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Analysis of proteins regulating bacterial cell division site

Research Project

Project/Area Number 20770142
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionUniversity of Toyama

Principal Investigator

OKUNO Takashi  University of Toyama, 大学院・医学薬学研究部(薬学), 准教授 (80411031)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords細胞分裂 / ATPase / 分子間相互作用
Research Abstract

We have studied on the Min proteins (MinC,D,E) which regulates position of ceptum ring (FtsZ ring) formation in E.coli. he results of FRET analysis indicated that MinC interacts with FtsZ monomer rather than FtsZ polymer in the solution. It is suggested that interaction between monomeric FtsZ and MinC is important for controlling of FtsZ polymerization by MinC. On the other hand, in vitro analysis of mutant proteins of MinD indicated membrane binding of MinD requires not only ATP binding to nucleotide binding pocket of MinD, but also other factors.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (13 results)

All 2010 2009 2008 Other

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] Comparison of intracellular localization between Nubp1 and Nubp2 utilizing GFP fusion protein.2010

    • Author(s)
      Okuno T., Yamabayashi H., Kogure K.
    • Journal Title

      Mol. Biol. Rep. 37

      Pages: 1165-1168

    • Related Report
      2009 Final Research Report
  • [Journal Article] Comparison of intracellular localization between Nubpl and Nubp2 utilizing GFP fusion protein.2010

    • Author(s)
      Okuno T., Yamabayashi H., Kogure K.
    • Journal Title

      Mol.Biol.Rep. 37

      Pages: 1165-1168

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct monitoring of interaction between Escherichia coli proteins., MinC and monomeric FtsZ, in solution.2009

    • Author(s)
      Okuno T., Ogoh M., Tanina H., Funasaki N., Kogure K.
    • Journal Title

      Biol. Pharm. Bull. 32

      Pages: 1473-1475

    • NAID

      130000117267

    • Related Report
      2009 Final Research Report
  • [Journal Article] Direct monitoring of interaction between Escherichia coli proteins, MinC and monomeric FtsZ, in solution.2009

    • Author(s)
      Okuno T., Ogoh M., Tanina H ., Funasaki N., Kogure K.
    • Journal Title

      Biol.Pharm.Bull. 32

      Pages: 1473-1475

    • NAID

      130000117267

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins.2009

    • Author(s)
      Okuno T., Yamabayashi H., Kogure K., Ueno M.
    • Organizer
      日本分子生物学会第32回年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Final Research Report
  • [Presentation] Comparison of intracellular localization of Nubp 1 and Nubp2 using GFP fusion proteins.2009

    • Author(s)
      Okuno T., Yamabayashi H., Kogure K., Ueno M.
    • Organizer
      日本分子生物学会第32回年会
    • Place of Presentation
      横浜市
    • Year and Date
      2009-12-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規の平面脂質膜構造体の膜タンパク質機能のイメージングへの応用2009

    • Author(s)
      奥野貴士, 小暮健太朗, 上野雅晴
    • Organizer
      日本薬学会北陸支部121回例会
    • Place of Presentation
      富山
    • Year and Date
      2009-12-05
    • Related Report
      2009 Final Research Report
  • [Presentation] 新規の平面脂質膜構造体の膜タンパク質機能のイメージングへの応用2009

    • Author(s)
      奥野貴士, 小暮健太朗, 上野雅晴
    • Organizer
      日本薬学会北陸支部12回例会
    • Place of Presentation
      富山市
    • Year and Date
      2009-12-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Analysis of interaction between MinC and FtsZ by FRET.2008

    • Author(s)
      奥野貴士, 丸山真由美, 小郷真智子, 谷名宏允, 舟崎紀昭, 小暮健太朗
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡
    • Year and Date
      2008-12-03
    • Related Report
      2009 Final Research Report
  • [Presentation] FRETを利用したバクテリア分裂制御タンパク質間の相互作用の解析2008

    • Author(s)
      奥野貴士, 小郷真智子, 谷名宏允, 舟崎紀昭, 小暮健太朗
    • Organizer
      第58回日本薬学会近畿支部大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-10-25
    • Related Report
      2009 Final Research Report
  • [Presentation] FRETを利用したバクテリア分裂制御タンパク質問の相互作用の解析2008

    • Author(s)
      奥野貴士, 小郷真智子, 谷名宏允, 舟崎紀昭, 小暮健太朗
    • Organizer
      第58回日本薬学会近畿支部大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-10-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] Analysis of interaction between MinC and FtsZ by FRET2008

    • Author(s)
      奥野貴士, 丸山真由美, 小郷真智子, 谷名宏允, 舟崎紀昭, 小暮健太朗
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.pha.u-toyama.ac.jp/phypha1/index-j.html

    • Related Report
      2009 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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