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Development of a Time-Resolved Infrared Spectrophotometer ~ Elucidation of the proton pumping mechanism ~

Research Project

Project/Area Number 20770125
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionUniversity of Hyogo

Principal Investigator

YAMAGUCHI Satoru  University of Hyogo, 大学院・生命理学研究科, 助教 (20347529)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords時間分解測定 / 赤外分光 / プロトンポンプ / 赤外分光法 / マイクロチップ / 生体エネルギー変換 / 構造生物学 / 膜蛋白質 / エネルギー変換
Research Abstract

A femtosecond infrared laser was combined to our infrared spectrometer for light source. A chamber was constructed to cover while optical system because the water vapor makes IR spectra worth remarkably and it was filled with dry N_2 gas. For time-resolved IR experiment, a micro flow cell that dimension has 50 x 10^<-6> m (light path length) and 400 x 10^<-6> m (flow pass width) was made by sandwiched SU-8 polymer between CaF_2 windows. A difference spectra between reduced state and oxidized state of cytochrome c oxidase was acquired by using these system.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (6 results)

All 2009 2008

All Journal Article (6 results) (of which Peer Reviewed: 1 results)

  • [Journal Article] Participation of the BC loop in the correct folding of bacteriorhodopsin as revealed by solid-state NMR2009

    • Author(s)
      I. Kawamura, J. Tanabe, M. Ohmine, S. Yamaguchi, S. Tuzi, A. Naito
    • Journal Title

      Photochemistry and Photobiology 85

      Pages: 624-630

    • Related Report
      2009 Final Research Report
  • [Journal Article] Spectroscopic Characterization of a Hydroperoxo-Heme Intermediate: Conversion of a Side-On Peroxo to an End-On Hydroperoxo2009

    • Author(s)
      J.G. Liu, T. Ohta, S. Yamaguchi, T. Ogura, S. Sakamoto, Y. Maeda, Y. Naruta
    • Journal Title

      Angewandte Chemie International Edition 48

      Pages: 9262-9267

    • Related Report
      2009 Final Research Report
  • [Journal Article] Influence of Membrane Curvature on the Structure of the Membrane-Associated Pleckstrin Homology Domain of Phospholipase C-δ12009

    • Author(s)
      N. Uekama, T. Aoki, T. Maruoka, S. Kurisu, A. Hatakeyama, S. Yamaguchi, M. Okada, H. Yagisawa, K. Nishimura, S. Tuzi
    • Journal Title

      Biochimica et Biophysica Acta - Biomembrane 1788

      Pages: 2575-2583

    • Related Report
      2009 Final Research Report
  • [Journal Article] Red-excitation resonance Raman analysis of the nu(Fe=0) mode of ferryl-oxo hemoproteins2009

    • Author(s)
      Ikemura K, et.al.
    • Journal Title

      J Am Chem Soc 130

      Pages: 14384-14385

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamics Change of Phoborhodopsin and Transducer by Activation: Study Using D75N Mutant of the Receptor by Site-directed Solid-state 13C NMR2008

    • Author(s)
      Kawamura, H. Yoshida, Y. Ikeda, S. Yamaguchi, S. Tuzi, H. Saito, N. Kamo, A. Naito.
    • Journal Title

      Photochem Photobiol. 84

      Pages: 291-230

    • Related Report
      2009 Final Research Report
  • [Journal Article] Red-Excitation Resonance Raman Analysis of the nFe=O Mode of Ferryl-Oxo Hemoproteins2008

    • Author(s)
      K. Ikemura, M. Mukai, H. Shimada, T. Tsukihara, S. Yamaguchi, K. Shinzawa-Itoh, S. Yoshikawa, T. Ogura
    • Journal Title

      J. Am. Chem. Soc. 130

      Pages: 14384-14385

    • Related Report
      2009 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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