Analysis of chromosomal translocation in humans by using medaka germ cell and its application

• Project/Area Number: 20770141
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Fujita Health University
• Principal Investigator: INAGAKI Hidehito Fujita Health University, 総合医科学研究所, 助教 (70308849)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 染色体転座 / DNAの高次構造 / DNA二重鎖切断 / DNA高次構造 / ゲノム / 核酸 / 発生・分化 / 動物 / 染色体

Research Abstract

Chromosomal translocations often cause a variety of diseases, such as cancers or infertility. To find mechanism of a frequently occurring translocation in humans as an example, I used medaka germ cells as a model. In addition, I made a translocation model system of a human cell line and made an attempt to find factors involving the translocation. As a result, I proposed a novel mechanism of the recurrent translocation. This result might lead to a new gene transfer technique in the future.

Research Products

• [Journal Article] Paternal origin of the de novo constitutional t(11;22)(q23;q11) (2010)
  • Author(s): Ohye T, Inagaki H, Kogo H, Tsutsumi M, Kato T, Tong M, Macville MV, Medne L, Zackai EH, Emanuel BS, Kurahashi H
  • Journal Title: Eur J Hum Genet (in press)
  • Peer Reviewed
• [Journal Article] Paternal origin of the de novo constitutional t(11;22)(q23;q11) (2010)
  • Author(s): Ohye, T.et al.
  • Journal Title: Eur.J.Hum.Genet. (In press)
  • Peer Reviewed
• [Journal Article] Impaired DNA replication prompts deletions within palindromic sequences, but does not induce translocations in human cells (2009)
  • Author(s): Kurahashi H, Inagaki H, Kato T, Hosoba E, Kogo H, Ohye T, Tsutsumi M, Bolor H, Tong M, Emanuel BS
  • Journal Title: Hum Mol Genet Vol.18、No.18 Pages: 3397-3406
  • Peer Reviewed
• [Journal Article] Recent advance in our understanding of the molecular nature of chromosomal abnormalities (2009)
  • Author(s): Kurahashi H, Bolor H, Kato T, Kogo H, Tsutsumi M, Inagaki H, Ohye T
  • Journal Title: J Hum Genet Vol.54、No.5 Pages: 253-260
  • NAID: 10030730167
• [Journal Article] Choromosomal instability mediated by non-B DNA: cruciform conformation and not DNA sequence is responsible for recurrent translocation in humans (2009)
  • Author(s): Inagaki H, Ohye T, Kogo H, Kato T, Bolor H, Taniguchi M, Shaikh TH, Emanuel BS, Kurahashi H
  • Journal Title: Genome Res Vol.19、No.2 Pages: 191-198
  • Peer Reviewed
• [Journal Article] Impaired DNA replication prompts deletions within palindromic sequences, but does not induce translocations in human cells (2009)
  • Author(s): Kurahashi, H.et al.
  • Journal Title: Hum.Mol.Genet. 18 Pages: 3397-3406
  • Peer Reviewed
• [Journal Article] Chromosomal instability mediated by non- B DNA : cruciform conformation and DNA sequence is responsible for recurrent translocation in humans (2009)
  • Author(s): Inagaki, H., et.al.
  • Journal Title: Genome Research 19 Pages: 191-198
  • Peer Reviewed
• [Journal Article] Two different forms of palindrome resolution in the human genome: deletion or translocation (2008)
  • Author(s): Kato T, Inagaki H, Kogo H, Ohye T, Yamada T, Emanuel BS, Kurahashi H
  • Journal Title: Hum Mol Genet Vol.17、no.8 Pages: 1184-1191
  • Peer Reviewed
• [Journal Article] Two different forms of palindrome resolution in the human genome : deletion or translocation (2008)
  • Author(s): Kato, T., et.al.
  • Journal Title: Human Molecular Genetics 17 Pages: 1184-1191
  • Peer Reviewed
• [Presentation] GEN1 resolves cruciformforming palindromic DNA leading to recurrent translocation in human (2009)
  • Author(s): 稲垣秀人
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-12
• [Presentation] GEN1 resolves cruciform-forming palindromic DNA leading to recurrent translocation in human (2009)
  • Author(s): 稲垣, 他
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-12-12
• [Presentation] Non-B型DNAが引き起こすヒト染色体転座の発生機構 (2009)
  • Author(s): 稲垣秀人
  • Organizer: 日本分子生物学会第9回春季シンポジウム
  • Place of Presentation: 宮崎市
  • Year and Date: 2009-05-11
• [Presentation] Artemisは十字架型のパリンドローム構造を切断し染色体転座を誘発する (2008)
  • Author(s): 稲垣秀人
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-11
• [Presentation] Artemisは十字架型のパリンドローム構造を切断し染色体転座を誘発する (2008)
  • Author(s): 稲垣秀人, 他
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2008-12-11
• [Presentation] Artemis cleaves cruciform- forming palindromic DNA leading to recurrent translocation in humans (2008)
  • Author(s): Inagaki H.
  • Organizer: 58th Meeting of The American Society of Human Genetics
  • Place of Presentation: Philadelphia
  • Year and Date: 2008-11-13
• [Presentation] Artemis cleaves cruciform- forming palindromic DNA leading to recurrent translocation in humans (2008)
  • Author(s): Inagaki, H., et.al.
  • Organizer: 58^<th> Annual meeting of The American Sciety of Human Genetics
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2008-11-13
• [Presentation] Artemis cleaves cruciform- forming palindromic DNA leading to recurrent translocation in humans (2008)
  • Author(s): Inagaki H.
  • Organizer: ASHG Ataxia-Telangiectasia/Genome Instability Satellite Meeting
  • Place of Presentation: Philadelphia
  • Year and Date: 2008-11-11