Study of regulatory mechanism of intracellular vesicle transport by motor proteins using 3-dimensional tracking of nano-particle.

• Project/Area Number: 20770116
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: TAKETOSHI Kambara The University of Tokyo, 大学院・理学系研究科, 特任研究員 (40451637)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: モータータンパク質 / 細胞内輸送

Research Abstract

Motor proteins play an important role in transporting organelles and vesicles to the destination. The goal of this project is to construct new system to understand the regulatory mechanism of motor-based intracellular transport. I purified recombinant motor proteins, and could obtain fluorescent nano-particles attached to these proteins. The nano-particles were induced to semi-intact cells, and I could successfully track the 3-dimensional movement of these in high speciotemporal resolution.