Analysis of securin and separase functions in mammalian cell cycle regulation.

• Project/Area Number: 20770162
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Japanese Foundation For Cancer Research
• Principal Investigator: KUMADA Kazuki Japanese Foundation For Cancer Research, 細胞生物部, 研究員 (10370149)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 染色体分配 / DNA損傷応答 / 細胞周期 / ノックアウトマウス / 発がん / 糖尿病 / 癌

Research Abstract

I analyzed the detailed functions of separase and securin proteins in mammalian cell regulation, and found that both proteins cooperatively played another unknown role in DNA damage response, besides the previously well-known one in chromosome separation. I also found that the oncogenic activity of securin protein mainly resulted from the disturbance of its function in chromosome separation.

Research Products

• [Journal Article] Kinetochore stretching inactivates the spindle assembly checkpoint. (2009)
  • Author(s): Uchida KS, Takagaki K, Kumada K, Hirayama Y, Noda T, Hirota T.
  • Journal Title: Journal of Cell Biology. Vol.184 Pages: 383-90
  • Peer Reviewed
• [Journal Article] Kinetochore stretching inactivates the spindle assembly checkpoint. (2009)
  • Author(s): K. S. Uchida, K. Takagaki, K. Kumada, Y. Hirayama, T. Noda and T. Hirota
  • Journal Title: J. Cell Biol. 184 Pages: 383-390
  • Peer Reviewed
• [Presentation] 変異securinタンパク質発現による形質転換と細胞周期異常 (2010)
  • Author(s): 熊田和貴
  • Organizer: 第27回染色体ワークショップ
  • Place of Presentation: 御殿場高原ホテルB.U.
  • Year and Date: 2010-01-21
• [Presentation] 変異 securin タンパク質発現による形質転換と細胞周期異常 (2010)
  • Author(s): 熊田和貴
  • Organizer: 第27回染色体ワークショップ
  • Place of Presentation: 御殿場高原ホテルB.U.
  • Year and Date: 2010-01-21
• [Presentation] 細胞周期・DNA損傷応答・細胞形質転換に対する変異securinタンパク質発現の影響 (2009)
  • Author(s): 熊田和貴
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-01
• [Presentation] 細胞周期・DNA損傷応答・細胞形質転換に対する変異 securin タンパク質発現の影響 (2009)
  • Author(s): 熊田和貴
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-01
• [Presentation] separaseとsecurinのノックアウトマウスを用いた機能解析 (2009)
  • Author(s): 熊田和貴
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: ウェルサンピア姫路ゆめさき
  • Year and Date: 2009-01-27
• [Presentation] separaseとsecurinのノックアウトマウスを用いた機能解析 (2009)
  • Author(s): 熊田和貴、野田哲生
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 兵庫県姫路市・ウエルサンピア姫路ゆめさき
  • Year and Date: 2009-01-27
• [Presentation] 哺乳類セキュリンとセパラーゼはDNA損傷後の正常な細胞周期進行に協調して機能する (2008)
  • Author(s): 熊田和貴
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] 哺乳類セキュリンとセパラーゼはDNA損傷後の正常な細胞周期進行に強調して機能する (2008)
  • Author(s): 熊田和貴、野田哲生
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 愛知県名古屋市・名古屋国際会議場
  • Year and Date: 2008-10-28