| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
(1) We have constracted baculovirus expression system of CRL4^<Cdt2> ubiqutin ligase, and in vitro ubiquitylation system for Cdt1, p21.(2) We have demonstrated that two conserved amino acid residues within and downstream of the PIP-box are required for degradation in mammalian cells. TD amino acid residues in the PIP-box, and the presence of basic amino acids at +3 and +4 downstream of the PIP box create a degron for CRL4^<Cdt2>. (3) We have analyzed the proteins which interacts with CRL4^<Cdt2> in vivo by mass spectrometry, and found candidates for ubiquitylation by CRL4^<Cdt2>.
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