Study of the function of Yip1A in Golgi-to-endoplasmic reticulum retrograde transport by using semi-intact cell assay

• Project/Area Number: 20770151
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: The University of Tokyo
• Principal Investigator: KANO Fumi The University of Tokyo, 大学院・総合文化研究科, 助教 (10361594)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 小胞輸送 / セミインタクト細胞 / ゴルジ体 / 小胞体 / 再構成 / Yip1A / 逆行輸送

Research Abstract

We studied the function of Yip1A in the intracellular vesicular transport by establishing the assay for the transports in permeabilized cell "semi-intact cell" system. We found that Yip1A regulated the retrograde transport from the Golgi apparatus to the endoplasmic reticulum (ER), and the results were published in the Journal of Cell Science. We also identified the Yip1A interacting proteins, which would be the possible regulatory components for the Golgi-to-ER transport.

Research Products

• [Journal Article] Hydrogen peroxide depletes phosphatidylinositol-3-phosphate from endosomes in a p38 MAPK-dependent manner and perturbs endocytosis. (2011)
  • Author(s): Kano F, Arai T, Matsuto M, Hayashi H, Sato M, Murata M.
  • Journal Title: Biochim Biophys Acta.-Mol. Cell Res. 1813 Pages: 784-801
• [Journal Article] Hydrogen peroxide depletes phosphatidylinositol-3-phosphate from endosomes in a p38 MAPK-dependent manner and perturbs endocytosis. (2011)
  • Author(s): Kano F, Arai T, Matsuto M, Hayashi H, Sato M, Murata M.
  • Journal Title: Biochim Biophys Acta.Mol.Cell Res. Volume: 1813 Pages: 784-801
  • Peer Reviewed
• [Journal Article] An orally applicable Shiga toxin neutralizer functions in the intestine to inhibit the intracellular transport of the toxin. (2010)
  • Author(s): Watanabe-Takahashi M, Sato T, Dohi T, Noguchi N, Kano F, Murata M, Hamabata T, Natori Y, Nishikawa K.
  • Journal Title: Infect Immun 78 Pages: 178-183
• [Journal Article] Golgi-associated GSK3beta regulates the sorting process of post-Golgi membrane trafficking. (2010)
  • Author(s): Adachi A, Knano F, Tsuboi T, Fujita M, Maeda Y, Murata M.
  • Journal Title: J. Cell Sci. 123 Pages: 3215-3225
• [Journal Article] An orally applicable Shiga toxin neutralizer functions in the intestine to inhibit the intracellular transport of the toxin. (2010)
  • Author(s): Watanabe-Takahashi M, Sato T, Dohi T, Noguchi N, Kano F, Murata M, Hamabata T, Natori Y, Nishikawa K.
  • Journal Title: Infect Immun Volume: 78 Pages: 178-183
  • Peer Reviewed
• [Journal Article] Golgi-associated GSK3beta regulates the sorting process of post-Golgi membrane trafficking. (2010)
  • Author(s): Adachi A, Kano F, Tsuboi T, Fujita M, Maeda Y, Murata M.
  • Journal Title: J Cell Sci. Volume: 123 Pages: 3215-25
  • Peer Reviewed
• [Journal Article] An orally applicable Shiga toxin neutralizer functions in the intestine to inhibit the intracellular transport of the toxin. (2010)
  • Author(s): Watanabe-Takahashi
  • Journal Title: Infection and Immunity 78 Pages: 17-183
  • Peer Reviewed
• [Journal Article] Visual screening and analysis for kinase-regulated membrane trafficking pathways that are involved in extensive beta-amyloid secretion. (2009)
  • Author(s): Adachi A, Kano F, Saido TC, Murata M
  • Journal Title: Genes to Cells 14 Pages: 355-369
• [Journal Article] YiplA regulates the retrograde transport form the Golgi apparatus to the endoplasmic reticulum. (2009)
  • Author(s): Kano. F., Yamauchi, S., Yoshida, Y., Watanabe-Takahashi, M., Nishikawa, K., Nakamura, N., Murata. M
  • Journal Title: Journal of Cell Science 122 Pages: 2218-2227
• [Journal Article] Visual screening and analysis for kinase-regulated membrane trafficking pathways that are involved in extensive beta-amyloid secretion. (2009)
  • Author(s): Adachi A, Kano F, Saido TC, Murata M
  • Journal Title: Genes to Cells Volume: 14 Pages: 355-369
  • Peer Reviewed
• [Journal Article] Yip1A regulates the retrograde transport form the Golgi apparatus to the endoplasmic reticulum. (2009)
  • Author(s): Kano. F., Yamauchi, S., Yoshida, Y., Watanabe-Takahashi, M., Nishikawa, K., Nakamura, N., Murata. M
  • Journal Title: Journal of Cell Science Volume: 122 Pages: 2218-2227
  • Peer Reviewed
• [Journal Article] Expression of the peroxisome proliferator activated receptor gamma gene is repressed by DNA methylation in visceral adipose tissue of mouse models of diabetes. (2009)
  • Author(s): Fujiki, K., Kano, F., Murata, M.
  • Journal Title: BMC Biology Volume: 17(online) Pages: 38-38
  • Peer Reviewed
• [Journal Article] Intracellular Phospholipase A1 iPLA1(gamma) is a Novel Factor Involved in COPI- and Rab6-Independent Retrograde Transport between the ER and the Golgi Complex. (2009)
  • Author(s): Morikawa, R.K., Aoki, J., Kano, F., Murata, M., Yamamoto, A., Tsujimoto, M., Arai, H.
  • Journal Title: Journal of Biological Chemistry Volume: 284 Pages: 26620-26630
  • Peer Reviewed
• [Journal Article] Yip1A regulates the COPI-independent retrograde transport from the Golgi complex to the ER (2009)
  • Author(s): Kano
  • Journal Title: Journal of Cell Science 122 Pages: 2218-2227
  • Peer Reviewed
• [Journal Article] Intracellular phospholipase Algamma (iPLAlgamma) is a novel factor involved in coat protein complex I- and Rab6-independent retrograde transport between the endoplasmic reticulum and the Golgi complex. (2009)
  • Author(s): Morikawa
  • Journal Title: Journal of Biological Chemistry 284 Pages: 26620-26630
  • Peer Reviewed
• [Journal Article] Visual screening and analysis for kinase-regulated membrane trafficking pathways that are involved in extensive beta-amyloid secretion. (2009)
  • Author(s): Adachi
  • Journal Title: Genes to Cells 14 Pages: 355-369
  • Peer Reviewed
• [Journal Article] Intracellular Phospholipase A1 iPLA1(gamma) is a Novel Factor Involved in COPI- and Rab6-Independent Retrograde Transport between the ER and the Golgi Complex.
  • Author(s): Morikawa, R.K., Aoki, J., Kano, F., Murata, M., Yamamoto, A., Tsujimoto, M., Arai, H.
  • Journal Title: Journal of Biological Chemistry 284 Pages: 26620-26630
• [Presentation] p38-dependent loss of phosphatidylinositol-3-phosphate at endosomes affects the retrograde transport of cholera toxin from cell surface to the Golgi BMB2010 (2010)
  • Author(s): F.Kano, T.Arai, M.Matsuto, M.Murata.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] p38-dependent loss of phosphatidylinositol-3-phosphate at endosomes affects the retrograde transport of cholera toxin from cell surface to the Golgi (2010)
  • Author(s): F.Kano, T.Arai, M.Matsuto, M.Murata
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Molecular mechanisms for the targeting of YiplA to ER-Golgi intermediate compartment BMB2010 (2010)
  • Author(s): 田口由起, 加納ふみ, 村田昌之
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Molecular mechanisms for the targeting of Yip1A to ER-Golgi intermediate compartment (2010)
  • Author(s): 田口由起, 加納ふみ, 村田昌之
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] セミインタクト細胞リシール法を用いたシグナル伝達経路解析システムの構築 (2010)
  • Author(s): 加納ふみ、荒井珠貴、松戸真理子、村田昌之
  • Organizer: 細胞を創る研究会3.0
  • Place of Presentation: 先端科学技術研究センター
  • Year and Date: 2010-11-12
• [Presentation] セミインタクト細胞を用いたRab6Aのゴルジ体へのターゲティングの再構成 (2010)
  • Author(s): 松戸真理子、加納ふみ、荒井珠貴、村田昌之
  • Organizer: 細胞を創る研究会3.0
  • Place of Presentation: 先端科学技術研究センター
  • Year and Date: 2010-11-12
• [Presentation] Murata Establishment of semi-intact cell system and application of its resealing technique : a study of membrane dynamics (2010)
  • Author(s): F.Kano, T.Arai, M.Matsuto, M.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2010-09-21
• [Presentation] Establishment of semi-intact cell system and application of its resealing technique : a study of membrane dynamics (2010)
  • Author(s): F.Kana, T.Arai, M.Matsuto, M.Murata
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2010-09-21
• [Presentation] セミインタクト細胞を用いたオルガネラダイナミクスの分子機構解明 (2010)
  • Author(s): 加納ふみ、村田昌之
  • Organizer: 日本薬学会第130年会(招待講演)
  • Place of Presentation: 岡山大学
  • Year and Date: 2010-03-29
• [Presentation] セミインタクト細胞を用いたオルガネラダイナミクスの分子機構解明 (2010)
  • Author(s): 加納ふみ、村田昌之
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山大学
  • Year and Date: 2010-03-29
• [Presentation] Mechanistic Insights into Menbrane Dynamics Using Semi-Intact Cell System (2009)
  • Author(s): 加納ふみ
  • Organizer: 第47回日本生物物理学会年会(招待講演)
  • Place of Presentation: 徳島文理大学
  • Year and Date: 2009-10-01
• [Presentation] Mechanistic Insights into Membrane Dynamics Using Semi-Intact Cell System (2009)
  • Author(s): 加納ふみ
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島文理大学
  • Year and Date: 2009-10-01
• [Presentation] 生命現象の可視化解析ツールとしてのセミインタクト細胞系 (2009)
  • Author(s): 加納ふみ
  • Organizer: 日本化学会第3回関東支部大会(招待講演)
  • Place of Presentation: 早稲田大学
  • Year and Date: 2009-09-01
• [Presentation] 生命現象の可視化解析ツールとしてのセミインタクト細胞系 (2009)
  • Author(s): 加納ふみ
  • Organizer: 日本化学会第3回関東支部大会
  • Place of Presentation: 早稲田大学
  • Year and Date: 2009-09-01
• [Remarks] ホームページ等
  • URL: http://muratalab.c.u-tokyo.ac.jp/