Study of functional and physical interactions between an amino acid transporter complex LAT1-4F2hc and Integrin by using Reconstituted proteoliposomes

• Project/Area Number: 20770101
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: NAGAMORI Shushi Osaka University, 医学系研究科, 助教 (90467572)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: トランスポーター / プロテオリポソーム / 膜複合体 / ガン

Research Abstract

LAT1(L-type amino acid transporter 1) is one of the transporters which uptake essential amino acids. Expression of the function requires an interacting partner, CD98/4F2hc. In this study, a new experimental system has been developed to study LAT1-function in detail and it is shown that CD98/4F2hc is not essential for the function. Furthermore, Annexine II was identified as a CD98/4F2hc -binding protein beside integrin.

Research Products

• [Journal Article] A novel transporter of SLC22 family specifically transports prostaglandins and co-localizes with 15- hydroxylprostaglandindehydrogenase in renal proximal tubules (2010)
  • Author(s): Shiraya K, Hirata T, Hatano R, Nagamori S, Wiriyasermkul P, Jutabha P, Matsubara M, Muto S, Tanaka H, Asano S, Anzai N, Endou H, Yamada A, Sakurai H, Kanai Y.
  • Journal Title: J Biol Chem.
  • Peer Reviewed
• [Journal Article] System L amino acid transporter inhibitor enhances anti-tumor activity of cisplatin in a head and neck squamous cell carcinoma cell line (2009)
  • Author(s): Yamauchi K, Sakurai H, Kimura T, Wiriyaserm- kul P, Nagamori S, Kanai Y, Kohno N.
  • Journal Title: Cancer Lett. 276 Pages: 95-101
  • Peer Reviewed
• [Journal Article] A novel role of the C-terminus of b0, +AT in the ER-Golgi trafficking of the rBAT-b0, +AT heterodimeric amino acid transporter (2009)
  • Author(s): Sakamoto S, Chairoungdua A, Nagamori S, Wiriyasermkul P, Promchan K, Tanaka H, Kimura T, Ueda T, Fujimura M, Shigeta Y, Naya Y, Akakura K, Ito H, Endou H, Ichikawa T, Kanai Y.
  • Journal Title: Biochem J. 417 Pages: 441-448
  • Peer Reviewed
• [Journal Article] System L Amino Acid Transporter Inhibitor Enhances Antitumor Activity of Cisplatin in a Head and Neck Squamous Cell Carcinoma cell line (2009)
  • Author(s): Yamauchi, 他
  • Journal Title: Cancer Lett 276 Pages: 95-101
  • Peer Reviewed
• [Journal Article] A novel role of C-terminus of b^<0,+>AT in the ER-Golgi trafficking of rBAT-b^<0,+>AT heterodimeric amino acid transporter. (2009)
  • Author(s): Sakamoto, 他
  • Journal Title: Biochem J 15 Pages: 441-448
  • Peer Reviewed
• [Journal Article] Mutations in glucose transporter 9 gene SLC2A9 cause renal hypouricemia (2008)
  • Author(s): Matsuo H, Chiba T, Nagamori S, Nakayama A, Domoto H, Phetdee K, Wiriyasermkul P, Kikuchi Y, Oda T, Nishiyama J, Nakamura T, Morimoto Y, Kamakura K, Sakurai Y, Nonoyama S, Kanai Y, Shinomiya N.
  • Journal Title: Am J Hum Genet. 83 Pages: 744-751
  • Peer Reviewed
• [Journal Article] L-glutamate enhances methylmercury toxicity by synergistically increasing oxidative stress (2008)
  • Author(s): Amonpatumrat S, Sakurai H, Wiriyaserm- kul P, Khunweeraphong N, Nagamori S, Tanaka H, Piyachaturawat P, Kanai Y.
  • Journal Title: J Pharmacol Sci. 108 Pages: 280-289
  • NAID: 10024593004
  • Peer Reviewed
• [Journal Article] Mutations in Glucose Transporter 9 Gene SLC2A9 Cause Renal Hypouricemia (2008)
  • Author(s): Matsuo, 他
  • Journal Title: Am J Hum Genet 83 Pages: 744-751
  • Peer Reviewed
• [Journal Article] L-Glutamate enhances methylmercury toxicity by synergistically increasing oxidative stress (2008)
  • Author(s): Amonpatumlat, 他
  • Journal Title: J Pharmacol Sci 108 Pages: 280-289
  • Peer Reviewed
• [Presentation] 癌特異的アミノ酸トランスポーターLAT1を介したPETリガンドL-[3-F]-α-methyltyrosine(FAMT)の輸送キネティクス (2010)
  • Author(s): 永森収志, 他
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪府・大阪市
  • Year and Date: 2010-03-18
• [Presentation] 癌特異的アミノ酸トランスポーターLAT1を介したPETリガンドL[3-F]-α-methyltyrosine (FAMT)の輸送キネティクス (2010)
  • Author(s): 永森牧志, 他
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪府・大阪市
  • Year and Date: 2010-03-18
• [Presentation] The substrate recognition for PET ligands by L-type amino acid transporter 1 (LAT1) (2009)
  • Author(s): Pattama Wiriyasermkul, 永森収志, 他
  • Organizer: 第32回日本分子生物学学会
  • Place of Presentation: 神奈川県、横浜市
  • Year and Date: 2009-12-12
• [Presentation] メタボロミクスを用いた新規有機アニオントランスポーターOatn1の生体内における機能の解析 (2009)
  • Author(s): 永森収志, 他
  • Organizer: 第116回日本薬理学会近畿部会
  • Place of Presentation: 滋賀県・大津市
  • Year and Date: 2009-11-13
• [Presentation] メタボロミクスを用いた新規有機アニオントランスポーターOatnlの生体内における機能の解析 (2009)
  • Author(s): 永森牧志, 他
  • Organizer: 第116回日本薬理学会近畿部会
  • Place of Presentation: 滋賀県・大津市
  • Year and Date: 2009-11-13
• [Presentation] Characterization of substrate recognition site in L-type Amino acid transporter 1 (LAT1) (2009)
  • Author(s): Pattama Wiriyasermkul, 永森収志, 他
  • Organizer: The 36th congress of International Union of Physiological Sciences
  • Place of Presentation: 京都府・京都市
  • Year and Date: 2009-07-31
• [Presentation] Annexine A2- P11 Complex Interacts with CD98hc (2009)
  • Author(s): K. Promchan, K. Takafuji, T. Kimura, S. Nagamori, Y. Kanai
  • Organizer: The 36th congress of International Union of Physiological Sciences
  • Place of Presentation: 京都府・京都市
  • Year and Date: 2009-07-31
• [Presentation] Characterization of an orphan transporter in SLC 7 family (2009)
  • Author(s): 永森収志, 他
  • Organizer: The 36th congress of International Union of Physiological Sciences
  • Place of Presentation: 京都府・京都市
  • Year and Date: 2009-07-31
• [Presentation] Characterization of an orphan transporter in SLC 7 family (2009)
  • Author(s): 永森牧志, 他
  • Organizer: The 36^<th> congress of International Union of Physiological Sciences
  • Place of Presentation: 京都府・京都市
  • Year and Date: 2009-07-31
• [Presentation] 腫瘍細胞型アミノ酸トランスポーターLAT1を介したPETリガンドL-[3-F]-α-methyltyrosine(FAMT)の輸送キネティクス (2009)
  • Author(s): 永森収志, 他
  • Organizer: 第115回日本薬理学会近畿部会
  • Place of Presentation: 石川県・金沢市
  • Year and Date: 2009-06-26
• [Presentation] 腫瘍細胞型アミノ酸トランスポーターLAT1を介したPETリガンドL-[3-F]-α-methyltyrosine (FAMT)の輸送キネティクス (2009)
  • Author(s): 永森牧志, 他
  • Organizer: 第115回日本薬理学会近畿部会
  • Place of Presentation: 石川県・金沢市
  • Year and Date: 2009-06-26
• [Presentation] Substrate recognition of L-type amino acid transporter 1, LAT1, and transport kinetics of its PET ligand L-[3-F]-α-methyltyrosine, FAMT (2009)
  • Author(s): 永森収志, 他
  • Organizer: Gordon Research Conference: Mechanisms Of Membrane Transport
  • Place of Presentation: 米国・メイン州ウオータービル
  • Year and Date: 2009-06-15
• [Presentation] Substrate recognition of L-type amino acid transporter 1, LATI, and transport kinetics of its PET ligand L-[3-F]-α-methyltyrosine, EAMT (2009)
  • Author(s): 永森牧志, 他
  • Organizer: Gordon Research Conference : Mechanisms Of Membrane Transport
  • Place of Presentation: 米国・メイン州ウオータービル
  • Year and Date: 2009-06-15
• [Presentation] C. elegans amino acid transporter-6 (AAT-6), which does not possess a conserved cysteine, requires non-SS interaction with glyco - protein ATG-1 for the function (2008)
  • Author(s): 永森収志, 他
  • Organizer: Gordon Research Conference Membrane Transport. Gordon Research Conference Membrane Transport
  • Place of Presentation: イタリアLucca
  • Year and Date: 2008-07-22
• [Presentation] C. elegans amino acid transporter-6 (AAT-6), which does not possess aconserved cysteine, requires non-SS interaction with glycoprotein ATG-1 for the function (2008)
  • Author(s): 永森牧志, 他
  • Organizer: Gordon Research Conference Membrane Transport
  • Place of Presentation: イタリア・ルッカ
  • Year and Date: 2008-07-22