Regulations of mismatch repair proteins for cancer prevention

• Project/Area Number: 20770136
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: YOSHIOKA Ken-ichi Tokyo Medical and Dental University, 研究所, 主任研究官 (70321916)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ミスマッチ修復 / ゲノム不安定性 / マイクロサテライト不安定性 / DNA複製 / クロモソーマル不安定性 / DNA複製ストレス / NDA損傷 / 発がん / ミスマッチ修復因子 / 加齢 / H2AX

Research Abstract

There are two types of cancer associated genomic instabilities, i.e., either microsatellite or chromosomal instabilities, which develop dependent on either proficient or deficient in mismatch repair (MMR) function. Here we found that chromosomal instability is triggered by DNA replication stress-associated lesions in the presence of MMR and that senescence associated risk elevation of both genomic instabilities is ascribed to H2AX diminution during senescing process.

Research Products

• [Journal Article] DNA Lesions Induced by Replication Stress Trigger Mitotic Aberration and Tetraploidy Development. (2010)
  • Author(s): Ichijima, Y., Yoshioka, K., * Yoshioka, Y., Shinohe, K., Fujimori, H., Unno, J., Takagi, M., Goto, H., Inagaki, I., Mizutani, S., Teraoka, H.
  • Journal Title: PLoS ONE 5
• [Journal Article] DNA Lesions Induced by Replication Stress Trigger Mitotic Aberration and Tetraploidy Development. (2010)
  • Author(s): Yosuke Ichijima
  • Journal Title: PLoS ONE 5
  • Peer Reviewed
• [Journal Article] The Mismatch Repair-Mediated Cell Cycle Checkpoint Response to Fluorodeoxyuridine. (2008)
  • Author(s): Liu, A., Yoshioka, K., Salerno, V., Hsieh, P.
  • Journal Title: J Cellular Biochem 105 Pages: 245-254
• [Journal Article] The Mismatch Repair-Mediated Cell Cycle Checkpoint Response to Fluorodeoxyuridine (2008)
  • Author(s): Liu, A., Yoshioka, K., Salerno, V., & Hsieh, P.
  • Journal Title: J. Cellular Biochem 105 Pages: 245-254
  • Peer Reviewed
• [Presentation] 老化細胞の形質転換は、H2AXの減少-DNA修復能の低下-DNA損傷の蓄積-DNAの傷のM期への進行-ゲノム不安定化を経由して誘導される (2008)
  • Author(s): 吉岡研一
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 老化細胞の形質転換は、H2AXの減少-DNA修復能の低下-DNA損傷の蓄積DNAの傷のM期への進行-ゲノム不安定化を経由して誘導される (2008)
  • Author(s): 吉岡研一
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Remarks]
  • URL: http://www.ncc.go.jp/jp/nccri/divisions/02bioc/02bioc05.html
• [Remarks]
  • URL: http://www.ncc.go.jp/jp/nccri/divisions/02bioc/02bioc05.html