Angle Dependence of the Affinity for Nucleotides in Rotary Motor F1-ATPase

• Project/Area Number: 20770127
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Waseda University
• Principal Investigator: ADACHI Kengo Waseda University, 理学部, 研究員 (60370128)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 1分子計測 / 1分子操作 / 分子モーター / エネルギー変換 / F1-ATPase / 蛍光性ヌクレオチドアナログ / アフィニティ / ヌクレオチド / 1分子計測(SMD) / 生物物理

Research Abstract

We measured the angle dependence of nucleotides affinity in the rotary molecular motor of F_1-ATPase by single-molecule manipulation with magnetic tweezers and simultaneously single-fluorophore imaging of fluorescent nucleotide analog. The binding/release angle of fluorescent ATP (Cy3-ATP) and fluorescent ADP (Cy3-ADP) were observed in the presence of unlabeled ATP, unlabeled ADP, and/or Pi. These results are expected to reveal the mechanism of hydrolysis rotation and ATP synthesis.

Research Products

• [Journal Article] Chemo-mechanical coupling in F_1-ATPase revealed by catalytic site occupancy during active catalysis. (2010)
  • Author(s): Rieko Shimo-Kon, Eiro Muneyuki, Hiroshi Sakai, Kengo Adachi, Masasuke Yoshida, Kazuhiko Kinosita Jr
  • Journal Title: Biophys. J. 98 Pages: 1227-1236
  • Peer Reviewed
• [Journal Article] Stimulation of F_1-ATPase activity by sodium dodecyl sulfate. (2010)
  • Author(s): Mohammad Delawar Hossain, Shou Furuike, Yasuhiro Onoue, Kengo Adachi, Masasuke Yoshida, Kazuhiko Kinosita Jr
  • Journal Title: Biochim. Biophys. Acta. (Bioenergetics) 1797 Pages: 435-442
  • Peer Reviewed
• [Journal Article] Stimulation of F_1-ATPase activity by sodium dodecyl sulfate. (2010)
  • Author(s): Mohammad Delawar Hossain, et al.
  • Journal Title: Biochimica et Biophysica Acta 1797 Pages: 435-442
  • Peer Reviewed
• [Journal Article] Chemo-Mechanical Coupling in F_1-ATPase Revealed by Catalytic Site Occupancy during Catalysis. (2010)
  • Author(s): Rieko Shimo-Kon, et al.
  • Journal Title: Biophysical Journal 98 Pages: 1227-1236
  • Peer Reviewed
• [Journal Article] Effect of epsilon subunit on the rotation of thermophilic Bacillus F_1-ATPase (2009)
  • Author(s): Masato Tsumuraya, Shou Furuike, Kengo Adachi, Kazuhiko Kinosita, Jr., Masasuke Yoshida
  • Journal Title: FEBS Lett. 583 Pages: 1121-1126
  • Peer Reviewed
• [Journal Article] Effect of subunit on the rotation of thermophilic Bacillus F1-ATPase. (2009)
  • Author(s): Masato Tsumuraya, et al.
  • Journal Title: FEBS Letters 583 Pages: 1121-1126
  • Peer Reviewed
• [Journal Article] Neither helix in the coiled coil region of the axle of F_1-ATPase plays a significant role in torque production (2008)
  • Author(s): Mohammad Delawar Hossain, Shou Furuike, Yasushi Maki, Kengo Adachi, Toshiharu Suzuki, Ayako Kohori, Hiroyasu Itoh, Masasuke Yoshida, Kazuhiko Kinosita, Jr
  • Journal Title: Biophys. J. 95 Pages: 4837-4844
  • Peer Reviewed
• [Journal Article] Temperature dependence of the rotation and hydrolysis activities of F_1-ATPase (2008)
  • Author(s): Shou Furuike, Kengo Adachi, Naoyoshi Sakaki, Rieko Shimo-Kon, Hiroyasu Itoh, Eiro Muneyuki, Masasuke Yoshida, Kazuhiko Kinosita, Jr
  • Journal Title: Biophys. J. 95 Pages: 761-770
  • Peer Reviewed
• [Journal Article] Axle-less F_1-ATPase rotates in the correct direction (2008)
  • Author(s): Shou Furuike, Mohammad Delawar Hossain, Yasushi Maki, Kengo Adachi, Toshiharu Suzuki, Ayako Kohori, Hiroyasu Itoh, Masasuke Yoshida, Kazuhiko Kinosita, Jr
  • Journal Title: Science 319 Pages: 955-958
  • Peer Reviewed
• [Journal Article] Axle-less F1-ATPase rotates in the correct direction (2008)
  • Author(s): Shou Furuike, et.al.
  • Journal Title: Science 319 Pages: 955958-955958
  • Peer Reviewed
• [Journal Article] Temperature dependence of the rotation and hydrolysis activities of F1-ATPase (2008)
  • Author(s): Shou Furuike, et.al.
  • Journal Title: Biophys. J 95 Pages: 761770-761770
  • Peer Reviewed
• [Journal Article] Neither helix in the coiled coil region of the axle of F1-ATPase plays a significant role in torque pr oduction (2008)
  • Author(s): Mohammad Delawar Hossein, et.al.
  • Journal Title: Biophys. J 95 Pages: 48374844-48374844
  • Peer Reviewed
• [Presentation] 回転分子モーターF_1-ATPaseの触媒部位へ結合したヌクレオチドの高精度位置検出(High-Accuracy Localization of Single Nucleotides Bound to the Catalytic Sites in Rotary Motor F_1-ATPase) (2009)
  • Author(s): 足立健吾、政池知子、大岩和宏、西坂崇之
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島文理大学・アスティとくしま
• [Presentation] 回転分子モーターF_1-ATPaseの触媒部位へ結合したヌクレオチドの高精度位置検出 (2009)
  • Author(s): 足立健吾、政池知子、大岩和宏、西坂崇之
  • Organizer: 日本生物物理学会第4回年会
  • Place of Presentation: 徳島文理大学・アスティとくしま
• [Presentation] F_1-ATPase回転モーターの角度依存的ヌクレオチドアフィニティ(Angle Dependence of the Affinity for Nucleotides in Rotary Motor F_1-ATPase) (2008)
  • Author(s): 足立健吾、大岩和宏、西坂崇之、野地博行、伊藤博康、吉田賢右、木下一彦
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] F1-APase回転モーターの角度依存的ヌクレオチドアフィニティ (2008)
  • Author(s): 足立健吾
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
• [Book] Physics and Biology(Graslund A, Rigler R and JerkerWidengren (eds. ), Chemo-Mechanical Coupling in the Rotary Molecular Motor F_1-ATPase., Vol. 96) (2010)
  • Author(s): Kengo Adachi, Shou Furuike, Mohammad Delawar Hossain, Hiroyasu Itoh, Kazuhiko Kinosita, Jr., Yasuhiro Onoue, Rieko Shimo-Kon
  • Publisher: Springer
• [Book] Chemo-Mechanical Coupling in the Rotary Molecular Motor F_1-ATPase. (2009)
  • Author(s): Kengo Adachi, et al.
  • Publisher: Single Molecule Spectroscopy in Chemistry, Physics and Biology Vol.96 (Springer)
• [Remarks]
  • URL: http://www.k2.phys.waseda.ac.jp/