Volume: 18 Pages: 135-141

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2010

    • Author(s)
      Maita N, Nyirenda J, Igura M, Kamishikiryo J, Kohda D.
    • Journal Title

      J Biol Chem 285

      Pages: 4941-4950

    • Related Report
      2010 Final Research Report
  • [Journal Article]2010

    • Author(s)
      Fujiwara K, Maita N, Hosaka H, Ikeda OK, Nakagawa A, Taniguchi H.
    • Journal Title

      J Biol Chem 285

      Pages: 9971-9980

    • Related Report
      2010 Final Research Report
  • [Journal Article]2010

    • Author(s)
      Ikeda OK, Hosaka H, Maita N, Fujiwara K, Yoshizawa AC, Nakagawa A, Taniguchi H.
    • Journal Title

      J Biol Chem 285

      Pages: 18684-18692

    • Related Report
      2010 Final Research Report
  • [Journal Article] Crystal structure of aminomethyltransferase in complex with dihydrolipoyl-H-protein of the glycine cleavage system2010

    • Author(s)
      Okamura-Ikeda K., et al.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 285 Pages: 18684-18692

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative structural biology of Eubacterial and Archaeal oligosaccharyltransferases2010

    • Author(s)
      Maita N., et al.
    • Journal Title

      The Journal of Biological Chemistry 285

      Pages: 4941-4950

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Global conformational change associated with the two-step reaction catalyzed by Escherichia coli lipoate-protein ligase A2010

    • Author(s)
      Fujiwara K., et al.
    • Journal Title

      The Journal of Biological Chemistry 285

      Pages: 9971-9980

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2008

    • Author(s)
      Hashiguchi T, Kajikawa M, Maita N, Takeda M, Kuroki K, Sasaki K, Kohda D, Yanagi Y,Maenaka K.
    • Journal Title

      J Virol Methods 149

      Pages: 171-174

    • Related Report
      2010 Final Research Report
  • [Journal Article]2008

    • Author(s)
      Igura M, Maita N, Kamishikiryo J, YamadaM, Obita T, Maenaka K, Kohda D.
    • Journal Title

      EMBO J 27

      Pages: 234-243

    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://mms1.ier.tokushima-u.ac.jp/article/0017777.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://mms1.ier.tokushima-u.ac.jp/article/0017777.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://mms1.ier.tokushima-u.ac.jp/article/0017777.html

    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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