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Spatio-temporal imaging of ER membrane proteinsp' dynamics and their interaction

Research Project

Project/Area Number 20770163
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

KUROKAWA Kazuo  The Institute of Physical and Chemical Research, 中野生体膜研究室, 専任研究員 (40333504)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords小胞体膜タンパク質 / ダイナミクス / 相互作用 / イメージング / 小胞体 / COPII
Research Abstract

In this study, we focused on the diffusion of and the interaction between Sar1p and Sec12p on the daughter and the mother ER in living yeast. There was no difference in their interaction and Sec12p diffusion between the daughter and the mother ER. However, Sar1p showed a slower diffusion on the daughter than on the mother. Furthermore, Sar1p diffusion rate depended on the size of daughter cell.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (11 results)

All 2010 2009 2008 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (7 results)

  • [Journal Article] Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3.2010

    • Author(s)
      Yamashita M, Kurokawa K, Sato Y, Yamagata A, Mimura H, Yoshikawa A, Sato K, Nakano A, Fukai S.
    • Journal Title

      Nat. Struct. & Mol. Biol. 17

      Pages: 180-186

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for the Rho-and phosphoinositide-dependent localization of the exocyst subunit Sec32010

    • Author(s)
      Yamashita, M.
    • Journal Title

      Nat.Struct.& Mol.Biol. 17

      Pages: 180-186

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human Mena associates with Rac1 small GTPase in glioblastoma cell lines.2009

    • Author(s)
      Higashi M, Ishikawa C, Yu J, Toyoda A, Kawana H, Kurokawa K, Matsuda M, Kitagawa M, Harigaya K.
    • Journal Title

      4(3) 4(3)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human Mena associates with Racl small GTPase in glioblastoma cell lines2009

    • Author(s)
      Higashi, M
    • Journal Title

      Plos One. 4

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 出芽酵母COPIIコートタンパク質の細胞内ダイナミクス2009

    • Author(s)
      黒川量雄
    • Organizer
      日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] 酵母Ypt GTPasesの時空間イメージング2009

    • Author(s)
      黒川量雄
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      筑波
    • Year and Date
      2009-07-29
    • Related Report
      2009 Final Research Report
  • [Presentation] 酵母Ypt GTPasesの時空間イメージング2009

    • Author(s)
      黒川量雄
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      つくば
    • Year and Date
      2009-07-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] イメージング技術を用いたYptの時空間情報解析2009

    • Author(s)
      黒川量雄
    • Organizer
      酵母細胞研究会例会
    • Place of Presentation
      船橋
    • Year and Date
      2009-07-03
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Ypt31p/32pとSec2pの相互作用の時空間イメージング2008

    • Author(s)
      黒川量雄、中野明彦
    • Organizer
      bmb2008
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report
  • [Presentation] Ypt31p/32pとSec2pの相互作用の時空間イメージング2008

    • Author(s)
      黒川量雄
    • Organizer
      BMB2008
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] 出芽酵母COPIIコートタンパク質の細胞内ダイナミクス

    • Author(s)
      黒川量雄、中野明彦
    • Organizer
      日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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