Development of a temporally regulable gene expression system in ascidian embryos

Research Project

Project/Area Number	20770183
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Developmental biology
Research Institution	Nagahama Institute of Bio-Science and Technology
Principal Investigator	WADA Shuichi Nagahama Institute of Bio-Science and Technology, バイオサイエンス学部, 講師 (20378607)
Project Period (FY)	2008 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	遺伝子発現調節 / 熱ショック応答 / 転写制御領域 / 誘導発現系
Research Abstract	So far there was no inducible gene expression system in the ascidian Ciona intestinalis. We developed a heat shock-inducible gene expression system based on the cis-regulatory region of the Ciona HSP70 family gene Ci-HSPA1/6/7-like. This system allowed temporally controlled induction of gene expression in ascidian embryos. We also showed that heat shock response of Ci-HSPA1/6/7-like is mediated by the heat shock transcription factor.

Report

(3 results)