Functional characterization of novel proteins involved in mitochondrial dynamics

• Project/Area Number: 20770156
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: NAKAMURA Nobuhiro Tokyo Institute of Technology, 大学院・生命理工学研究科, 助教 (80361765)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 細胞構造・機能 / ミトコンドリア / ユビキチン / 精子形成 / 脱ユビキチン化酵素 / 変異体 / ゼブラフィッシュ / 酵素活性 / RNAi / 細胞生物学 / ミトコンドリアダイナミクス / 精巣

Research Abstract

This study has demonstrated that 1) an enzymatic activity of human USP30, a novel mitochondrial deubiquitinating enzyme, is required for maintenance of mitochondrial morphology, and 2) mouse GGNBP1 is highly expressed in spermatogenic cells and induces Drp1-dependent mitochondrial fragmentation. These results provide better understand of the molecular mechanism of mitochondrial dynamics.

Research Products

• [Journal Article] Rhesus glycoprotein p2 (Rhp2) is a novel member of the Rh family of ammonia transporters highly expressed in shark kidney. (2010)
  • Author(s): Nakada T, Westhoff CM, Yamaguchi Y, Hyodo S, Li X, Muro T, Kato A, Nakamura N, Hirose S
  • Journal Title: ournal of Biological Chemistry 285(4) Pages: 2653-2664
  • Peer Reviewed
• [Journal Article] Mechanism of development of ionocytes rich in vacuolar-type H+-ATPase in the skin of zebrafish larvae. (2009)
  • Author(s): Esaki M, Hoshijima K, Nakamura N, Munakata K, Tanaka M, Ookata K, Asakawa K, Kawakami K, Wang W, Weinberg ES, Hirose S
  • Journal Title: Developmental Biology 329(1) Pages: 116-129
  • Peer Reviewed
• [Journal Article] A novel potential role for gametogenetin-binding protein 1 (GGNBP1) in mitochondrial morphogenesis during spermatogenesis in mice. (2009)
  • Author(s): Aihara T, Nakamura N, Honda S, Hirose S
  • Journal Title: Biology of Reproduction 80(4) Pages: 762-770
  • Peer Reviewed
• [Journal Article] Mechanism of development of ionocytes rich in vacuolar-type H^+-ATPase in the skin of zebrafish larvae (2009)
  • Author(s): M Esaki, K Hoshijima, N Nakamura, K Munakata, 他6名, S Hirose
  • Journal Title: Developmental Biology 329(1) Pages: 116-129
  • Peer Reviewed
• [Journal Article] A novel potential role for gametogenetin-binding protein 1(GGNBP1)in mitochondrial morphogenesis during spermatogenesis of mice (2009)
  • Author(s): Aihara T., Nakamura N., Honda S., Hirose S.
  • Journal Title: Biology of Reproduction 80(4) Pages: 762-770
  • Peer Reviewed
• [Journal Article] Regulation of mitochondrial morphology by USP30 (2008)
  • Author(s): Nakamura N, Hirose S
  • Journal Title: a deubiquitinating enzyme present in the mitochondrial outer membrane. Molecular Biology of the Cell 19(5) Pages: 1903-1911
  • Peer Reviewed
• [Journal Article] Regulation of mitochondrial morphology by USP30, a deubiquitinatingenzyme present in the mitochondrial outer membrane (2008)
  • Author(s): Nakamura N., Hirose S.
  • Journal Title: Molecular Biology of the Cell 19(5) Pages: 1903-1911
  • Peer Reviewed
• [Presentation] Regulation of mitochondrial morphology by USP30, a novel mitochondrial deubiquitinating enzyme (2008)
  • Author(s): Nakamura N, Hirose S
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-29
• [Presentation] ミトコンドリア形態制御に関与する脱ユビキチン化酵素USP30の同定 (2008)
  • Author(s): 中村信大, 広瀬茂久
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-29
• [Remarks]
  • URL: http://www.hirose.bio.titech.ac.jp/index.html
• [Remarks]
  • URL: http://www.hirose.bio.titech.ac.jp/