Functional analysis of structural proteins of the axonemal inter-doublet links

• Project/Area Number: 20770119
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: YANAGISAWA Haruaki The University of Tokyo, 大学院・理学系研究科 (70466803)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 細胞運動 / 鞭毛・繊毛 / 生体分子 / 生物物理

Research Abstract

The outer doublet microtubules in ciliary and flagellar axonemes are connected with each other by elastic links called the interdoublet (nexin) links. This structures are important for maintenance of axonemal structure and converting sliding between doublets into bending. The link has been found in early electron microscopic studies, but little is known about its molecular composition. In this study I cloned putative nexin components from partially extracted axonemes, in which outer doublets remained connected together. Localization and mutational analyses confirmed the involvement of these components in the nexin links.

Research Products

• [Journal Article] Tubulin polyglutamylation regulates axonemal motility by modulating activities of inner-arm dyneins. (2010)
  • Author(s): Kubo T, Yanagisawa HA, Yagi T, Hirono M, Kamiya R.
  • Journal Title: Current Biology. 20巻 Pages: 441-445
  • Peer Reviewed
• [Journal Article] Tubulin Polyglutamylation Regulates Axonemal Motility by Modulating Activities of Inner-Arm Dyneins (2010)
  • Author(s): Tomohiro Kubo, Haru-aki Yanagisawa, Toshiki Yagi, Masafumi Hirono, Ritsu Kamiya
  • Journal Title: Current Biology Volume: 20 Pages: 441-445
  • Peer Reviewed
• [Journal Article] IC97 is a novel intermediate chain of I1 dynein that interacts with tubulin and regulates interdoublet sliding. (2009)
  • Author(s): Wirschell M, Yang C, Yang P, Fox L, Yanagisawa HA, Kamiya R, Witman GB, Porter ME, Sale WS.
  • Journal Title: Molecular Biology of the Cell. 20巻 Pages: 3044-3054
  • Peer Reviewed
• [Journal Article] Systematic comparison of in vitro motile properties between Chlamydomonas wild-type and mutant outer arm dyneins each lacking one of the three heavy chains. (2009)
  • Author(s): Furuta A, Yagi T, Yanagisawa HA, Higuchi H, Kamiya R.
  • Journal Title: Journal of Biological Chemistry. 284巻 Pages: 5927-5935
  • Peer Reviewed
• [Journal Article] IC97 Is a Novel Intermediate Chain of Il Dynein That Interacts with Tubulin and Regulates Interdoublet Sliding (2009)
  • Author(s): Maureen Wirschell, Chun Yang, Pinfen Yang, Laura Fox, Haru-aki Yanagisawa, Ritsu Kamiya, George B.Witman, Mary E.Porter, Winfield S.Sale
  • Journal Title: Molecular Biology of the Cell Volume: 20 Pages: 3044-3054
  • Peer Reviewed
• [Presentation] Mutants that lack PACRG, a highly conserved axonemal protein, produce flagella defective in intra-doublet structures (2008)
  • Author(s): Haruaki Yanagisawa, Ritsu Kamiya
  • Organizer: 13th International Chlamydomonas Conference
  • Place of Presentation: Hyeres-les-Palmiers, France
• [Presentation] Mutants that lack PACRG, a highly conserved axonemal protein, produce flagella defective in intra-doublet structures (2008)
  • Author(s): Haruaki Yanagisawa, Ritsu Kamiya
  • Organizer: 13^<th> International Chlamydomonas Conference
  • Place of Presentation: Hyeres-les-Palmiers, France