Development of a temporally regulable gene expression system in ascidian embryos
Project/Area Number |
20770183
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Developmental biology
|
Research Institution | Nagahama Institute of Bio-Science and Technology |
Principal Investigator |
WADA Shuichi Nagahama Institute of Bio-Science and Technology, バイオサイエンス学部, 講師 (20378607)
|
Project Period (FY) |
2008 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 遺伝子発現調節 / 熱ショック応答 / 転写制御領域 / 誘導発現系 |
Research Abstract |
So far there was no inducible gene expression system in the ascidian Ciona intestinalis. We developed a heat shock-inducible gene expression system based on the cis-regulatory region of the Ciona HSP70 family gene Ci-HSPA1/6/7-like. This system allowed temporally controlled induction of gene expression in ascidian embryos. We also showed that heat shock response of Ci-HSPA1/6/7-like is mediated by the heat shock transcription factor.
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Report
(3 results)
Research Products
(2 results)