Single-molecule imaging of DNA―protein interactions in DNA repair
| Project/Area Number |
20770131
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
YOKOTA Hiroaki Kyoto University, 物質-細胞統合システム拠点, 講師 (90415547)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 1分子計測(SMD) / 超精密計測 / ナノバイオ / 分子認識 / 核酸 |
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| Research Abstract |
To elucidate fundamental processes of DNA-protein interactions in DNA repair, we have performed single-molecule imaging of DNA-protein and protein-protein interactions using fluorescently labeled proteins involved in nucleotide excision repair and mismatch repair in Escherichia coli. The single-molecule observation in this study reveals DNA binding modes of a helicase and the number of the helicase bound to DNA.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Single-molecule visualization of binding modes of helicase to DNA on PEGylated surfaces.2009
Author(s)
Yokota, H., Han Y.W., Allem, J. -F., Xi, X.G., Bensimon, D., Croquette, V., Ito, Y., Harada, Y.
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Journal Title
Chem. Lett. 38
Pages: 308-309
NAID
Related Report
Peer Reviewed
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