Development of caspase-3 indicator based-on bioluminescence-fluorescence double resonant energy transfer
| Project/Area Number |
20770115
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Hokkaido University |
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Principal Investigator |
SAITO Kenta Hokkaido University, 電子科学研究所, 特任准教授 (60374659)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | バイオイメージング / 細胞内情報伝達 / 生物発光共鳴エネルギー移動 / 機能イメージング / カルシウム指示薬 / BRET / イメージング / Rluc / Venus / 発光タンパク / 蛍光タンパク質 / 自家蛍光 |
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| Research Abstract |
In this study, first, bioluminescence resonance energy transfer (BRET) based Ca^<2+> indicator, BRAC was developed and tested to evaluate its physiochemical properties. Second, BRAC was used to visualize Ca^<2+> dynamics in living cells (HeLa cells) and living plant (Arabidopsis thaliana) leaf. Finally, to make the indicator based on bioluminescent-fluorescent double resonant energy transfer, appropriate FRET acceptor for FRET donor Venus were investigated in possible orange to red fluorescent protein candidates. However FRET efficiency between Venus and these orange to red fluorescent proteins were poor, so additional studies should be needed.
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Report
(4 results)
Research Products
(9 results)
