Analysis on the signal transduction pathway by the reconstitution of signaling components

Research Project

Project/Area Number	20770098
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Functional biochemistry
Research Institution	The University of Tokyo
Principal Investigator	MIYADERA Hiroko The University of Tokyo, 大学院・医学系研究科, 助教 (40361464)
Project Period (FY)	2008 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywords	細胞情報伝達機構 / シグナル伝達
Research Abstract	Tumor necrosis factor (TNF)-related apoptosis-inducing ligand/Apoptosis ligand 2 (TRAIL/Apo2L) induces tumor cell specific apoptosis. In the extracellular domain of TRAILR1, there are two SNPs (H141R and R209T) which were reported to be associated with cancers. We expressed and refolded TRAIL and TRAILR1 recombinant protein in E. coli in order to assess the effects of these polymorphisms to the ligand-receptor interaction.