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Identification and analysis of nucleocytoplasmic transport pathway using our novel assay system

Research Project

Project/Area Number 20770166
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

KOSE Shingo  The Institute of Physical and Chemical Research, 今本細胞核機能研究室, 専任研究員 (90333278)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords核-細胞質間分子輸送 / 熱ショック応答 / Importin / 核内移行 / 分子シャペロン / importin / 熱ショック
Research Abstract

Previous studies suggest that the conventional nucleocytoplasmic transport, which is mediated by members of Importin β family, is down-regulated under cellular stress conditions, whereas molecular chaperones Hsc70/Hsp70 is well known to accumulate rapidly into the nucleus in response to heat shock. However, this mechanisms translocating into the nucleus remain elusive. In this study, we purified biochemically a cytosolic protein that is essential for nuclear import of Hsc70/Hsp70. Our data suggest that this molecule is a novel nuclear import receptor for Hsc70/Hsp70.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (14 results)

All 2010 2009 2008 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (4 results) Book (4 results) Remarks (1 results)

  • [Journal Article] A live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells.2010

    • Author(s)
      Iino, H., Maeshima, K., Nakatomi, R., Kose, S., Hashikawa, T., Tachibana, T., Imamoto, N.
    • Journal Title

      Genes Cells (in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport.2009

    • Author(s)
      Kosako, H., Yamaguchi, N., Aranami, C., Ushiyama, M., Kose, S., Imamoto, N., Taniguchi, H., Nishida, E., Hattori, S.
    • Journal Title

      Nat. Struct. Mol. Biol. 16

      Pages: 1026-1035

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport2009

    • Author(s)
      Kosako, H.
    • Journal Title

      Nat.Struct.Mol.Biol. 16

      Pages: 1026-1035

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional and structural basis of the nuclear localization signal in the ZIC3 zinc finger domain.2008

    • Author(s)
      Hatayama, M., Tomizawa, T., Sakai-Kato, K., Bouvagnet, P., Kose, S., Imamoto, N., Yokoyama, S., Utsunomiya-Tate, N., Mikoshiba, K., Kigawa, T., Aruga J.
    • Journal Title

      Hum. Mol. Genet. 17

      Pages: 3459-3473

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional and structural basis of the nuclear localization signal in the ZIC3 zinc finger domain2008

    • Author(s)
      Hatayama, M.
    • Journal Title

      Hum. Mol. Genet. 17

      Pages: 3459-3473

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 熱ショック時における分子シャペロンHsc70の核内移行を促進するタンパク質の同定Identification of a protein facilitating nuclear import of molecular chaperone Hsc70 under heat shock2009

    • Author(s)
      小瀬真吾、今本尚子
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸市
    • Year and Date
      2009-10-23
    • Related Report
      2009 Final Research Report
  • [Presentation] 熱シュック時における分子シャペロンHsc70の核内移行を促進するタンパク質の同定2009

    • Author(s)
      小瀬真吾
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Analysis of nuclear import of molecular chaperone 70kDa heat shock cognate protein (Hsc70)2009

    • Author(s)
      Shingo Kose, Naoko Imamoto
    • Organizer
      第61回日本細胞生物学会大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-06-02
    • Related Report
      2009 Final Research Report
  • [Presentation] Analysis of nuclear import of molecular chaperone 70kDa heat shock cognate protein(Hsc70)2009

    • Author(s)
      小瀬真吾
    • Organizer
      第61回日本細胞生物学会大会
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2009-06-02
    • Related Report
      2009 Annual Research Report
  • [Book] 化合物で乗り物タンパク質を発見2008

    • Author(s)
      小瀬真吾
    • Publisher
      (株)オーム社
    • Related Report
      2009 Final Research Report
  • [Book] 細胞質から核へのタンパク質輸送2008

    • Author(s)
      小瀬真吾、今本尚子
    • Publisher
      (株)シーエムシー出版
    • Related Report
      2009 Final Research Report
  • [Book] 入門ケミカルバイオロジー2008

    • Author(s)
      入門ケミカルバイオロジー編集委員会
    • Total Pages
      192
    • Publisher
      株式会社オーム社
    • Related Report
      2008 Annual Research Report
  • [Book] 機能性DDSキャリアの製剤設計2008

    • Author(s)
      岡田弘晃, 監修
    • Total Pages
      321
    • Publisher
      シーエムシー出版
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.riken.jp/r-world/research/lab/wako/cell-dyna/index.html

    • Related Report
      2009 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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