Planar cell polarity of multi-ciliated ependymal cells

• Project/Area Number: 20770182
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Nagoya City University
• Principal Investigator: HIROTA Yuki Nagoya City University, 大学院・医学研究科, 助教 (00453548)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞分化 / 繊毛 / 神経科学 / 上衣細胞 / 発生・分化 / 再生医学

Research Abstract

Motile cilia generate constant fluid flow over epithelial tissue, and thereby influence diverse physiological processes. Such functions of ciliated cells depend on the planar polarity of the ciliated cells. Here we report that basal bodies move in the apical cell membrane during differentiation to accumulate in the anterior region of ependymal cells that line the surface of brain ventricles. Moreover, we show that non-muscle myosin II is a key regulator of this distribution of basal bodies.

Research Products

• [Journal Article] Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain. (2011)
  • Author(s): Sawamoto, K., Hirota, Y., Alfaro-Cervello, C., Soriano-Navarro, M., He, X., Hayakawa-Yano, Y., Yamada, M., Hikishima, K., Tabata, H., Iwanami, A., Nakajima, K., Toyama, Y., Itoh, T., Alvarez-Buylla, A., Garcia-Verdugo, J.M., Okano, H.
  • Journal Title: J Comp Neurol (K.Sawamoto, Y.Hirota, C.Alfaro-Cervello, M.Soriano-Navarro contributed equallyto this work) 519(4) Pages: 690-713
  • Peer Reviewed
• [Journal Article] Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain. (2011)
  • Author(s): Sawamoto, K., Hirota, Y., Alfaro-Cervello, C., Soriano-Navarro, M., He, X., Hayakawa-Yano, Y., Yamada, M., Hikishima, K., Tabata, H., Iwanami, A., Nakajima, K., Toyama, Y., Itoh, T., Alvarez-Buylla, A., Garcia-Verdugo, J.M., Okano, H.
  • Journal Title: J Comp Neurol Volume: 519 Pages: 690-713
  • Peer Reviewed
• [Journal Article] Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II. (2010)
  • Author(s): Hirota, Y., Meunier, A., Huang, S., Shimozawa, T., Yamada, O., Kida, Y.S., Inoue, M., Ito, T., Kato, H., Sakaguchi, M., Sunabori, T., Nakaya, M.A., Nonaka, S., Ogura, T., Higuchi, H., Okano, H., Spassky, N., Sawamoto, K.
  • Journal Title: Development 137(18) Pages: 3037-3046
  • Peer Reviewed
• [Journal Article] Coupling between hydrodynamic forces and planar cell polarity orients mammalian motile cilia. (2010)
  • Author(s): Guirao, B., Meunier, A., Mortaud, S., Aguilar, A., Corsi, J.M., Strehl, L., Hirota, Y., Desoeuvre, A., Boutin, C., Han, Y.G., Mirzadeh, Z., Cremer, H., Montcouquiol, M., Sawamoto, K., Spassky, N.
  • Journal Title: Nat.Cell Biol. 12 Pages: 341-350
  • Peer Reviewed
• [Journal Article] Expression and proliferation-promoting role of Diversin in the neuronally committed precursor cells migrating in the adult mouse brain. (2010)
  • Author(s): Ikeda, M., Hirota, Y., Sakaguchi, M., Yamada, O., Kida, Y.S., Ogura, T., Otsuka, T., Okano, H., Sawamoto, K.
  • Journal Title: Stem Cells 28(11) Pages: 2017-2026
  • Peer Reviewed
• [Journal Article] Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II. (2010)
  • Author(s): Hirota, Y., Meunier, A., Huang, S., Shimozawa, T., Yamada, O., Kida, Y.S., Inoue, M., Ito, T., Kato, H., Sakaguchi, M., Sunabori, T., Nakaya, M.A., Nonaka, S., Ogura, T., Higuchi, H., Okano, H., Spassky, N., Sawamoto, K.
  • Journal Title: Development Volume: 137 Pages: 3037-3046
  • Peer Reviewed
• [Journal Article] Expression and proliferation-promoting role of Diversin in the neuronally committed precursor cells migrating in the adult mouse brain. (2010)
  • Author(s): Ikeda, M., Hirota, Y., Sakaguchi, M., Yamada, O., Kida, Y.S., Ogura, T., Otsuka, T., Okano, H., Sawamoto, K.
  • Journal Title: Stem Cells Volume: 28 Pages: 2017-2026
  • Peer Reviewed
• [Journal Article] Coupling between hydrodynamic forces and planar cell polarity orients mammalian motile cilia. (2010)
  • Author(s): Guirao, B., Meunier, A., Mortaud, S., Aguilar, A., Corsi, J.M., Strehl, L., Hirota, Y., Desoeuvre, A., Boutin, C., Han, Y.G., Mirzadeh, Z., Cremer, H., Montcouquiol, M., Sawamoto, K., Spassky, N
  • Journal Title: Nat Cell Biol 12 Pages: 341-350
  • Peer Reviewed
• [Journal Article] Subventricular Zone-Derived Neural Progenitor Cells Migrate Along a Blood Vessel Scaffold Toward the Post-Stroke Striatum. (2010)
  • Author(s): Kojima, T., Hirota, Y., Ema, M., Takahashi, S., Miyoshi, I., Okano, H., Sawamoto, K
  • Journal Title: Stem Cells 28 Pages: 545-554
  • Peer Reviewed
• [Journal Article] 中枢神経系における繊毛の機能 (2010)
  • Author(s): 廣田ゆき, 澤本和延
  • Journal Title: 小児の脳神経 35 Pages: 11-14
  • NAID: 10026528588
• [Journal Article] Efhc1 deficiency causes spontaneous myoclonus and increased seizure susceptibility. (2009)
  • Author(s): Suzuki, T., Miyamoto, H., Nakahari, T., Inoue, I., Suemoto, T., Jiang, B., Hirota, Y., Itohara, S., Saido, TC., Tsumoto, T., Sawamoto, K., Hensch, TK., Delgado-Escueta, AV., Yamakawa, K.
  • Journal Title: Hum.Mol.Genet. 18 Pages: 1099-1109
  • Peer Reviewed
• [Journal Article] Various facets of vertebrate cilia : motility, signaling, and role in adult neurogenesis. (2009)
  • Author(s): Huang, S., Hirota, Y., Sawamoto, K.
  • Journal Title: Proceedings of the Japan Academy, Ser. B85 Pages: 324-336
  • NAID: 130000138037
  • Peer Reviewed
• [Journal Article] Various facets of vertebrate cilia : motility, signaling, and role in adult neurogenesis (2009)
  • Author(s): Huang, S., Hirota, Y., Sawamoto, K
  • Journal Title: Proceedings of the Janpan Academy, Ser.B 85 Pages: 324-336
  • NAID: 130000138037
  • Peer Reviewed
• [Journal Article] 成体脳におけるニューロン新生 (2009)
  • Author(s): 黄詩恵, 廣田ゆき, 澤本和延
  • Journal Title: 細胞工学 28 Pages: 1016-1020
• [Journal Article] 中枢神経系における繊毛の機能 (2009)
  • Author(s): 小島拓郎, 廣田ゆき, 澤本和延
  • Journal Title: 慶應医学 85 Pages: 169-177
  • NAID: 10026528588
• [Journal Article] Ethel deficiency causes spontaneous myoclonus and increased seizure susceptibility (2009)
  • Author(s): Suzuki T, Miyamoto H, Nakahari T, Inoue I, Suemoto T, Jiang B, Hirota Y, Itohara S, Saido TC, Tsumoto T, Sawamoto K, Hensch TK, Delgado-Escueta AV, Yamakawa K.
  • Journal Title: Hum Mol Genet 18 Pages: 1099-1109
  • Peer Reviewed
• [Journal Article] ニューロンの移動制御と細胞接着 (2009)
  • Author(s): 廣田ゆき, 澤本和延
  • Journal Title: 実験医学 26 Pages: 2191-2195
• [Journal Article] 成体脳室下帯で誕生し移動するニューロン (2008)
  • Author(s): 廣田ゆき, 澤本和延
  • Journal Title: 蛋白質核酸酵素 53 Pages: 863-869
• [Presentation] Planar polarity of multiciliated ependymal cells on the lateral ventricular wall of mouse brain regulated by non-muscle myosin II (2010)
  • Author(s): Hirota, Y., Meunier, A., Huang, S., Shimozawa, T., Yamada, O., Kida, Y.S., Inoue, M., Ito, T., Kato, H., Sakaguchi, M., Sunabori, T., Nakaya, M.A., Nonaka, S., Ogura, T., Higuchi, H., Okano, H., Spassky, N., Sawamoto, K.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Planar polarity of multiciliated ependymal cells on the lateral ventricular wall of mouse brain regulated by non-muscle myosin II (2010)
  • Author(s): Hirota, Y., Meunier, A., Huang, S., Shimozawa, T., Yamada, O., Kida, Y.S., Inoue, M., Ito, T., Kato, H., Sakaguchi, M., Sunabori, T., Nakaya, M.A., Nonaka, S., Ogura, T., Higuchi, H., Okano, H., Spassky, N., Sawamoto, K.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] Planar cell polarity of multiciliated ependymal cells regulated by non-muscle myosin II. (2010)
  • Author(s): Hirota, Y., Meunier, A., Huang, S., Shimozawa, T., Yamada, O., Kida, Y.S., Inoue, M., Ito, T., Kato, H., Sakaguchi, M., Sunabori, T., Nakaya, M.A., Nonaka, S., Ogura, T., Higuchi, H., Okano, H., Spassky, N., Sawamoto, K.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] Planar cell polarity of multiciliated ependymal cells regulated by non-muscle myosin II. (2010)
  • Author(s): Hirota, Y., Meunier, A., Huang, S., Shimozawa, T., Yamada, O., Kida, Y.S., Inoue, M., Ito, T., Kato, H., Sakaguchi, M., Sunabori, T., Nakaya, M.A., Nonaka, S., Ogura, T., Higuchi, H., Okano, H., Spassky, N., Sawamoto, K.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-09-02
• [Presentation] Planar polarization of ependymal cells on the ventricular wall of mouse lateral ventricles. (2009)
  • Author(s): Hirota, Y., Huang, S., Yamada, O., Kida, YS., Sakaguchi, M., Nakaya, M., Spassky, N., Shimozawa, T., Higuchi, H., Ogura, T., Nonaka, S., Okano, H., Sawamoto.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Planar polariation of ependymal cells on the ventricular wall of mouse lateral ventricles (2009)
  • Author(s): Yuki Hirota, Shihhui Huang, Osamu Yamada, Yasuyuki Kida, Masanori Sakaguchi, Takehiko Sunabori, Masa-aki Nakaya, Nathalie Spassky, Togo Shimozawa, Hideo Higuchi, Toshihiko Ogura, Shigenori Nonaka, Hideyuki Okano, Kazunobu Sawamoto
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (神奈川県)
  • Year and Date: 2009-12-11
• [Presentation] マウス発達期における脳室上衣細胞繊毛の平面極性獲得機構 (2009)
  • Author(s): 廣田ゆき, 黄詩惠, 澤本和延
  • Organizer: 第2回日本水頭症脳脊髄液学会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-14
• [Presentation] Motility analysis with nm-accuracy and high temporal resolution of mice ependymal cilia by confocal imaging. (2009)
  • Author(s): Shimozawa, T., Hirota, Y., Sawamoto, K., Higuchi, H.
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-11-01
• [Presentation] Polarization of ependymal cilia required for fluid flow in the adult mouse lateral ventricles. (2009)
  • Author(s): Hirota, Y., Huang, S., Yamada, O., Kida, Y.S., Sakaguchi, M., Nakaya, M., Spassky, N., Shimozawa, T., Higuchi, H., Ogura, T., Nonaka, S., Okano, H., Sawamoto.
  • Organizer: Construction and Reconstruction of the Brain
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-09
• [Presentation] Plarization of ependymal cilia required for fluid flow in the a dult mouse lateral ventricles (2009)
  • Author(s): Yuki Hirota, Shihhui Huang, Osamu Yamada, Yasuyuki Kida, Masanori Sakaguchi, Takehiko Sunabori, Masa-aki Nakaya, Nathalie Spassky, Togo Shimozawa, Hideo Higuchi, Toshihiko Ogura, Shigenori Nonaka, Hideyuki Okano, Kazunobu Sawamoto
  • Organizer: 国際シンポジウム"Construction and Reconstruction of the Brain"
  • Place of Presentation: 淡路島夢舞台 (兵庫県)
  • Year and Date: 2009-10-09
• [Presentation] Planar polarity decisions for directional beating of ependymal cilia and fluid flow in the adult mouse lateral ventricles. (2009)
  • Author(s): Hirota, Y., Huang, S., Yamada, O., Kida, YS., Sakaguchi, M., Nakaya, M., Spassky, N., Shimozawa, T., Higuchi, H., Ogura, T., Nonaka, S., Okano, H., Sawamoto, K.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] Planar polarity decisions for directional beating of ependymal cilia and fluid flow in the adult mouse lateral ventricles (2009)
  • Author(s): Yuki Hirota, Shihhui Huang, Osamu Yamada, Yasuyuki Kida, Kasanori Sakaguchi, Mesa-aki Nakaya, Nathalie Spassky, Togo Shimozawa, Hideo Higuchi, Toshihiko Ogura, Shigenori Nonaka, Hideyuki Okano, Kazunobu Sawamoto
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場 (愛知県)
  • Year and Date: 2009-09-17
• [Presentation] Function of Cdk5 in neuroblast migration in the postnatal subventricular zone (2008)
  • Author(s): Yuki Hirota, Toshio Ohshima, Naoko Kaneko, Makiko Ikeda, Takuji Iwasato, Ashok B. Kulkarni, Katsuhiko Mikoshiba, Hideyuki Okano. Kazunobu Sawamoto
  • Organizer: 北米神経科学学会 (Society for Neuroscience)
  • Place of Presentation: Washington DC
  • Year and Date: 2008-11-16
• [Presentation] Polarization of ependymal cilia during mouse brain development. (2008)
  • Author(s): Hirota, Y., Huang, S., Yamada, O., Kida, Y.S., Sakaguchi, M., Nakaya, M.,Spassky, N., Shimozawa, T., Higuchi, H., Ogura, T., Nonaka, S., Okano, H., Sawamoto, K.
  • Organizer: 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: 東京
  • Year and Date: 2008-11-01
• [Book] Neurogenesis in the adult brain (2011)
  • Author(s): Sawada, M., Huang, S., Hirota, Y., Kaneko, N., Sawamoto, K.
  • Publisher: Springer(in press)
• [Book] Neurogenesis in the adult brain. (Neuronal Migration in The Adult Brain.) In press.
  • Author(s): Sawada, M., Huang, S., Hirota, Y., Kaneko, N., Sawamoto, K.
  • Publisher: Springer
• [Remarks]
  • URL: http://k-sawamoto.com/
• [Remarks] 名古屋市立大学大学院医学研究科再生医学分野のホームページ
  • URL: http://k-sawamoto.com/
• [Remarks] 名古屋市立大学大学院医学研究科再生医学分野のホームページ
  • URL: http://k-sawamoto.com/