Temporal and spatial regulation of competence in ascidian embryonic induction

• Project/Area Number: 20770178
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Osaka University
• Principal Investigator: KUMANO Gaku Osaka University, 大学院・理学研究科, 助教 (80372605)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 初期発生 / ホヤ / 誘導 / 応答能

Research Abstract

Molecular mechanisms by which signal-receiving cells respond to the same inductive signal differently to make different tissue types in development were analyzed using ascidian embryos. We found that posteriorly-localized maternal PEM makes different competence factor expression between the anterior and posterior regions of the embryo and that these region (or cell)-specific competence factors in cooperation with the inductive signal generate different tissue types. In addition, we identified FoxB as a factor responsible for the loss of competence to the above inductive signal.

Research Products

• [Journal Article] Macho-1 regulates unequal cell divisions independently of its function as a muscle determinant (2010)
  • Author(s): Kumano, G., Kawai, N., Nishida, H.
  • Journal Title: Dev. Biol. (in press)
  • Peer Reviewed
• [Journal Article] Spatial and temporal expression of two transcriptional isoforms of Lhx3, a LIM class homeobox gene, during embryogenesis of two phylogenetically remote ascidians, Halocynthia roretzi and Ciona intestinalis (2010)
  • Author(s): Kobayashi, M., Takatori, N., Nakajima, Y., Kumano, G., Nishida, H., Saiga, H.
  • Journal Title: Gene Expr. Patterns 10 Pages: 98-104
  • Peer Reviewed
• [Journal Article] Spatial and temporal expression of two transcriptional isoforms of Lhx 3, aLIM class homeobox gene, during embryogenesis of two phylogenetic ally remote ascidian, Ha/ocynthia roretZi and Cioaa intestinalis (2010)
  • Author(s): Kobayashi, M.
  • Journal Title: Gene Expr.Patterns (in press)
  • Peer Reviewed
• [Journal Article] Wnt5 is required for notochord cell intercalation in the ascidian Halocynthia roretzi (2009)
  • Author(s): Niwano, T., Taktori, N., Kumano, G., Nishida, H.
  • Journal Title: Biol. Cell 101 Pages: 645-659
  • Peer Reviewed
• [Journal Article] Patterning of an ascidian embryo along the anterior-posterior axis through spatial regulation of competence and induction ability by maternally localized PEM (2009)
  • Author(s): Kumano, G., Nishida, H.
  • Journal Title: Dev. Biol. 331 Pages: 78-88
  • Peer Reviewed
• [Journal Article] Patterning of an ascidian embryo along the anterior-posterior axis through spatial regulation of competence and induction ability by maternally localizaed PEM (2009)
  • Author(s): Kumanno, G.
  • Journal Title: Dev.Biol. 331 Pages: 78-88
  • Peer Reviewed
• [Journal Article] Wnt5 is required for notochord cell intercalation in the ascidian Ha/ocynthia roretzi (2009)
  • Author(s): Niwano, T.
  • Journal Title: Biol.Cell 101 Pages: 645-659
  • Peer Reviewed
• [Presentation] Spatial and temporal regulation of competence to respond to the FGF signal in ascidian embryonic induction (2009)
  • Author(s): Kumano, G.
  • Organizer: Special seminar in National Tsing Hua University-Osaka University Life Science Student Activity Fair 2009
  • Place of Presentation: Taiwan
  • Year and Date: 2009-05-12
• [Presentation] Spatial and temporal regulation of competence to respond to the FGF signal in ascidian embryonic induction (2009)
  • Author(s): Kumano, G
  • Organizer: National Tsing Hua University-Osaka University Life Science Student Activity Fair
  • Place of Presentation: 新竹市(台湾)
  • Year and Date: 2009-05-12
• [Presentation] ホヤ胚誘導における時間的・空間的応答能制御機構の解析 (2009)
  • Author(s): 熊野岳
  • Organizer: 日本発生生物学会秋季シンポジウム
  • Place of Presentation: 静岡
• [Presentation] マボヤ初期発生におけるTwist-like遺伝子の発現・機能解析 (2009)
  • Author(s): 児玉仁、根来延枝、熊野岳、西田宏記
  • Organizer: 第80回日本動物学会
  • Place of Presentation: 静岡
• [Presentation] マボヤ胚発生における発生運命と細胞分裂回数の関係 (2009)
  • Author(s): 藤川徹也、熊野岳、西田宏記
  • Organizer: 第80回日本動物学会
  • Place of Presentation: 静岡
• [Presentation] Left-right asymmetry of ascidian larvae is determined by rotation of the whole embryos within the vitelline membrane (2009)
  • Author(s): Mugitani, M., Nishide, K., Kumano, G., Nishida, H
  • Organizer: 16th International Society of Developmental Biologists Congress, Edinburgh
  • Place of Presentation: Scotland, United Kingdom
• [Presentation] Molecular basisi of attenuation of competence to respond to FGF signal in ascidian notochord induction (2009)
  • Author(s): Hashimoto, H., Enomoto, A., Kumano, G., Nishida, H.
  • Organizer: 16th International Society of Developmental Biologists Congress
  • Place of Presentation: Edinburgh, Scotland, United Kingdom
• [Presentation] Patterning of an ascidian embryo along the anterior-posterior axis through spatial regulation of competence and induction ability by PEM (2009)
  • Author(s): Kumano, G., Nishida, H.
  • Organizer: 5th International Tunicate Meeting
  • Place of Presentation: Okinawa, Japan
• [Presentation] Screening for the proteins interacting with PEM sheds light on the Plk1 (2009)
  • Author(s): Negishi, T., Kumano, G., Nishida, H.
  • Organizer: 5th International Tunicate Meeting
  • Place of Presentation: Okinawa, Japan
• [Presentation] Molecular basis of attenuation of competence to respond to FGF signal in ascidian notochord induction (2009)
  • Author(s): Hashimoto, H., Enomoto, A., Kumano, G., Nishida, H.
  • Organizer: 5th International Tunicate Meeting
  • Place of Presentation: Okinawa, Japan
• [Presentation] Wnt-dependent asymmetric partitioning of Not mRNA segregates mesoderm and endoderm fates in ascidian embryos (2009)
  • Author(s): Takatori, N., Kumano, G., Saiga, H., Nishida, H.
  • Organizer: 5th International Tunicate Meeting
  • Place of Presentation: Okinawa, Japan
• [Presentation] Molecular basis of loss of competence to the FGF signal in ascidian notochord induction (2009)
  • Author(s): Hashimoto, H., Enomoto, A., Kumano, G., Nishida, H.
  • Organizer: 第42回日本発生生物学会
  • Place of Presentation: 新潟
• [Presentation] Analysis of the expression of Lhx3 genes in two ascidian species, Halocynthia roretzi and CIona intestinalis (2009)
  • Author(s): Kobayashi, M., Takatori, N., Nakjima, Y., Kumano, G., Nishida, H., Saiga, H.
  • Organizer: 第42回日本発生生物学会
  • Place of Presentation: 新潟
• [Presentation] Polarization of embryonic cells by prelocalized factors and external signals in ascidians (2009)
  • Author(s): Nishida, H., Prodon, F., Takatori, N., Kim, G.J., Kumano, G.
  • Organizer: CDB symposium 2009, Shape and Polarity
  • Place of Presentation: Kobe, Japan
• [Presentation] Wnt segregates mesoderm and endoderm fates via asymmetric inheritance of Not mRNA in ascidian embryo (2009)
  • Author(s): Takatori, N., Kumano, G., Saiga, H., Nishida, H.
  • Organizer: CDB symposium 2009, Shape and Polarity
  • Place of Presentation: Kobe, Japan
• [Presentation] Analysis of the expression of Lhx3 genes in two ascidian species, Halocynthia roretzi and Clona in testinalis (2009)
  • Author(s): Kobayashi, M.
  • Organizer: 第42回日本発生生物学会
  • Place of Presentation: 朱鷺メッセ(新潟)
• [Presentation] Molecular basis of loss of competence to the FGF signal in ascidian notochord induction (2009)
  • Author(s): Hashimoto, H.
  • Organizer: 第42回日本発生生物学会
  • Place of Presentation: 朱鷺メッセ(新潟)
• [Presentation] Left-right asymmetry of ascidian larvae is determined by rotati on of the whole embryos within the vitelline membrane (2009)
  • Author(s): Mugi tani, M.
  • Organizer: 16th International Society of Developmental Biologists Congress
  • Place of Presentation: Edinburgh, Scotland, United Kingdom
• [Presentation] Wnt-dependent asymmetric partitioning of Not mRNA segregates mesoderm and endoderm fates in ascidian embryos (2009)
  • Author(s): Takatori, N.
  • Organizer: 5th International Tunicate Meeting
  • Place of Presentation: 沖縄産業支援センター(沖縄)
• [Presentation] Molecular basis of attenuation of competence to respond to FGF signal in ascidian notochord induction (2009)
  • Author(s): Hashimoto, H
  • Organizer: 5th International Tunicate Meeting
  • Place of Presentation: 沖縄産業支援センター(沖縄)
• [Presentation] Screening for the proteins interacting with PEM sheds light on the Plk1 (2009)
  • Author(s): Negishi, T.
  • Organizer: 15th International Tunicate Meeting
  • Place of Presentation: 沖縄産業支援センター(沖縄)
• [Presentation] Patterning of an ascidian embryo along the anterior-posterior a xis through spatial regulation of competence and induction ability by PEM (2009)
  • Author(s): Kumano, G.
  • Organizer: 5th International Tunicate Meeting
  • Place of Presentation: 沖縄産業支援センター(沖縄)
• [Presentation] マボヤ胚発生における発生運命と細胞分裂回数の関係 (2009)
  • Author(s): 藤川徹也
  • Organizer: 第80回日本動物学会
  • Place of Presentation: 静岡グランシップ(静岡)
• [Presentation] マボヤ初期発生におけるTwist-like遺伝子の発現・機能解析 (2009)
  • Author(s): 児玉仁
  • Organizer: 第80回日本動物学会
  • Place of Presentation: 静岡グランシップ(静岡)
• [Presentation] ホヤ胚誘導における時間的・空間的応答能制御機構の解析 (2009)
  • Author(s): 熊野岳
  • Organizer: 日本発生生物学会秋季シンポジウム
  • Place of Presentation: 東レ研修センター(静岡)
• [Presentation] 発生運命決定にかかわるRNAのWnt依存的な非対称分配機構の解析 (2008)
  • Author(s): 高鳥直士、熊野岳、西田宏記
  • Organizer: 第79回日本動物学会
  • Place of Presentation: 福岡