Structural Insight for Ca channel Regulation by Calmodulin

• Project/Area Number: 20770110
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Fukuoka University
• Principal Investigator: MORI Masayuki Fukuoka University, 医学部, 講師 (80342640)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 生理学 / シグナル伝達 / カルシウム / イメージング / 構造 / 一般生理 / 細胞内カルシウム / イオンチャネル / カルシウム結合蛋白質 / X線構造解析 / 興奮性細胞 / 蛍光エネルギー移動(FRET)

Research Abstract

The overall aim of the proposed studies was to understand the mechanism of activation, inhibition and regulations of Ca channels by Calcium-binding protein Calmodulin (CaM). The primary focus was structural determination of the calcium free form apoCaM and Channel IQ domain complex. Until today, we have a couple of crystals, those will be needed to check reproducibility and their structure. For the imaging of Ca^<2+> and CaM- channel interaction, we have succeeded to develop new system combined with Fura-2 and FRET. By using this system we could establish Ca^<2+> titrative experiment in vivo with high fidelity. We hope that this approach will be often to use in the field wide range of the physiological science to reveal Ca^<2+> related molecular dynamics.

Research Products

• [Journal Article] 森誠之心血管 (2009)
  • Author(s): 井上隆司、瓦林靖広
  • Journal Title: TRPチャネルを巡る病態生理の新展開 (29) Pages: 11-21
• [Journal Article] 菅忠受容体機械刺激協働による心血管Ca2+流入チャネルTRPC6の活性化増幅機構 (2009)
  • Author(s): 井上隆司、森誠之、瓦林靖広
  • Journal Title: 日本薬理学雑誌 134 Pages: 116-121
  • Peer Reviewed
• [Journal Article] Synergistic activation of vascular TRPC6 channel by receptor and mechanical stimulation via phospholipaseC/diacylglycerol and phospholipase A2/omega-hydroxylase/20-HETE pathways (2009)
  • Author(s): Inoue R, Jensen LJ, Jian Z, Shi J, Hai L, Lurie AI, Henriksen FH, Salomonsson M, Morita H. Kawarabayashi Y, Mori M, Mori Y, Ito Y.
  • Journal Title: Circ Res. 104 Pages: 1399-409
  • Peer Reviewed
• [Journal Article] Synergistic activation of vascular TRPC6 channel by receptor and mechanical stimulation via phospholipase C/diacylglycerol and phospholipase A2/omega-hydroxylase/20-HETE pathways (2009)
  • Author(s): 井上隆司 et al.
  • Journal Title: Circulation Research 104 Pages: 1399-1409
  • Peer Reviewed
• [Journal Article] 心血管TRPチャネルを巡る病態生理の新展開 (2009)
  • Author(s): 井上隆司、瓦林靖広、森誠之
  • Journal Title: 心電図 29 Pages: 11-21
  • NAID: 10026293324
• [Journal Article] 受容体機械刺激協働による心血管Ca2+流入チャネルTRPC6の活性化増幅機構 (2009)
  • Author(s): 井上隆司、森誠之、瓦林靖広、菅忠
  • Journal Title: 日本薬理学雑誌 134 Pages: 116-121
• [Journal Article] Nitric oxide cGMP protein kinase G pathway negatively regulates vascular transient receptor potential channel TRPC6 (2008)
  • Author(s): Takahashi S, Lin H, Geshi N, Mori Y, Kawarabayashi Y, Takami N, Mori MX, Honda A, Inoue R.
  • Journal Title: J Physiol. 586 Pages: 4209-4223
  • Peer Reviewed
• [Journal Article] Crystal structure of the CaV2 IQ domain in complex with Ca2+/calmodulin : high-resolution mechanistic implications for channel regulation by Ca2+ (2008)
  • Author(s): Mori M. et.al.
  • Journal Title: Structure 16 Pages: 607-620
  • Peer Reviewed
• [Journal Article] Nitric oxide-cGMP-protein kinase G pathway negatively regulates vascular transient receptor potential channel TRPC6 (2008)
  • Author(s): Takahashi S., et.al.
  • Journal Title: J. Physiology 586 Pages: 4209-4223
  • Peer Reviewed
• [Presentation] 細胞内カルシウムとCa2+チャネル-Calmodulin作用の準同時的イメージング西 (2009)
  • Author(s): 森誠之
  • Organizer: 日本生理学会
  • Place of Presentation: 福岡県福岡市福岡県歯科医師会館
  • Year and Date: 2009-11-07
• [Presentation] 細胞内カルシウムとCa2+チャネル-Calmodulin 作用の準同時的イメージング (2009)
  • Author(s): 森誠之
  • Organizer: 西日本生理学会
  • Place of Presentation: 福岡県 福岡市 福岡県歯科医師会館
  • Year and Date: 2009-11-07
• [Presentation] カルシウムとCaチャネル-カルモジュリン相互作用の定量的同時イメージング (2009)
  • Author(s): 森誠之
  • Organizer: 日本生化学会年会
  • Place of Presentation: 兵庫県神戸市神戸国際会議場
  • Year and Date: 2009-10-24
• [Presentation] カルシウムとCaチャネル-カルモジュリン相互作用の定量的同時イメージング (2009)
  • Author(s): 森誠之
  • Organizer: 日本生化学会 年会
  • Place of Presentation: 兵庫県 神戸市 神戸国際会議場
  • Year and Date: 2009-10-24
• [Presentation] Fundamental molecular mechanism of TRPC6 channel with Calmodulin interaction (2009)
  • Author(s): 森誠之
  • Organizer: 国際生理学会
  • Place of Presentation: 京都府京都市京都国際会議場
  • Year and Date: 2009-06-27
• [Presentation] Fundamental molecular mechanism of TRPC6 channel with Calmodulin interaction (2009)
  • Author(s): 森誠之
  • Organizer: 国際生理学会
  • Place of Presentation: 京都府 京都市 京都国際会議場
  • Year and Date: 2009-06-27
• [Presentation] Structural basis for calmodulin mediated Ca2+ entry channel国際シンポジウム (2009)
  • Author(s): 森誠之
  • Organizer: 国際生理学会
  • Place of Presentation: 愛知県名古屋市名古屋市立大学
  • Year and Date: 2009-06-24
• [Presentation] Structural basis for calmodulin-mediated Ca2+ entry channel (2009)
  • Author(s): 森誠之
  • Organizer: 国際シンポジウム 国際生理学会
  • Place of Presentation: 愛知県 名古屋市 名古屋市立大学
  • Year and Date: 2009-06-24
• [Presentation] Atomic structure of Ca,2 IQ domain complexed with Ca^<2+>/calmodulin (2008)
  • Author(s): Mori M., et.al.
  • Organizer: Society for Neuroscience
  • Place of Presentation: Washington D.C. (U.S.A.)(Neuroscience meeting)
  • Year and Date: 2008-11-17
• [Remarks]
  • URL: http://www.med.fukuoka-u.ac.jp/physiol/staff/staff2.htm
• [Remarks]
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• [Remarks]
  • URL: http://www.med.fukuoka-u.ac.jp/physiol/staff/staff2.htm
• [Remarks]
  • URL: http://resweb2.jhk.adm.fukuoka-u.ac.jp/FukuokaUniv/R101J_Action.do
• [Remarks]
  • URL: http://www.med.fukuoka-u.ac.jp/physiol/index-j.htm