Functional analysis of lysosomal protein trafficking in the fruit fly, Drosophila Melanogaster.

• Project/Area Number: 20770159
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Fukushima Medical University
• Principal Investigator: KAMETAKA Satoshi Fukushima Medical University, 医学部, 講師 (10303950)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: リソソーム / クラスリン / 小胞輸送 / エンドサイトーシス / ショウジョウバエ / ゴルジ体 / 選別輸送 / ショウジョウバェ / 試験管内再構成 / GGA

Research Abstract

Intracellular trafficking of integral membrane proteins requires formation of transport carriers to reach their final destination. Clathrin coat is a major coatmer responsible for generation of transport vesicles at the post-Golgi compartments. Clathrin adaptors such as GGA and AP-1 function at the Golgi membrane to recruit clathrin from the cytoplasm to the site of clathrin coated vesicle formation. This study was aimed to dissect the functions of GGA and AP-1 using fly cultured cells as a model system for mammals. We carried out the functional characterization of these clathrin adaptors and found that the molecular functions of the clathrin adaptors are highly conserved from fly to mammals.

Research Products

• [Journal Article] Functional characterization of protein-sorting machineries at the trans-Golgi network in Drosophila melanogaster. (2010)
  • Author(s): Satoshi Kametaka, Naoki Sawada, Juan S. Bonifacino, Satoshi Waguri
  • Journal Title: Journal of Cell Science 123 Pages: 460-470
  • Peer Reviewed
• [Journal Article] Functional characterization of protein sorting machineries at the TGN in the fruit fly, Drosophila melanogaster (2010)
  • Author(s): Satoshi Kametaka
  • Journal Title: Journal of Cell Science 123 Pages: 460-471
  • Peer Reviewed
• [Presentation] リソソーム酵素の選別輸送メカニズムの解析 (2010)
  • Author(s): 亀高諭、澤田直樹、池田寛子、和栗聡
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-28
• [Presentation] リソソーム酵素の選別輸送メカニズムの解析 (2010)
  • Author(s): 亀高諭
  • Organizer: 第115回 日本解剖学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-28
• [Presentation] ショウジョウバエ細胞を用いたクラスリンアダプターの機能解析 (2009)
  • Author(s): 亀高諭、Juan S. Bonifacino、和栗聡
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-04
• [Presentation] ショウジョウバエ細胞を用いたクラスリンアダフターの機能解析 (2009)
  • Author(s): 亀高諭
  • Organizer: 第61回 日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-04
• [Presentation] ショウジョウバエ細胞を用いたクラスリンアダプターの機能解析 (2009)
  • Author(s): 亀高諭、和栗聡
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-28
• [Presentation] ショウジョウバエ細胞を用いたクラスリンアダプターの機能解析 (2009)
  • Author(s): 亀高諭, 和栗聡
  • Organizer: 日本解剖学会第114回全国学術集会
  • Place of Presentation: 岡山理科大学
• [Presentation] HeLa細胞で見られたGGA1, 2, 3の特異的発現低下による表現型の違い (2008)
  • Author(s): 池田寛子、亀高諭、和栗聡
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-30
• [Book] ポストゴルジではたらくクラスリンアダプター、AP-1およびGGAの機能調節機構 (2008)
  • Author(s): 亀高諭、Juan S. Bonifacino
  • Publisher: 共立出版
• [Remarks]
  • URL: http://www.fmu.ac.jp/cms/anatomy2/index_html