Identification and analysis of nucleocytoplasmic transport pathway using our novel assay system

• Project/Area Number: 20770166
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KOSE Shingo The Institute of Physical and Chemical Research, 今本細胞核機能研究室, 専任研究員 (90333278)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 核-細胞質間分子輸送 / 熱ショック応答 / Importin / 核内移行 / 分子シャペロン / importin / 熱ショック

Research Abstract

Previous studies suggest that the conventional nucleocytoplasmic transport, which is mediated by members of Importin β family, is down-regulated under cellular stress conditions, whereas molecular chaperones Hsc70/Hsp70 is well known to accumulate rapidly into the nucleus in response to heat shock. However, this mechanisms translocating into the nucleus remain elusive. In this study, we purified biochemically a cytosolic protein that is essential for nuclear import of Hsc70/Hsp70. Our data suggest that this molecule is a novel nuclear import receptor for Hsc70/Hsp70.

Research Products

• [Journal Article] A live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells. (2010)
  • Author(s): Iino, H., Maeshima, K., Nakatomi, R., Kose, S., Hashikawa, T., Tachibana, T., Imamoto, N.
  • Journal Title: Genes Cells (in press)
  • Peer Reviewed
• [Journal Article] Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport. (2009)
  • Author(s): Kosako, H., Yamaguchi, N., Aranami, C., Ushiyama, M., Kose, S., Imamoto, N., Taniguchi, H., Nishida, E., Hattori, S.
  • Journal Title: Nat. Struct. Mol. Biol. 16 Pages: 1026-1035
  • Peer Reviewed
• [Journal Article] Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport (2009)
  • Author(s): Kosako, H.
  • Journal Title: Nat.Struct.Mol.Biol. 16 Pages: 1026-1035
  • Peer Reviewed
• [Journal Article] Functional and structural basis of the nuclear localization signal in the ZIC3 zinc finger domain. (2008)
  • Author(s): Hatayama, M., Tomizawa, T., Sakai-Kato, K., Bouvagnet, P., Kose, S., Imamoto, N., Yokoyama, S., Utsunomiya-Tate, N., Mikoshiba, K., Kigawa, T., Aruga J.
  • Journal Title: Hum. Mol. Genet. 17 Pages: 3459-3473
  • Peer Reviewed
• [Journal Article] Functional and structural basis of the nuclear localization signal in the ZIC3 zinc finger domain (2008)
  • Author(s): Hatayama, M.
  • Journal Title: Hum. Mol. Genet. 17 Pages: 3459-3473
  • Peer Reviewed
• [Presentation] 熱ショック時における分子シャペロンHsc70の核内移行を促進するタンパク質の同定Identification of a protein facilitating nuclear import of molecular chaperone Hsc70 under heat shock (2009)
  • Author(s): 小瀬真吾、今本尚子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 2009-10-23
• [Presentation] 熱シュック時における分子シャペロンHsc70の核内移行を促進するタンパク質の同定 (2009)
  • Author(s): 小瀬真吾
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2009-10-23
• [Presentation] Analysis of nuclear import of molecular chaperone 70kDa heat shock cognate protein (Hsc70) (2009)
  • Author(s): Shingo Kose, Naoko Imamoto
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-06-02
• [Presentation] Analysis of nuclear import of molecular chaperone 70kDa heat shock cognate protein(Hsc70) (2009)
  • Author(s): 小瀬真吾
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-06-02
• [Book] 化合物で乗り物タンパク質を発見 (2008)
  • Author(s): 小瀬真吾
  • Publisher: (株)オーム社
• [Book] 細胞質から核へのタンパク質輸送 (2008)
  • Author(s): 小瀬真吾、今本尚子
  • Publisher: (株)シーエムシー出版
• [Book] 入門ケミカルバイオロジー (2008)
  • Author(s): 入門ケミカルバイオロジー編集委員会
  • Total Pages: 192
  • Publisher: 株式会社オーム社
• [Book] 機能性DDSキャリアの製剤設計 (2008)
  • Author(s): 岡田弘晃, 監修
  • Total Pages: 321
  • Publisher: シーエムシー出版
• [Remarks]
  • URL: http://www.riken.jp/r-world/research/lab/wako/cell-dyna/index.html