Function analysis of Hair keratin in mammary gland

• Project/Area Number: 20770096
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Hirosaki University
• Principal Investigator: NANASHIMA Naoki Hirosaki University, 大学院・保健学研究科, 助教 (80333730)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞骨格 / Hair keratin / 乳腺

Research Abstract

Because Hair keratin genes deleted rat HHR show mammary gland developmental abnormality, I analyzed function of Hair keratin in mammary gland. Hair keratin Kb21 gene expressed in mammary gland in wild SD rat, and it was suggested that Kb21 bound to proteins such as ribosomal protein. Because these proteins could not interact with deleted Kb21 protein, it was suggested that proteins synthesis decreased at lactation in HHR.

Research Products

• [Journal Article] (2010)
  • Author(s): Shimizu Takeshi, Fan Yang, Yamana Daisuke, Miura Takuya, Nanashima Naoki, Yamada Toshiyuki, Tsuchida Shigeki
  • Journal Title: Cancer Sci. 101 Pages: 1093-1098
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Asano Jumpei, Kudo Toshihiro, Shimizu Takeshi, Fan Yang, Nanashima Naoki, Yamana Daisuke, Miura Takuya, Yamada Toshiyuki, Tsuchida Shigeki
  • Journal Title: Cancer Sci. 101 Pages: 869-875
  • Peer Reviewed
• [Journal Article] Histone acetylation and steroid receptor coactivator expression during clofibrate-induced rat hepatocarcinogenesis (2010)
  • Author(s): Asano J, Kudo T, Shimizu T, Fan Y, Nanashima N, Yamana D, Miura T, Yamada T, Tsuchida S.
  • Journal Title: Cancer Science 101 Pages: 869-875
  • NAID: 10026590615
  • Peer Reviewed
• [Journal Article] Glutathione S-transferase A4 is a positive marker for rat he patio foci induced by clofibrate and genotoxic carcinogens (2010)
  • Author(s): Shimizu T, Fan Y, Yamana D, Miura T, Nanashima N, Yamada T, Tsuchida S.
  • Journal Title: Cancer Science 101 Pages: 1093-1098
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Akita Miki, Nanashima Naoki, Yamada Toshiyuki Nakano, Hajime, Shimizu Takeshi, Fan Yang, Tsuchida Shigeki
  • Journal Title: Hirosaki Med. J. 60 Pages: 1-11
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Masanori Horie, Keiko Nishio, Katsuhide Fujita, Shigehisa Endoh, Arisa Miyauchi, Yoshiro Saito, Hitoshi Iwahashi, Kazuhiro Yamamoto, Hideki Murayama, Hajime Nakano, Naoki Nanashima, Etsuo Niki, Yasukazu Yoshida
  • Journal Title: Chem. Res. Toxicol. 22 Pages: 543-553
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Yang Fan, Toshiyuki Yamada, Takeshi Shimizu, Naoki Nanashima, Miki Akita, Kohji Suto, Shigeki Tsuchida
  • Journal Title: Toxicol. Pathol. 37 Pages: 209-217
  • Peer Reviewed
• [Journal Article] Physical and functional interactions between hematopoietic cell-specific ETS transcription factors and homeodomain proteins (2009)
  • Author(s): Toshiyuki Yamada
  • Journal Title: Leukemia Research 33 Pages: 483-9
  • Peer Reviewed
• [Journal Article] (2008)
  • Author(s): Toshiyuki Yamada, Takeshi Shimizu, Takuya Sakurai, Naoki Nanashima, Fumiko Kihara-Negishi, Mitsuhiro Suzuki, Yang Fan, Miki Akita, Tsuneyuki Oikawa, Shigeki Tsuchida
  • Journal Title: Leuc. Res. 33 Pages: 483-489
  • Peer Reviewed
• [Journal Article] (2008)
  • Author(s): Naoki Nanashima, Miki Akita, Toshiyuki Yamada, Takeshi Shimizu, Hajime Nakano, Yang Fan, Shigeki Tsuchida
  • Journal Title: J. Biol. Chem. 283 Pages: 16868-16875
  • Peer Reviewed
• [Journal Article] The hairless phenotype of the Hirosaki hairless rat is due to the deletion of an 80-kb genomic DNA containing five basic keratin genes (2008)
  • Author(s): Naoki Nanashima
  • Journal Title: Journal of Biological Chemistry 283 Pages: 16868-75
  • Peer Reviewed
• [Journal Article] Development of glutathione S-transferase-P-negative foci accompanying nuclear factor-erythroid 2-related factor 2 expression during early stage of rat hepatocarcinogenesis (2008)
  • Author(s): Fan Yang
  • Journal Title: Cancer Science 99 Pages: 497-501
  • Peer Reviewed
• [Presentation] Expression of Hair keratin Kb21 and interaction for ribosomal protein in rat mammary gland (2009)
  • Author(s): Naoki Nanashima, Toshiyuki Yamada, Takeshi Shimizu, Takuya Miura, Daisuke Yamana, Shigeki Tsuchida
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] ラット乳腺における Hair keratin の発現とリボソームへの結合 (2009)
  • Author(s): 七島直樹
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2009-10-22
• [Presentation] Deletion of phospholipase A2 group IV c gene and altered steroid hormone metabolism in Hirosaki hairless rat (2009)
  • Author(s): Naoki Nanashima, Toshiyuki Yamada, Takeshi Shimizu, Takuya Miura, Daisuke Yamana, Shigeki Tsuchida
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-02
• [Presentation] The hairless phenotype of the Hirosaki Hairless Rat is due to the deletion of an 80-kb genomic DNA containing five basic keratin genes and the expression of fusion gene (2008)
  • Author(s): Naoki Nanashima, Miki Akita, Toshiyuki Yamada, Takeshi Shimizu, Yang Fan, Shigeki Tsuchida
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] STAT5A Modified with O-Linked N-Acetylglucosamine (O-GlcNAc ) induces apoptosis in the Mammary Gland (2008)
  • Author(s): Naoki Nanashima, Toshiyuki Yamada, Takeshi Shimizu, Miki Akita, Yang Fan, Shigeki Tsuchida
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] 遺伝性のHirosaki hairless ratにおける塩基性ケラチン遺伝子群の欠失と融合遺伝子の発現 (2008)
  • Author(s): 七島直樹、秋田美季、山田俊幸、清水武史、中野創、范洋、葛西宏介、土田成紀
  • Organizer: 日本生化学東北支部第74回例会・シンポジウム
  • Place of Presentation: アイーナいわて県民情報交流センター
  • Year and Date: 2008-05-17
• [Presentation] 遺伝性のHirosaki hairless ratにおける塩基性ケラチン遺伝子群の欠失と融合遺伝子の発現 (2008)
  • Author(s): 七島直樹
  • Organizer: 日本生化学会東北支部会74回例会
  • Place of Presentation: 岩手県民情報交流センター
  • Year and Date: 2008-05-17