Development of a Time-Resolved Infrared Spectrophotometer ～ Elucidation of the proton pumping mechanism ～

• Project/Area Number: 20770125
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: University of Hyogo
• Principal Investigator: YAMAGUCHI Satoru University of Hyogo, 大学院・生命理学研究科, 助教 (20347529)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 時間分解測定 / 赤外分光 / プロトンポンプ / 赤外分光法 / マイクロチップ / 生体エネルギー変換 / 構造生物学 / 膜蛋白質 / エネルギー変換

Research Abstract

A femtosecond infrared laser was combined to our infrared spectrometer for light source. A chamber was constructed to cover while optical system because the water vapor makes IR spectra worth remarkably and it was filled with dry N_2 gas. For time-resolved IR experiment, a micro flow cell that dimension has 50 x 10^<-6> m (light path length) and 400 x 10^<-6> m (flow pass width) was made by sandwiched SU-8 polymer between CaF_2 windows. A difference spectra between reduced state and oxidized state of cytochrome c oxidase was acquired by using these system.

Research Products

• [Journal Article] Participation of the BC loop in the correct folding of bacteriorhodopsin as revealed by solid-state NMR (2009)
  • Author(s): I. Kawamura, J. Tanabe, M. Ohmine, S. Yamaguchi, S. Tuzi, A. Naito
  • Journal Title: Photochemistry and Photobiology 85 Pages: 624-630
• [Journal Article] Spectroscopic Characterization of a Hydroperoxo-Heme Intermediate: Conversion of a Side-On Peroxo to an End-On Hydroperoxo (2009)
  • Author(s): J.G. Liu, T. Ohta, S. Yamaguchi, T. Ogura, S. Sakamoto, Y. Maeda, Y. Naruta
  • Journal Title: Angewandte Chemie International Edition 48 Pages: 9262-9267
• [Journal Article] Influence of Membrane Curvature on the Structure of the Membrane-Associated Pleckstrin Homology Domain of Phospholipase C-δ1 (2009)
  • Author(s): N. Uekama, T. Aoki, T. Maruoka, S. Kurisu, A. Hatakeyama, S. Yamaguchi, M. Okada, H. Yagisawa, K. Nishimura, S. Tuzi
  • Journal Title: Biochimica et Biophysica Acta - Biomembrane 1788 Pages: 2575-2583
• [Journal Article] Red-excitation resonance Raman analysis of the nu(Fe=0) mode of ferryl-oxo hemoproteins (2009)
  • Author(s): Ikemura K, et.al.
  • Journal Title: J Am Chem Soc 130 Pages: 14384-14385
  • Peer Reviewed
• [Journal Article] Dynamics Change of Phoborhodopsin and Transducer by Activation: Study Using D75N Mutant of the Receptor by Site-directed Solid-state 13C NMR (2008)
  • Author(s): Kawamura, H. Yoshida, Y. Ikeda, S. Yamaguchi, S. Tuzi, H. Saito, N. Kamo, A. Naito.
  • Journal Title: Photochem Photobiol. 84 Pages: 291-230
• [Journal Article] Red-Excitation Resonance Raman Analysis of the nFe=O Mode of Ferryl-Oxo Hemoproteins (2008)
  • Author(s): K. Ikemura, M. Mukai, H. Shimada, T. Tsukihara, S. Yamaguchi, K. Shinzawa-Itoh, S. Yoshikawa, T. Ogura
  • Journal Title: J. Am. Chem. Soc. 130 Pages: 14384-14385