| Project/Area Number |
20770134
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HORIUCHI Keiko The University of Tokyo, 先端科学技術研究センター, 特任助教 (00456203)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 発現制御 / プロテオーム / マイクロアレイ / プロテオミクス解析 / 分子間相互作用 / 細胞周期 |
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| Research Abstract |
Wilms' tumor 1-associating protein (WTAP) has been reported to be a ubiquitously expressed nuclear protein and supposed to have a role in pre-mRNA splicing. We have reported that WTAP is involved in the stability of cyclin A2 mRNA thereby regulates the cell cycle and WTAP knockout mice exhibited proliferative failure with death at early embryonic stage. In this study, we have developed a method for the functional analysis of protein-protein interactions for the endogenous proteins, by affinity purification mass spectrometry using magnetic beads and high-affinity antibodies. Using the method, we identified several binding proteins of WTAP, including vrilizer homolog, E3 ubiquitin ligase, SR proteins and RNA bindin proteins. Furthermore we found the compositional changes of WTAP protein complex in cell cycle, indicating a relation to cell cle regulation of WTAP protein complex.
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