| Project/Area Number |
20770139
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
KEYAMURA Kenji Kyushu University, 薬学研究院, 助教 (70464386)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Keywords | DNA複製 / 複製開始複合体 / タンパク質相互作用 / 染色体DNA複製 / 開始複合体 / 大腸菌 / 染色体DNA複製開始 |
|---|
| Research Abstract |
In many cellular organisms, chromosomal replication initiation requires the regulated formation of dynamic higher-order complexes. E. coli DnaA-DiaA complexes form a specific multimer on oriC, resulting in DNA unwinding and DnaB helicase loading. DiaA, a DnaA-binding protein, directly stimulates the formation of ATP-DnaA multimers on oriC and ensures timely replication initiation. In this study, the structural model of DnaA-DiaA complex was determined. Moreover, a DiaA-regulating factor was searched.
|
