Identification and characterization of transcripts transmitted from sperm to oocyte
| Project/Area Number |
20770145
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KAWANO Mitsuoki The Institute of Physical and Chemical Research, LSA要素技術開発ユニット, 研究員 (00455338)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 精子RNA / 生殖細胞特異的小分子RNA / 世代伝達性RNA / 核局在モチーフ配列 / 精子 / 小分子RNA / 機能性RNA / 生殖細胞 / 次世代シーケンサー / 核移行シグナル / エピジェネティクス / 世代伝達性物質 / 遺伝情報物質 / 受精 / エピジェネティックス / 進化 / 遺伝子発現制御 |
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| Research Abstract |
I report the discovery by deep sequencing technology of a suite of small noncoding RNAs in mature mouse sperm. Two of them were highly enriched in sperm and detectable exclusively in germ cells. Designated 'spR-12 and -13' for 'sperm RNAs'. After fertilization, they were maintained in low copy numbers in the egg and the dividing blastomeres. A strict nuclear localization was evidenced by microinjection of fluorescence-tagged RNAs into the male pronucleus of one-cell embryos. Stably maintained during the preimplantation period, they were equally distributed between blastomeres at cell division, remaining strictly localized in the nucleus until the blastocyst stage at which they were not detectable. A sequence motif UGGG(G)CGGG common to the two spRs was sufficient for nuclear addressing of unrelated small RNAs. A knock-down experiment performed by microinjection of the spR-12 and spR-13 complementary sequences linked to the nuclear addressing motif showed a deleterious effect suggestive of a functional role of the two RNAs in early embryogenesis.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] miRNA profiling of bilateral rat hippocampal CA3 by deep sequencing.2011
Author(s)
Shinohara, Y., Yahagi, K., Kawano, M., Nishiyori, H., Kawazu, C., Suzuki, N., Manabe, R., Hirase, H.
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Journal Title
Biochem. Biophys.Res.Commun. 409
Pages: 293-298
NAID
Related Report
Peer Reviewed
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[Journal Article] Reduction of non-insert sequence reads by "dimer eliminator" LNA oligonucleotide for small RNA deep sequencing.2010
Author(s)
Kawano, M., Kawazu, C., Lizio, M., Kawaji, H., Carninci, P., Suzuki, H., Hayashizaki, Y.
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Journal Title
Biotechniques. 49(4)
Pages: 751-755
Related Report
Peer Reviewed
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[Journal Article] Efficient small RNA library preparation protocol.2010
Author(s)
Kawano, M., Kawazu, C., Lizio, M., Kawaji, H., Carninci, P., Suzuki, H., Hayashizaki, Y.
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Journal Title
Biotechniques Protocol Guide 2011
Pages: 4-6
Related Report
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[Journal Article] Cross-mapping and the identification of editing sites in mature microRNAs in high-throughput sequencing libraries.2010
Author(s)
de Hoon, M. J., Taft, R.J., Hashimoto, T., Kanamori-Katayama, M., Kawaji, H., Kawano, M., Kishima, M., Lassmann, T., Faulkner, J., Mattick, J. S., Daub, C. O., Carninci, P., Kawai, J., Suzuki, H., Hayashizaki, Y.
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Journal Title
Genome Res. 20(2)
Pages: 257-264
Related Report
Peer Reviewed
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[Presentation] RNA in sperm : Towards an identification of RNAs transmitting genetic information to zygote.2008
Author(s)
Ma Kawano, M., Kawaji, H., Hasegawa, A., Severin, J., Sano, H., Hayashizaki, Y.
Organizer
Joint 7th Human Genome Organization (HUGO)-Pacific Meeting and the 8th Asia-Pacific Conference on Human Genetics, Symposium : Transmissible Nucleic Acids
Place of Presentation
Cebu, Philippines
Related Report
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[Presentation] RNA in sperm : Towards an identification of RNAs transmitted from sperm to zygote on fertilization.2008
Author(s)
Kawano, M., Hasegawa, A., Sano, H., Kawaji, H., Severin, J., Lassmann, T., Hayashizaki, Y.
Organizer
Miami Winter Symposium Regulatory RNA in Biology and Human Health,
Place of Presentation
Miami, USA
Related Report
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