The role of mitochondrial proteins involved in the innate immunity

• Project/Area Number: 20770123
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Kyushu University
• Principal Investigator: KOSHIBA Takumi Kyushu University, 理学研究院, 准教授 (70403970)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ミトコンドリア / RNAウイルス / 自然免疫 / シグナル伝達 / インターフェロン / 抗ウイルス免疫 / MAVS / Mitofusin 2 / インターフェロンβ / 蛋白質科学

Research Abstract

Innate cellular immune response to viral infection results in the activation of multiple signaling steps, ultimately regulates the expression of type-I interferons (IFNs), and a mitochondrial membrane protein, MAVS plays an important role in the pathway. In the presence study, we found that mitofusin 2 (Mfn2), a mediator of mitochondrial fusion, cooperates with MAVS, and participates in the antiviral immunity. Our results suggest that Mfn2 acts as a negative regulator for antiviral signaling, and would have a distinct function unrelated to mitochondrial dynamics.

Research Products

• [Journal Article] ミトコンドリア外膜タンパク質Mitofusin 2によるウイルス免疫制御機構. (2010)
  • Author(s): *小柴琢己
  • Journal Title: 実験医学(羊土社) 28 Pages: 429-432
• [Journal Article] ミトコンドリア外膜上でのウイルス免疫制御機構. (2010)
  • Author(s): *小柴琢己
  • Journal Title: 生化学(日本生化学会) 82 Pages: 135-139
  • NAID: 10026300531
• [Journal Article] ミトコンドリア外膜タンパク質Mitofusin2によるウイルス免疫制御機構 (2010)
  • Author(s): 小柴琢己
  • Journal Title: 実験医学(羊土社) 28 Pages: 429-432
• [Journal Article] ミトコンドリア外膜上でのウイルス免疫制御機構. (2010)
  • Author(s): 小柴琢己
  • Journal Title: 生化学(日本生化学会) 82 Pages: 135-139
  • NAID: 10026300531
• [Journal Article] Mitofusin 2 inhibits mitochondrial antiviral signaling. (2009)
  • Author(s): Yasukawa, K., Oshiumi, H., Takeda, M., Ishihara, N., Yanagi, Y., Seya, T., Kawabata, S., *Koshiba, T.
  • Journal Title: Science Signaling 2
• [Journal Article] Mitofusin 2 inhibits mitochondrial antiviral signaling. (2009)
  • Author(s): Yasukawa, et al.
  • Journal Title: Science Signaling 2
  • Peer Reviewed
• [Journal Article] Factor C acts as a lipopolysaccharide-responsive C3 convertase in hor seshoe crab complement activation (2008)
  • Author(s): Ariki, et.al.
  • Journal Title: J. Immunol 181 Pages: 7994-8001
  • Peer Reviewed
• [Presentation] Mitochondrial dynamics in mammalian cells and an insight for antiviral innate immunity (2010)
  • Author(s): Takumi Koshiba
  • Organizer: STINT workshop on Invertebrates
  • Place of Presentation: ウプサラ市(スウェーデン)
  • Year and Date: 2010-03-15
• [Presentation] Mitochondrial dynamics and an insight for antiviral innate immunity (2010)
  • Author(s): Takumi Koshiba
  • Organizer: International Symposium "Intercellular Recognition and Allogenic Authentication : Perspectives of Reproduction Mechanisms Shared by Animals and Plants"
  • Place of Presentation: SIR Winston Hotel名古屋市
  • Year and Date: 2010-01-14
• [Presentation] Mitochondrial dynamics in mammalian cells and an insight for antiviral innate immunity (2010)
  • Author(s): Koshiba, T.
  • Organizer: STINT workshop on Invertebrates (Uppsala University, Sweden
• [Presentation] Mitochondrial dynamics and an insight for antiviral innate immunity (2010)
  • Author(s): Koshiba, T.
  • Organizer: International Symposium "Intercellular Recognition and Allogenic Authentication: Perspectives of Reproduction Mechanisms Shared by Animals and Plants"
  • Place of Presentation: SIR Winston Hotel (Nagoya, Japan)
• [Presentation] Mitofusin 2 acts as an inhibitor of mitochondrial antiviral signaling (2009)
  • Author(s): 小柴琢己
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] ミトコンドリア外膜上での自然免疫調節機構 (2009)
  • Author(s): 小柴琢己
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本全日空ホテルニュースカイ
  • Year and Date: 2009-05-21
• [Presentation] Mitofusin 2 acts as an inhibitor of mitochondrial antiviral signaling (2009)
  • Author(s): 小柴琢己
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
• [Presentation] ミトコンドリア外膜上での自然免疫調節機構 (2009)
  • Author(s): 小柴琢己
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本全日空ホテルニュースカイ(熊本)
• [Presentation] Mitofusin 2 acts as a negative regulator of mitochondrial antiviral immunity (2009)
  • Author(s): Yasukawa, K., Oshiumi, H., Takeda, M., Ishihara, N., Yanagi, Y., Seya, T., Kawabata, S., Koshiba, T.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] Mitofusin 2 acts as a negative regulator of mitochondrial antiviral immunity (2009)
  • Author(s): Yasukawa, K., Oshiumi, H., Takeda, M., Ishihara, N., Yanagi, Y., Seya, T., Kawabata, S., Koshiba, T.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: バシフィコ横浜
• [Presentation] 自然免疫に関わるミトコンドリア膜蛋白質MAVS機能ドメインの大腸菌による大量発現系の構築 (2008)
  • Author(s): 安川開、川畑俊一郎、小柴琢己
  • Organizer: BMB2008
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] 自然免疫に関わるミトコンドリア膜蛋白質MAVS機能ドメインの大腸菌による大量発現系構築 (2008)
  • Author(s): 安川開、川畑俊一郎、小柴琢己
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(神戸)
• [Remarks]
  • URL: http://www.biology.kyushu-u.ac.jp/~koshiba/
• [Remarks]
  • URL: http://www.biology.kyushu-u.ac.jp/~koshiba/