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Construction of a model for recovery of limb regenerative capacity in non-regenerative animals

Research Project

Project/Area Number 20770168
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionTohoku University

Principal Investigator

YOKOYAMA Hitoshi  東北大学, 大学院・生命科学研究科, 助教 (90455816)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords四肢 / 発生 / Xenopus / 再生医学 / transgenesis / 再生 / transgeneis
Research Abstract

In amphibians, Xenopus is advantageous because we can prepare numerous transgenic animals efficiently. I aimed at recovery of limb regeneration in metamorphosed Xenopus, which has only restricted regenerative capacity of a limb. I modified the gene manipulation protocols to achieve effective control of gene expression at the beginning. Then, I examined the role of Wnt/β-catenin signaling in the initiation of limb regeneration and indicated the different mechanisms of limb regeneration initiation in tadpoles and young adults with reference to Wnt/β-catenin signaling. Finally, I found that the activation of Wnt/β-catenin signaling can rescue the loss of regenerative capacity in denervated limb of a young adult frog, which cannot regenerate at all originally.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (33 results)

All 2011 2010 2009 2008 Other

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (11 results) Remarks (4 results)

  • [Journal Article] Different requirement for Wnt/β -catenin signaling in limb regeneration of larval and adult Xenopus2011

    • Author(s)
      横山仁、丸岡玉枝、越智陽城、有賀章郎、大湖史朗、荻野肇、田村宏治
    • Journal Title

      PLoS ONE

      Volume: (印刷中)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prx-1 expression in Xenopus laevis scarless skin-wound healing and its resemblance to epimorphic regeneration2011

    • Author(s)
      横山仁、丸岡玉枝、有賀章郎、天野孝紀、大湖史朗、城石俊彦、田村宏治
    • Journal Title

      Journal of Investigative Dermatology

      Volume: (印刷中)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of paraxial mesoderm in limb/flank regionalization of the trunk lateral plate2011

    • Author(s)
      野呂美幸、湯口弘樹、佐藤妙子、對比地孝亘、米井(田村)小百合、横山仁、若松義雄、田村宏治
    • Journal Title

      Developmental Dynamics

      Volume: (印刷中)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Embryological evidence identifies wing digits in birds as digits 1, 2, and 32011

    • Author(s)
      田村宏治、野村直生、関亮平、米井(田村)小百合、横山仁
    • Journal Title

      Science

      Volume: Vol.331 Pages: 753-757

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Embryological evidence identifies wing digits in birds as digits 1, 2, and 32011

    • Author(s)
      田村宏治、野村直生、関亮平、米井(田村)小百合、横山仁
    • Journal Title

      Science

      Volume: 331 Pages: 753-757

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of paraxial mesoderm in limb/flank regionalization of the trunk lateral plate2011

    • Author(s)
      野呂美幸、湯口弘樹、佐藤妙子、對比地孝亘, 米井(田村)小百合、横山仁、若松義雄、田村宏治
    • Journal Title

      Developmental Dynamics

      Volume: (印刷中)(未定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphogenetic change of the limb bud in the hand plate formation2010

    • Author(s)
      佐藤耕世、関亮平、野呂美幸、横山仁、田村宏治
    • Journal Title

      Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

      Volume: Vol.314B Pages: 539-551

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Limb blastema cell: a stem cell for morphological regeneration2010

    • Author(s)
      田村宏治、大湖史朗、横山仁
    • Journal Title

      Development Growth and Differentiation

      Volume: Vol.52 Pages: 89-99

    • NAID

      10027563223

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of hoxa11 and hoxa13 expression during patternless limb regeneration in Xenopus2010

    • Author(s)
      大湖史朗、伊藤あかり、鈴木誠、佐藤伸、横山仁、田村宏治
    • Journal Title

      Developmental Biology

      Volume: Vol.338 Pages: 148-157

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anterior shift in gene expression precedes anteriormost digit formation in amniote limbs2010

    • Author(s)
      上嶋朝佳、天野孝紀、野村直生、野呂美幸、安江泰治、城石俊彦、太田訓正、横山仁、田村宏治
    • Journal Title

      Development, Growth and Differentiation

      Volume: Vol.52 Pages: 223-234

    • NAID

      10027563906

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Morphogenetic change of the limb bud in the hand plate formation2010

    • Author(s)
      佐藤耕世、関亮平、野呂美幸、横山仁、田村宏治
    • Journal Title

      Journal of Experimental Zoology Part B : Molecular and Developmental Evolution

      Volume: 314B Pages: 539-551

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anterior shift in gene expression precedes anteriormost digit formation in amniote limbs2010

    • Author(s)
      上嶋朝佳、天野孝紀、野村直生、野呂美幸、安江泰治、城石俊彦、太田訓正、横山仁、田村宏治
    • Journal Title

      Development, Growth and Differentiation 52

      Pages: 223-234

    • NAID

      10027563906

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of hoxa11 and hoxa13 expression during patternless limb regeneration in Xenopus2010

    • Author(s)
      大湖史朗、伊藤あかり、鈴木誠、佐藤伸、横山仁、田村宏治
    • Journal Title

      Developmental Biology 338

      Pages: 148-157

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Limb blastema cell : a stem cell for morphological regeneration2010

    • Author(s)
      田村宏治、大湖史朗、横山仁
    • Journal Title

      Development Growth and Differentiation 52

      Pages: 89-99

    • NAID

      10027563223

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Repatterning in amphibian limb regeneration: a model for study of genetic and epigenetic control of organ regeneration2009

    • Author(s)
      薬師寺那由他、横山仁、田村宏治
    • Journal Title

      Seminars in Cell and Developmental Biology

      Volume: Vol.20 Pages: 565-578

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The autopod: its formation during limb development2008

    • Author(s)
      田村宏治、米井(田村)小百合、矢野十織、横山仁、井出宏之
    • Journal Title

      Growth and Differentiation

      Volume: Vol.50

    • NAID

      10024399869

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The autopod : its formation during limb development2008

    • Author(s)
      田村広治、米井-田村小百口、矢野十織、横山仁、井出宏之
    • Journal Title

      Development, Growth and Differentiation 50

    • NAID

      10024399869

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Repatterning in amphibian limb regeneration : a model for study of gene tic and epigenetic control of organ regeneration2008

    • Author(s)
      薬師寺那由他、横山仁、田村宏治
    • Journal Title

      Seminars in Cell and Developmental Biology 20(印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Limb regeneration of Xenopus as a model for three-dimensional appendage/ organ regeneration2011

    • Author(s)
      横山仁
    • Organizer
      The 2nd UCL-Tohoku University Symposium “From Cell/Developmental Biology to Neuroscience”
    • Place of Presentation
      仙台
    • Year and Date
      2011-01-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Limb regeneration of Xenopus as a model for three-dimensional appendage/organ regeneration2011

    • Author(s)
      横山仁
    • Organizer
      The 2nd UCL-Tohoku University Symposium "From Cell/Developmental Biology to Neuroscience"
    • Place of Presentation
      仙台
    • Year and Date
      2011-01-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Different involvement of Wnt/β-catenin signaling in limb regeneration of larval and adult Xenopus2010

    • Author(s)
      横山仁、丸岡玉枝、越智陽城、有賀章郎、大湖史朗、荻野肇、田村宏治
    • Organizer
      EMBO Conference on the Molecular & Cellular Basis of Regeneration & Tissue Repair
    • Place of Presentation
      セジンブラ、ポルトガル
    • Year and Date
      2010-09-29
    • Related Report
      2010 Final Research Report
  • [Presentation] Different involvement of Wnt/β-catenin signaling in limb regeneration larval and adult Xenopus2010

    • Author(s)
      横山仁、丸岡玉枝、越智陽城、有賀章郎、大湖史朗、荻野肇、田村宏治
    • Organizer
      EMBO Conference on the Molecular & Cellular Basis of Regeneration & Tissue Repair
    • Place of Presentation
      セジンブラ、ポルトガル
    • Year and Date
      2010-09-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 幼生期と成体期の Xenopus でみられる四肢再生における異なる開始機構2010

    • Author(s)
      横山仁、丸岡玉枝、越智陽城、有賀章郎、大湖史朗、荻野肇、田村宏治
    • Organizer
      日本動物学会東北支部大会
    • Place of Presentation
      福島
    • Year and Date
      2010-08-07
    • Related Report
      2010 Final Research Report
  • [Presentation] 幼生期と成体期のXenopusでみられる四肢再生における異なる開始機構2010

    • Author(s)
      横山仁、丸岡玉枝、越智陽城、有賀章郎、大湖史朗、荻野肇、田村宏治
    • Organizer
      日本助物学会東北支部大会
    • Place of Presentation
      福島
    • Year and Date
      2010-08-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 幼生期と成体期のアフリカツメガエルでみられる四肢再生における異なる開始機構(Different mechanisms in initiation of limb regeneration between larval and adult Xenopus)2010

    • Author(s)
      横山仁、大湖史朗、有賀章郎、丸岡玉枝、越智陽城、荻野肇、田村宏治
    • Organizer
      日本発生生物学会
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report
  • [Presentation] 幼生期と成体期のアフリカツメガエルでみられる四肢再生における異なる開始機構(Different me anisms in initiation of limb regeneration between larval and adult Xenopus)2010

    • Author(s)
      横山仁、大湖史朗、有賀章郎、丸岡玉枝、越智陽城、荻野肇、田村宏治
    • Organizer
      日本発生生物学会
    • Place of Presentation
      京都
    • Related Report
      2010 Annual Research Report
  • [Presentation] nt シグナリングは四肢再生に対して幼生期と成体期で異なる関与をする2009

    • Author(s)
      横山仁、越智陽城、荻野肇、田村宏治
    • Organizer
      日本動物学会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-19
    • Related Report
      2010 Final Research Report
  • [Presentation] Wntシグナリングは四肢再生に対して幼生期と成体期で異なる関与をする2009

    • Author(s)
      横山仁、越智陽城、荻野肇、田村宏治
    • Organizer
      日本動物学会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Wnt/β-catenin シグナリングは四肢再生に対して幼生期と成体期で異なる関与をする2009

    • Author(s)
      横山仁、越智陽城、荻野肇、田村宏治
    • Organizer
      日本発生生物学会
    • Place of Presentation
      新潟
    • Year and Date
      2009-05-29
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/tamlab/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/tamlab/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/tamlab/index.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/tamlab/index.html

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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