Single-molecule imaging of DNA―protein interactions in DNA repair

• Project/Area Number: 20770131
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Kyoto University
• Principal Investigator: YOKOTA Hiroaki Kyoto University, 物質-細胞統合システム拠点, 講師 (90415547)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 1分子計測(SMD) / 超精密計測 / ナノバイオ / 分子認識 / 核酸

Research Abstract

To elucidate fundamental processes of DNA-protein interactions in DNA repair, we have performed single-molecule imaging of DNA-protein and protein-protein interactions using fluorescently labeled proteins involved in nucleotide excision repair and mismatch repair in Escherichia coli. The single-molecule observation in this study reveals DNA binding modes of a helicase and the number of the helicase bound to DNA.

Research Products

• [Journal Article] Single-molecule visualization of binding modes of helicase to DNA on PEGylated surfaces. (2009)
  • Author(s): Yokota, H., Han Y.W., Allem, J. -F., Xi, X.G., Bensimon, D., Croquette, V., Ito, Y., Harada, Y.
  • Journal Title: Chem. Lett. 38 Pages: 308-309
  • NAID: 10025120568
  • Peer Reviewed
• [Journal Article] Single-molecule Visualization of Binding Modes of Helicase to DNA on PEGylated Surfaces (2009)
  • Author(s): Hiroaki Yokota
  • Journal Title: Chemistry Letters 38 Pages: 308-309
  • NAID: 10025120568
  • Peer Reviewed
• [Presentation] Single-Molecule Visualization of the Oligomeric form of Escherichia coli UvrD helicase in vitro (2010)
  • Author(s): Yokota H.
  • Organizer: International Symposium on Hydration and ATP Energy
  • Place of Presentation: Hotel Iwanumaya, Akiu, Sendai, Japan
  • Year and Date: 2010-03-09
• [Presentation] Single-Molecule Visualization of the Oligomeric Form of Escherichia coli UvrD Helicase In Vitro (2010)
  • Author(s): Hiroaki Yokota
  • Organizer: International Symposium on Hydration and ATP Energy
  • Place of Presentation: Hotel Iwanumaya(仙台市)
  • Year and Date: 2010-03-09
• [Presentation] Single-molecule visualization of the oligomeric form of Escherichia coli UvrD helicase in vitro (2010)
  • Author(s): Yokota H.
  • Organizer: Biophysical Society 54th Annual Meeting
  • Place of Presentation: Moscone Center (サンフランシスコ)
  • Year and Date: 2010-02-22
• [Presentation] Single-Molecule Visualization of the Oligomeric Form of Escherichia coli UvrD Helicase In Vitro (2010)
  • Author(s): Hiroaki Yokota
  • Organizer: Biophysical Society 54th Annual Meeting
  • Place of Presentation: Moscone Center(サンフランシスコ)
  • Year and Date: 2010-02-22
• [Presentation] Single-molecule observation of DNA/helicase interactions. (2010)
  • Author(s): Yokota, H.
  • Organizer: The 13th Membrane Research Forum & The 6th iCeMS International Symposium Featuring Nan-Meso Membrane Mechanisms
  • Place of Presentation: ホテルフジタ京都(京都市)
  • Year and Date: 2010-01-28
• [Presentation] Single-molecule observation of DNA/helicase interactions (2010)
  • Author(s): Hiroaki Yokota
  • Organizer: The 13th Membrane Research Forum & The 6th iCeMS International Symposium Featuring Nan-Meso Membrane Mechanisms
  • Place of Presentation: ホテルフジタ京都
  • Year and Date: 2010-01-28
• [Presentation] Single-molecule observation of DNA-helicase interactions. (2009)
  • Author(s): Yokota, H.
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: アスティ徳島(徳島市)
  • Year and Date: 2009-11-01
• [Presentation] Single-molecule observation of DNA-helicase interactions (2009)
  • Author(s): Hiroaki Yokota
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: アスティ徳島
  • Year and Date: 2009-11-01
• [Presentation] Single-molecule imaging of DNA-protein interactions (2009)
  • Author(s): Yokota, H.
  • Organizer: 第5回阪大ナノサイエンス・ナノテクノロジー国際シンポジウム
  • Place of Presentation: 大阪大学銀杏会館(吹田市)
  • Year and Date: 2009-09-02
• [Presentation] Single-molecule imaging of DNA-protein interactions (2009)
  • Author(s): Hiroaki Yokota
  • Organizer: 第5回阪大ナノサイエンス・ナノテクノロジー国際シンポジウム
  • Place of Presentation: 大阪大学
  • Year and Date: 2009-09-02
• [Presentation] マイクロビーズとマイクロ流路を組み合わせた新規プロテインアレイの開発」科研費・特定領域研究・「マルチスケール操作によるシステム細胞工学(バイオ操作)」 (2009)
  • Author(s): 横田浩章
  • Organizer: 第7回公開シンポジウム
  • Place of Presentation: 東京エレクトロンホール宮城
  • Year and Date: 2009-03-06
• [Presentation] マイクロビーズとマイクロ流路を組み合わせた新規プロテインアレイの開発 (2009)
  • Author(s): 横田浩章
  • Organizer: 科研費・特定領域研究・「マルチスケール操作によるシステム細胞工学(バイオ操作)」第7回公開シンポジウム
  • Place of Presentation: 東京エレクトロンホール宮城
  • Year and Date: 2009-03-06
• [Presentation] DNAヘリカーゼUvrDの機能単位の1分子解析 (2009)
  • Author(s): 横田浩章
  • Organizer: 2009年生体運動研究合同班会議
  • Place of Presentation: 東京大学・数理科学研究科棟・大講義室(目黒区)
  • Year and Date: 2009-01-11
• [Presentation] DNAヘリカーゼUvrDの機能単位の1分子解析 (2009)
  • Author(s): 横田浩章
  • Organizer: 2009年生体運動研究合同班会議
  • Place of Presentation: 東京大学・数理科学研究科棟
  • Year and Date: 2009-01-11
• [Presentation] 同時計測顕微鏡によるDNA-ヘリカーゼ相互作用の1分子観察 (2008)
  • Author(s): Yokota, H.
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2008-12-04
• [Presentation] 同時計測顕微鏡によるDNA-ヘリカーゼ相互作用の1分子観察 (2008)
  • Author(s): 横田浩章
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-04
• [Presentation] Single-molecule observation of DNA/helicase interaction. (2008)
  • Author(s): Yokota, H.
  • Organizer: The GRC on Single Molecule Approaches to Biology Colby-Sawyer College
  • Place of Presentation: NH, USA
  • Year and Date: 2008-08-21
• [Presentation] Single-molecule observation of DNA/helicase interaction (2008)
  • Author(s): Hiroaki Yokota
  • Organizer: The GRC on Single Molecule Approaches to Biology
  • Place of Presentation: Colby-Sawyer College (NH, USA)
  • Year and Date: 2008-08-21
• [Patent(Industrial Property Rights)] 蛍光顕微鏡装置 (2010)
  • Inventor(s): 白川昌宏、原田慶恵、横田浩章、吉成洋祐、辻成悟
  • Industrial Property Rights Holder: 国立大学法人京都大学、日本電子株式会社
  • Industrial Property Number: 2010-021619
  • Filing Date: 2010-02-02
• [Patent(Industrial Property Rights)] 蛍光顕微鏡装置 (2010)
  • Inventor(s): 白川昌宏, 原田慶恵, 横田浩章, 吉成洋祐, 辻成悟
  • Industrial Property Rights Holder: 国立大学法人京都大学、日本電子株式会社
  • Industrial Property Number: 2010-021619
  • Filing Date: 2010-02-02