| Project/Area Number |
20770144
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
YAGURA Masaru National Institute of Genetics, 細胞遺伝研究系, 研究員 (00455205)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | DNA複製開始機構 / 染色体DNA / 出芽酵母 / 生化学 / 遺伝学 |
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| Research Abstract |
In eukaryotes, many initiation factors are assembled and disassembled on chromatin, and one of the factor, Mcm10, was thought to be involved in important role for the complex formation. I carefully assessed how Mcm10 participates in DNA replication. A thermosensitive allele of MCM10, mcm10-1 significantly reduced DNA replication at the nonpermissive condition after release from G1 block. Biochemical analysis revealed that budding yeast Mcm10 is a long and narrow shape and form a monomer, and it can bind to some DNA replication initiation factors directly.
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